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Abstract:

In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using Rhinella arenarum, we have performed, for the first time, the matrix assisted-ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted-ultraviolet laser desorption plate for analysis. Electrospray ionization-Orbitrap was used for cross-checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non-reproductive, reproductive), and season (non-reproductive, reproductive) are described. Copyright © 2018 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation
Autor:Petroselli, G.; Raices, M.; Jungblut, L.D.; Pozzi, A.G.; Erra-Balsells, R.
Filiación:Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Pabellón II, 3er P., Ciudad Universitaria, Buenos Aires, 1428, Argentina
CONICET, Universidad de Buenos Aires. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales, Pabellón II, 3er P. Ciudad Universitaria, Buenos Aires, 1428, Argentina
CONICET, Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), Facultad de Ciencias Exactas y Naturales, Pabellón II, 4to P., Ciudad Universitaria, Buenos Aires, 1428, Argentina
Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Pabellón II, 4to P., Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:bufadienolides; fingerprint; MALDI-MS; parotoid secretion; Rhinella arenarum; Amines; Desorption; Electrospray ionization; Esters; Mass spectrometry; Physiology; Proteins; bufadienolides; fingerprint; MALDI-MS; parotoid secretion; Rhinella arenarum; Ultraviolet lasers; argininylbufadienolide ester; bufadienolide derivative; ester; unclassified drug; arginine; bufadienolide derivative; ester; adult; age; Anura; Article; chemical composition; controlled study; desorption; electrospray mass spectrometry; female; male; matrix-assisted laser desorption-ionization mass spectrometry; nonhuman; parotid gland; priority journal; Rhinella arenarum; saliva analysis; salivation; seasonal variation; sex difference; tandem mass spectrometry; ultraviolet radiation; analogs and derivatives; animal; Chordata; growth, development and aging; high performance liquid chromatography; matrix-assisted laser desorption-ionization mass spectrometry; metabolism; parotid gland; physiology; principal component analysis; procedures; season; secretion (process); Animals; Arginine; Bufanolides; Chordata; Chromatography, High Pressure Liquid; Esters; Female; Male; Parotid Gland; Principal Component Analysis; Seasons; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry
Año:2018
Volumen:53
Número:6
Página de inicio:465
Página de fin:475
DOI: http://dx.doi.org/10.1002/jms.4082
Título revista:Journal of Mass Spectrometry
Título revista abreviado:J. Mass Spectrom.
ISSN:10765174
CODEN:JMSPF
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; Arginine; Bufanolides; Esters
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v53_n6_p465_Petroselli

Referencias:

