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Abstract:

The isoforms of cAMP-dependent protein kinase (PKA) show distinct biochemical properties and subcellular localization, suggesting different physiological functions, and conferring the fine-tuning between the activation of cAMP-PKA cascade and the cellular response. The critical role of PKA in memory and synaptic plasticity has been extensively demonstrated both in vertebrates and invertebrates, but the role of PKA isoforms is a matter of debate. Here we present experimental data showing differential PKA activation profiles after two different experiences: an instance of associative contextual learning (context-signal learning) and a single exposure to a novel context, both in the learning and memory model of the crab Chasmagnathus. Differences were found in the temporal course of activation and in the involvement of PKA isoforms. We found increased PKA activity immediately and 6 h after context-signal training correlating with the critical periods during which pharmacological inhibition of PKA disrupts memory formation. In contrast, PKA activity increased immediately but not 6 h after single exposure to a novel context. The amounts of PKA I and PKA II holoenzymes were analyzed to determine changes in holoenzyme levels and/or differential activation induced by both experiences. Results indicate that context-induced PKA activation is at least in part due to PKA II, and that PKA activation 6 h after context-signal learning coincides with an increase in the total level of PKA I. Considering the higher sensitivity of PKA I to cAMP, its increment can account for the PKA activation found 6 h after training and is proposed as a novel mechanism providing the prolonged PKA activation during memory consolidation. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Differential activity profile of cAMP-dependent protein kinase isoforms during long-term memory consolidation in the crab Chasmagnathus
Autor:Locatelli, F.; Romano, A.
Filiación:Lab. de Neurbio. de la Memoria, Depto. Fisiol., Biol. Molec. Y Cel., Pab II, (1428) Buenos Aires, Argentina
Institut für Biologie, Freie Universität Berlin, Neurobiologie, Königin-Luise-Strasse 28/30, D-14195 Berlin, Germany
Palabras clave:cAMP pathway; Crab; Invertebrates; Long-term memory; PKA isoforms; cyclic AMP; holoenzyme; isoprotein; protein kinase; animal experiment; article; crab; enzyme activation; enzyme activity; enzyme inhibition; experience; long term memory; male; memory consolidation; nonhuman; signal transduction; state dependent learning; training
Año:2005
Volumen:83
Número:3
Página de inicio:232
Página de fin:242
DOI: http://dx.doi.org/10.1016/j.nlm.2005.01.002
Título revista:Neurobiology of Learning and Memory
Título revista abreviado:Neurobiol. Learn. Mem.
ISSN:10747427
CODEN:NLMEF
CAS:cyclic AMP, 60-92-4; protein kinase, 9026-43-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10747427_v83_n3_p232_Locatelli

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Citas:

---------- APA ----------
Locatelli, F. & Romano, A. (2005) . Differential activity profile of cAMP-dependent protein kinase isoforms during long-term memory consolidation in the crab Chasmagnathus. Neurobiology of Learning and Memory, 83(3), 232-242.
http://dx.doi.org/10.1016/j.nlm.2005.01.002
---------- CHICAGO ----------
Locatelli, F., Romano, A. "Differential activity profile of cAMP-dependent protein kinase isoforms during long-term memory consolidation in the crab Chasmagnathus" . Neurobiology of Learning and Memory 83, no. 3 (2005) : 232-242.
http://dx.doi.org/10.1016/j.nlm.2005.01.002
---------- MLA ----------
Locatelli, F., Romano, A. "Differential activity profile of cAMP-dependent protein kinase isoforms during long-term memory consolidation in the crab Chasmagnathus" . Neurobiology of Learning and Memory, vol. 83, no. 3, 2005, pp. 232-242.
http://dx.doi.org/10.1016/j.nlm.2005.01.002
---------- VANCOUVER ----------
Locatelli, F., Romano, A. Differential activity profile of cAMP-dependent protein kinase isoforms during long-term memory consolidation in the crab Chasmagnathus. Neurobiol. Learn. Mem. 2005;83(3):232-242.
http://dx.doi.org/10.1016/j.nlm.2005.01.002