Artículo

Giudicessi, S.L.; Salum, M.L.; Saavedra, S.L.; Martínez-Ceron, M.C.; Cascone, O.; Erra-Balsells, R.; Camperi, S.A. "Simple method to assess stability of immobilized peptide ligands against proteases" (2017) Journal of Peptide Science. 23(9):685-692
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Abstract:

Although peptides are used as affinity chromatography ligands, they could be digested by proteases. Usually, peptide stability is evaluated in solution, which differs from the resin-bounded peptide behavior. Furthermore, the study of the degradation products requires purification steps before analysis. Here, we describe an easy method to assess immobilized peptide stability. Sample peptides were synthesized on hydroxymethylbenzamide-ChemMatrix resin. Peptidyl-resin beads were then incubated with solutions containing proteases. Peptides were detached from the solid support with ammonia vapor and analyzed by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry, allowing the detection of the whole peptides as well as their C-terminal degradation products. The method allowed a fast evaluation of peptide ligand stability in solid phase towards proteases that may be present in the crude sample before their use as ligands in affinity chromatography. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Simple method to assess stability of immobilized peptide ligands against proteases
Autor:Giudicessi, S.L.; Salum, M.L.; Saavedra, S.L.; Martínez-Ceron, M.C.; Cascone, O.; Erra-Balsells, R.; Camperi, S.A.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Junín 956, Buenos Aires, 1113, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Nanobiotecnología (NANOBIOTEC), Junín 956, Buenos Aires, 1113, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica. Pabellón II, Ciudad Universitaria, Intendente Güiraldes 2160, Buenos Aires, 1428, Argentina
CONICET-Universidad de Buenos Aires, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Pabellón II, Ciudad Universitaria, Intendente Güiraldes 2160, Buenos Aires, 1428, Argentina
Palabras clave:affinity chromatography; ESI MS; MALDI MS; purification; ammonia; benzamide derivative; immobilized protein; ligand; proteinase; resin; peptide; peptide hydrolase; affinity chromatography; analytic method; Article; carboxy terminal sequence; matrix-assisted laser desorption-ionization mass spectrometry; peptide synthesis; priority journal; protein degradation; solid; chemistry; metabolism; Chromatography, Affinity; Peptide Hydrolases; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Año:2017
Volumen:23
Número:9
Página de inicio:685
Página de fin:692
DOI: http://dx.doi.org/10.1002/psc.3012
Título revista:Journal of Peptide Science
Título revista abreviado:J. Pept. Sci.
ISSN:10752617
CODEN:JPSIE
CAS:ammonia, 14798-03-9, 51847-23-5, 7664-41-7; proteinase, 9001-92-7; peptide hydrolase; Peptide Hydrolases; Peptides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10752617_v23_n9_p685_Giudicessi

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Citas:

---------- APA ----------
Giudicessi, S.L., Salum, M.L., Saavedra, S.L., Martínez-Ceron, M.C., Cascone, O., Erra-Balsells, R. & Camperi, S.A. (2017) . Simple method to assess stability of immobilized peptide ligands against proteases. Journal of Peptide Science, 23(9), 685-692.
http://dx.doi.org/10.1002/psc.3012
---------- CHICAGO ----------
Giudicessi, S.L., Salum, M.L., Saavedra, S.L., Martínez-Ceron, M.C., Cascone, O., Erra-Balsells, R., et al. "Simple method to assess stability of immobilized peptide ligands against proteases" . Journal of Peptide Science 23, no. 9 (2017) : 685-692.
http://dx.doi.org/10.1002/psc.3012
---------- MLA ----------
Giudicessi, S.L., Salum, M.L., Saavedra, S.L., Martínez-Ceron, M.C., Cascone, O., Erra-Balsells, R., et al. "Simple method to assess stability of immobilized peptide ligands against proteases" . Journal of Peptide Science, vol. 23, no. 9, 2017, pp. 685-692.
http://dx.doi.org/10.1002/psc.3012
---------- VANCOUVER ----------
Giudicessi, S.L., Salum, M.L., Saavedra, S.L., Martínez-Ceron, M.C., Cascone, O., Erra-Balsells, R., et al. Simple method to assess stability of immobilized peptide ligands against proteases. J. Pept. Sci. 2017;23(9):685-692.
http://dx.doi.org/10.1002/psc.3012