Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. Copyright © 2011 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
Autor:Mandal, M.K.; Chen, L.C.; Yu, Z.; Nonami, H.; Erra-Balsells, R.; Hiraoka, K.
Filiación:Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan
College of Chemistry and Biology, Shenyang Normal University, Shenyang, China
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan
CIHIDECAR-CONICET, Departamento de Quimica Organica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:detergents; electrospray; multiply charged protein ions; nano-electrospray; probe electrospray ionization; Analytical tool; Biological cells; Charged proteins; Detergent solution; Direct analysis; Electrospray ionization mass spectrometry; Electrosprays; Extraction protocols; nano-electrospray; Nano-ESI; Protein extract; Sample solution; Sodium dodecyl sulphate; Triton X-100; Bromine compounds; Ionization; Mass spectrometry; Probes; Proteins; Soaps (detergents); Tissue; Electrospray ionization; 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid; cetrimonium chloride; detergent; dodecyl sulfate sodium; triton x 100; article; controlled study; electrospray; electrospray mass spectrometry; nanoanalysis; priority journal; protein analysis; Cetrimonium Compounds; Detergents; Equipment Design; Octoxynol; Proteins; Sodium Dodecyl Sulfate; Solutions; Spectrometry, Mass, Electrospray Ionization
Año:2011
Volumen:46
Número:10
Página de inicio:967
Página de fin:975
DOI: http://dx.doi.org/10.1002/jms.1977
Título revista:Journal of Mass Spectrometry
Título revista abreviado:J. Mass Spectrom.
ISSN:10765174
CODEN:JMSPF
CAS:3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid, 75621-03-3; cetrimonium chloride, 112-02-7; dodecyl sulfate sodium, 151-21-3; Cetrimonium Compounds; Detergents; Octoxynol, 9002-93-1; Proteins; Sodium Dodecyl Sulfate, 151-21-3; Solutions; cetrimonium, 6899-10-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v46_n10_p967_Mandal

Referencias:

  • Yao, Y., Hong, S., Zhou, H., Yuan, T., Zeng, R., Liao, K., The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae (2009) Cell Res., 19, pp. 497-506
  • Garavito, R.M., Ferguson-Miller, S., Detergents as Tools in Membrane Biochemistry (2001) J. Biol. Chem., 276, pp. 32403-32406
  • Nomura, E., Katsuta, K., Ueda, T., Toriyama, M., Mori, T., Inagaki, N., Acid-labile surfactant improves in-sodium dodecyl sulfate polyacrylamide gel protein digestion for matrix-assisted laser desorption/ionization mass spectrometric peptide mapping (2004) Journal of Mass Spectrometry, 39 (2), pp. 202-207. , DOI 10.1002/jms.578
  • Palmer, C.P., Polymeric and polymer-supported pseudostationary phases in micellar electrokinetic chromatography: Performance and selectivity (2000) Electrophoresis, 21 (18), pp. 4054-4072
  • Barco, M., Planas, C., Palacios, O., Ventura, F., Rivera, J., Caixach, J., Simultaneous quantitative analysis of anionic, cationic, and nonionic surfactants in water by electrospray ionization mass spectrometry with flow injection analysis (2003) Analytical Chemistry, 75 (19), pp. 5129-5136. , DOI 10.1021/ac020708r
  • Ishihama, Y., Katayama, H., Asakawa, N., Surfactants Usable for Electrospray Ionization Mass Spectrometry (2000) Anal. Biochem., 287, pp. 45-54
  • Weber, K., Osborn, M., The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (1969) J. Biol. Chem., 244, pp. 4406-4412
  • Sharon, M., Ilag, L.L., Robinson, C.V., Evidence for micellar structure in the gas phase (2007) Journal of the American Chemical Society, 129 (28), pp. 8740-8746. , DOI 10.1021/ja067820h
  • Wang, Y., Larsson, D.S.D., Van Der Spoel, D., Encapsulation of myoglobin in a cetyl trimethylammonium bromide micelle in vacuo: A simulation study (2009) Biochemistry, 48, pp. 1006-1015
  • Luche, S., Santoni, V., Rabilloud, T., Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis (2003) Proteomics, 3 (3), pp. 249-253. , DOI 10.1002/pmic.200390037
