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The vascular endothelium plays an important role in the regulation of vascular tone, cell growth, inflammation, and thrombogenicity. Endothelium dysfunction, then, is considered to promote several disorders that initiate the atherosclerosis process. Vascular tone dysfunction can be determined by high-resolution ultrasonographic imaging of the brachial artery, enabling one to assess endothelium-dependent flow-mediated dilation (FMD). It is based on the principle that an increase in blood flow, specifically in shear stress, provokes the release of nitric oxide and then a vasodilation that can be quantified. In this study, brachial artery diameter evolution was continuously followed during baseline and hyperemia after forearm occlusion using a custom designed software. Some techniques used to measure FMD are limited by operator dependence. We present a new, automated, and versatile method of FMD quantification based on B-mode echographic images and edge detection algorithms. Edges for each image in the acquired sequences are recognized as interfaces based on the grey-level profiles of the averaged pixel values. Within-reading and within-subject FMD% coefficients of variation were 7% and 10%, respectively. This technique largely improves manual measurements and was shown to be appropriate for wide clinical use. © 2008 Lippincott Williams & Wilkins.


Documento: Artículo
Título:New assessment of endothelium-dependent flow-mediated vasodilation to characterize endothelium dysfunction
Autor:Craiem, D.; Chironi, G.; Simon, A.; Levenson, J.
Filiación:Universidad Favaloro, Facultad de Ciencias Exactes y Naturales, Buenos Aires, Argentina
Hôpital Européen Georges Pompidou (Site Broussais), Centre de Médecine Préventive Cardiovasculaire, Université René Descartes-Faculté de Médecine Paris 5, Paris, France
Centre de Médecine Préventive Cardiovasculaire, Hôpital Broussais, 96 rue Didot, F-75674 Paris, France
Palabras clave:Age; Endothelium; Flow-mediated vasodilation; Ultrasonography; nitric oxide; nitric oxide; adult; article; atherosclerosis; automation; B scan; blood flow; blood vessel diameter; blood vessel tone; brachial angiography; clinical article; clinical assessment; controlled study; diastolic blood pressure; endothelial dysfunction; female; human; hyperemia; image analysis; male; priority journal; vasodilatation; algorithm; blood pressure; brachial artery; computer program; echography; forearm; metabolism; middle aged; observer variation; physiology; reproducibility; vascular endothelium; vascularization; vasodilatation; Adult; Algorithms; Blood Pressure; Brachial Artery; Endothelium, Vascular; Female; Forearm; Humans; Male; Middle Aged; Nitric Oxide; Observer Variation; Regional Blood Flow; Reproducibility of Results; Software; Vasodilation
Página de inicio:340
Página de fin:344
Título revista:American Journal of Therapeutics
Título revista abreviado:Am. J. Ther.
CAS:nitric oxide, 10102-43-9; Nitric Oxide, 10102-43-9


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---------- APA ----------
Craiem, D., Chironi, G., Simon, A. & Levenson, J. (2008) . New assessment of endothelium-dependent flow-mediated vasodilation to characterize endothelium dysfunction. American Journal of Therapeutics, 15(4), 340-344.
---------- CHICAGO ----------
Craiem, D., Chironi, G., Simon, A., Levenson, J. "New assessment of endothelium-dependent flow-mediated vasodilation to characterize endothelium dysfunction" . American Journal of Therapeutics 15, no. 4 (2008) : 340-344.
---------- MLA ----------
Craiem, D., Chironi, G., Simon, A., Levenson, J. "New assessment of endothelium-dependent flow-mediated vasodilation to characterize endothelium dysfunction" . American Journal of Therapeutics, vol. 15, no. 4, 2008, pp. 340-344.
---------- VANCOUVER ----------
Craiem, D., Chironi, G., Simon, A., Levenson, J. New assessment of endothelium-dependent flow-mediated vasodilation to characterize endothelium dysfunction. Am. J. Ther. 2008;15(4):340-344.