  • Frost, D.R., (2016) Amphibian Species of the World: An Online Reference, , http://research.amnh.org/herpetology/amphibia/index.html, American Museum of Natural History, New York, USA, Version 6.0., Electronic Database accessible at
  • Gallardo, J.M., Los anfibios de la Provincia de Entre Ríos, Argentina y algunas notas sobre su distribución geográfica y ecología (1964) Neotropica, 31, pp. 23-38
  • Toledo, L.F., Ribeiro, R.S., Haddad, C.F.B., Anurans as prey: an exploratory analysis and size relationships between predators and their prey (2007) J Zool, 271 (2), pp. 170-177
  • Clarke, B.T., The natural history of amphibian skin secretions, their normal functioning and potential medical applications (1997) Biol Rev, 72 (3), pp. 365-379
  • Sherman, P.J., Jackway, R.J., Nicholson, E., Musgrave, I.F., Boontheung, P., Bowie, J.H., Activities of seasonably variable caerulein and rothein skin peptides from the tree frogs Litoria splendida and Litoria rothii (2009) Toxicon, 54 (6), pp. 828-835
  • Chen, K.K., Jensen, H., Chen, L., The physiological action of the principles isolated from the secretion of Bufo arenarum (1933) JPET, 49, pp. 1-13
  • Rees, R., Schindler, O., Deulofeu, V., Reichstein, T., (1959) Die Bufogenine des Paratoidensekretes von Bufo arenarum Hensel Helvetica, 42, pp. 2400-2418
  • Maciel, N.M., Schwartz, C.A., Rodrigues Pires Júnior, O., Composition of indolealkylamines of Bufo rubescens cutaneous secretions compared to six other Brazilian bufonids with phylogenetic implications (2003) Comp Biochem Physiol B Bioche Mol Biol., 134, pp. 641-649
  • Mebs, D., Wagner, M.G., Pogoda, W., Maneyro, R., Kwet, A., Kauert, G., Lack of bufadienolides in the skin secretion of red bellied toads, Melanophryniscus spp. (Anura, Bufonidae), from Uruguay (2007) Comp Biochem Physiol C Toxicol Pharmacol, 144 (4), pp. 398-402
  • Ferreira, P.M.P., Lima, D.J.B., Debiasi, B.W., Antiproliferative activity of Rhinella marina and Rhaebo guttatus venom extracts from Southern Amazon (2013) Toxicon, 72, pp. 43-51
  • Sciani, J.M., Angeli, C.V., Antoniazzi, M.M., Jared, C., Pimenta, D.C., Differences and similarities among parotoid macrogland secretions in South American toads: a preliminary biochemical delineation (2013) The Scientific World Journal, p. 9. , https://doi.org/10.1155/2013/937407, 9 pages, 2013
  • Rash, L.D., Morales, R.A.V., Vink, S., Alewood, P.F., De novo sequencing of peptides from the parotid secretion of the cane toad, Bufo marinus (Rhinella marina) (2011) Toxicon, 57 (2), pp. 208-216
  • Mailho-Fontana, P.L., Antoniazzi, M.M., Toledo, L.F., Passive and active defense in toads: the parotoid macroglands in Rhinella marina and Rhaebo guttatus (2014) J. Exp. Zool., 321 A, p. 65
  • Kerkhoff, J., Noronha, J., Bonfilio, R., Quantification of bufadienolides in the poisons of Rhinella marina and Rhaebo guttatus by HPLC-UV (2016) Toxicon, 119, pp. 311-318
  • Schmeda-Hirschmann, G., Quispe, C., Arana, G.V., Theoduloz, C., Urra, F.A., Cárdenas, C., Antiproliferative activity and chemical composition of the venom from the Amazonian toad Rhinella marina (Anura: Bufonidae) (2016) Toxicon, 121, pp. 119-129
  • Schmeda-Hirschmann, G., Quispe, C., Theoduloz, C., Teixeira de Sousa, P., Parizotto, C., Antiproliferative activity and new argininyl bufadienolide esters from the “Cururú” toad Rhinella (Bufo) schneideri (2014) J Ethnopharmacol, 155 (2), pp. 1076-1085
  • Sousa-Filho, L.M., Freitas, C.D., Lobo, M.D., Biochemical profile, biological activities, and toxic effects of proteins in the Rhinella schneideri parotoid gland secretion (2016) J Exp Zool A Ecol Genet Physiol, 325 (8), pp. 511-523
  • Nonami, H., Fukui, S., Erra-Balsells, R., β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of flight mass spectrometry of proteins and sulfated oligosaccharides: a comparative study using phenylcarbonyl compounds, carbazoles and classical matrices (1997) J Mass Spectrom, 32 (3), pp. 287-296
  • Cathers, T., Lewbart, G.A., Correa, M., Stevens, J.B., Serum chemistry and hematology values for anesthetized American bullfrogs (Rana catesbeiana) (1997) J Zoo Wildl Med, 28, p. 171

Citas:

---------- APA ----------
Petroselli, G., Raices, M., Jungblut, L.D., Pozzi, A.G. & Erra-Balsells, R. (2018) . MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation. Journal of Mass Spectrometry, 53(6), 465-475.
http://dx.doi.org/10.1002/jms.4082
---------- CHICAGO ----------
Petroselli, G., Raices, M., Jungblut, L.D., Pozzi, A.G., Erra-Balsells, R. "MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation" . Journal of Mass Spectrometry 53, no. 6 (2018) : 465-475.
http://dx.doi.org/10.1002/jms.4082
---------- MLA ----------
Petroselli, G., Raices, M., Jungblut, L.D., Pozzi, A.G., Erra-Balsells, R. "MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation" . Journal of Mass Spectrometry, vol. 53, no. 6, 2018, pp. 465-475.
http://dx.doi.org/10.1002/jms.4082
---------- VANCOUVER ----------
Petroselli, G., Raices, M., Jungblut, L.D., Pozzi, A.G., Erra-Balsells, R. MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation. J. Mass Spectrom. 2018;53(6):465-475.
http://dx.doi.org/10.1002/jms.4082