  • Stark, R.E., Leff, P.D., Milheim, S.G., Kropf, A., Physical studies of CHAPS, a new detergent for the study of visual pigments (1984) J. Phys. Chem., 88, pp. 6063-6067
  • Loo, R.R., Dales, N., Andrews, P.C., Surfactant effects on protein structure examined by electrospray ionization mass spectrometry (1994) Protein Sci., 3, pp. 1975-1983
  • Rundlett, K.L., Armstrong, D.W., Mechanism of Signal Suppression by Anionic Surfactants in Capillary Electrophoresis - Electrospray Ionization Mass Spectrometry (1996) Anal. Chem., 68, pp. 3493-3497
  • Fridriksson, E.K., Baird, B., McLafferty, F.W., Electrospray mass spectra from protein electroeluted from sodium dodecylsulfate polyacrylamide gel electrophoresis gels (1999) Journal of the American Society for Mass Spectrometry, 10 (5), pp. 453-455. , DOI 10.1016/S1044-0305(99)00012-4, PII S1044030599000124
  • Li, F., Dong, M., Miller, L.J., Naylor, S., Efficient removal of sodium dodecyl sulfate (SDS) enhances analysis of proteins by SDS-polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (1999) Rapid Commun. Mass Spectrom., 13, pp. 464-465
  • Guerrier, L., Thulasiraman, V., Castagna, A., Fortis, F., Lin, S., Lomas, L., Righetti, P.G., Boschetti, E., Reducing protein concentration range of biological samples using solid-phase ligand libraries (2006) J. Chromatogr. B., 833, pp. 33-40
  • Strege, M.A., Lagu, A.L., Micellar electrokinetic chromatography of proteins (1997) Journal of Chromatography A, 780 (1-2), pp. 285-296. , DOI 10.1016/S0021-9673(97)00361-0, PII S0021967397003610
  • Zhang, L., Orlando, R., Solid-phase extraction/MALDI-MS: Extended ion-pairing surfaces for the on-target cleanup of protein samples (1999) Anal. Chem., 71, pp. 4753-4757
  • Astorga-Wells, J., Jornvall, H., Bergman, T., A microfluidic electrocapture device in sample preparation for protein analysis by MALDI mass spectrometry (2003) Analytical Chemistry, 75 (19), pp. 5213-5219. , DOI 10.1021/ac0300901
  • Liu, C., Verma, S.S., Direct coupling of ionic high-performance liquid chromatography with electrospray ionization mass spectrometry utilizing a microdialysis junction interface (1999) Journal of Chromatography A, 835 (1-2), pp. 93-104. , DOI 10.1016/S0021-9673(98)01069-3, PII S0021967398010693
  • Cristoni, S., Bernardi, L.R., Development of new methodologies for the mass spectrometry study of bioorganic macromolecules (2003) Mass Spectrom. Rev., 22, pp. 369-406
  • Rey, M., Mrázek, H., Pompach, P., Novák, P., Pelosi, L., Brandolin, G., Forest, E., Man, P., Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics (2010) Anal. Chem., 82, pp. 5107-5116
  • Yeung, Y.-G., Nieves, E., Angeletti, R.H., Stanley, E.R., Removal of detergents from protein digests for mass spectrometry analysis (2008) Anal. Biochem., 382, pp. 135-137
  • Badu-Tawiah, A., Cooks, R.G., Enhanced ion signals in desorption electrospray ionization using surfactant spray solutions (2010) J. Am. Soc. Mass Spectrom., 21, pp. 1423-1431
  • Jackson, A.U., Talaty, N., Cooks, R.G., Van Berkel, G.J., Salt Tolerance of Desorption Electrospray Ionization (DESI) (2007) Journal of the American Society for Mass Spectrometry, 18 (12), pp. 2218-2225. , DOI 10.1016/j.jasms.2007.09.018, PII S1044030507008513
  • Barbula, G.K., Safi, S., Chingin, K., Perry, R.H., Zare, R.N., Interfacing Capillary-Based Separations to Mass Spectrometry Using Desorption Electrospray Ionization (2011) Anal. Chem., 83, pp. 1955-1959
  • Shieh, I.-F., Lee, C.-Y., Shiea, J., Eliminating the interferences from TRIS buffer and SDS in protein analysis by fused-droplet electrospray ionization mass spectrometry (2005) Journal of Proteome Research, 4 (2), pp. 606-612. , DOI 10.1021/pr049765m
  • Hiraoka, K., Nishidate, K., Mori, K., Asakawa, D., Suzuki, S., Development of probe electrospray using a solid needle (2007) Rapid Communications in Mass Spectrometry, 21 (18), pp. 3139-3144. , DOI 10.1002/rcm.3201
  • Chen, L.C., Nishidate, K., Saito, Y., Mori, K., Asakawa, D., Takeda, S., Kubota, T., Hiraoka, K., Characteristics of Probe Electrospray Generated from a Solid Needle (2008) J. Phys. Chem. B, 112, pp. 11164-11170
  • Chen, L.C., Yoshimura, K., Yu, Z., Iwata, R., Ito, H., Suzuki, H., Mori, K., Hiraoka, K., Ambient imaging mass spectrometry by electrospray ionization using solid needle as sampling probe (2009) J. Mass Spectrom., 44, pp. 1469-1477
  • Hong, C.-M., Lee, C.-T., Lee, Y.-M., Kuo, C.-P., Yuan, C.-H., Shiea, J., Generating electrospray from solutions predeposited on a copper wire (1999) Rapid Communications in Mass Spectrometry, 13 (1), pp. 21-25. , DOI 10.1002/(SICI)1097-0231(19990115)13:1<21::AID-RCM441>3.0.CO;2-7
  • Mandal, M.K., Chen, L.C., Hashimoto, Y., Yu, Z., Hiraoka, K., Detection of biomolecules from solutions with high concentration of salts using probe electrospray and nano-electrospray ionization mass spectrometry (2010) Anal. Methods., 2, p. 1905
  • Cech, N.B., Enke, C.G., Practical implications of some recent studies in electrospray ionization fundamentals (2001) Mass Spectrom. Rev., 20, pp. 362-387
  • Puchades, M., Westman, A., Blennow, K., Davidsson, P., Removal of sodium dodecyl sulfate from protein samples prior to matrix-assisted laser desorption/ionization mass spectrometry (1999) Rapid Communications in Mass Spectrometry, 13 (5), pp. 344-349
  • Li, F., Li, G.-Z., Wang, H.-Q., Xue, Q.-J., Studies on cetyltrimethylammonium bromide (CTAB) micellar solution and CTAB reversed microemulsion by ESR and 2H NMR (1997) Colloids and Surfaces A: Physicochemical and Engineering Aspects, 127 (1-3), pp. 89-96. , DOI 10.1016/S0927-7757(96)03889-7, PII S0927775796038897
  • Arnold, T., Linke, D., The use of detergents to purify membrane proteins (2008) Curr. Protoc. Protein Sci., 53, pp. 481-4830
  • Walikonis, R.S., Jensen, O.N., Mann, M., Provance Jr., D.W., Mercer, J.A., Kennedy, M.B., Identification of proteins in the postsynaptic density fraction by mass spectrometry (2000) Journal of Neuroscience, 20 (11), pp. 4069-4080
  • Bodzon-Kulakowska, A., Bierczynska-Krzysik, A., Dylag, T., Drabik, A., Suder, P., Noga, M., Jarzebinska, J., Silberring, J., Methods for samples preparation in proteomic research (2007) Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 849 (1-2), pp. 1-31. , DOI 10.1016/j.jchromb.2006.10.040, PII S1570023206008555
  • Ishihama, Y., Sato, T., Tabata, T., Miyamoto, N., Sagane, K., Nagasu, T., Oda, Y., Quantitative mouse brain proteomics using culture-derived isotope tags as internal standards (2005) Nature Biotechnology, 23 (5), pp. 617-621. , DOI 10.1038/nbt1086
  • Quach, T.T.T., Li, N., Richards, D.P., Zheng, J., Keller, B.O., Li, L., Development and Applications of In-Gel CNBr/Tryptic Digestion Combined with Mass Spectrometry for the Analysis of Membrane Proteins (2003) Journal of Proteome Research, 2 (5), pp. 543-552. , DOI 10.1021/pr0340126
  • Guerrier, L., Righetti, P.G., Boschetti, E., Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library (2008) Nature Protocols, 3 (5), pp. 883-890. , DOI 10.1038/nprot.2008.59, PII NPROT.2008.59

Citas:

---------- APA ----------
Mandal, M.K., Chen, L.C., Yu, Z., Nonami, H., Erra-Balsells, R. & Hiraoka, K. (2011) . Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS). Journal of Mass Spectrometry, 46(10), 967-975.
http://dx.doi.org/10.1002/jms.1977
---------- CHICAGO ----------
Mandal, M.K., Chen, L.C., Yu, Z., Nonami, H., Erra-Balsells, R., Hiraoka, K. "Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)" . Journal of Mass Spectrometry 46, no. 10 (2011) : 967-975.
http://dx.doi.org/10.1002/jms.1977
---------- MLA ----------
Mandal, M.K., Chen, L.C., Yu, Z., Nonami, H., Erra-Balsells, R., Hiraoka, K. "Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)" . Journal of Mass Spectrometry, vol. 46, no. 10, 2011, pp. 967-975.
http://dx.doi.org/10.1002/jms.1977
---------- VANCOUVER ----------
Mandal, M.K., Chen, L.C., Yu, Z., Nonami, H., Erra-Balsells, R., Hiraoka, K. Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS). J. Mass Spectrom. 2011;46(10):967-975.
http://dx.doi.org/10.1002/jms.1977