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Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-kB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-kB was activated 45. min after training in a novel object recognition (NOR) task and that inhibiting NF-kB immediately after training by intrahippocampal administration of NF-kB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45. min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-kB by NF-kB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-kB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-kB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation. © 2015 Elsevier Inc.


Documento: Artículo
Título:Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
Autor:Zalcman, G.; Federman, N.; de la Fuente, V.; Romano, A.
Filiación:Laboratorio de Neurobiologia de la Memoria, Departamento de Fisiologia, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIByNE, CONICET, Ciudad Universitaria, Pab. II, 2do piso, EHA Buenos Aires, 1428, Argentina
Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), CONICET-Partner Inst. of the Max Planck Society, Polo Científico Tecnológico, 3er piso, Buenos Aires, Argentina
Palabras clave:Memory consolidation; NF-κB; Transcription factors; Zif268; complementary DNA; immunoglobulin enhancer binding protein; messenger RNA; oligonucleotide; transcription factor; transcription factor Zif268; unclassified drug; early growth response factor 1; Egr1 protein, mouse; immunoglobulin enhancer binding protein; animal experiment; animal tissue; Article; controlled study; gene expression regulation; gene function; hippocampus; immediate early gene; long term memory; male; memory consolidation; mental task; mouse; nonhuman; pattern recognition; protein expression; recognition; task performance; transcription regulation; Zif268 gene; animal; gene expression regulation; metabolism; physiology; recognition; signal transduction; Animals; Early Growth Response Protein 1; Gene Expression Regulation; Hippocampus; Male; Mice; NF-kappa B; Recognition (Psychology); Signal Transduction
Página de inicio:10
Página de fin:17
Título revista:Neurobiology of Learning and Memory
Título revista abreviado:Neurobiol. Learn. Mem.
CAS:Early Growth Response Protein 1; Egr1 protein, mouse; NF-kappa B


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---------- APA ----------
Zalcman, G., Federman, N., de la Fuente, V. & Romano, A. (2015) . Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice. Neurobiology of Learning and Memory, 119, 10-17.
---------- CHICAGO ----------
Zalcman, G., Federman, N., de la Fuente, V., Romano, A. "Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice" . Neurobiology of Learning and Memory 119 (2015) : 10-17.
---------- MLA ----------
Zalcman, G., Federman, N., de la Fuente, V., Romano, A. "Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice" . Neurobiology of Learning and Memory, vol. 119, 2015, pp. 10-17.
---------- VANCOUVER ----------
Zalcman, G., Federman, N., de la Fuente, V., Romano, A. Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice. Neurobiol. Learn. Mem. 2015;119:10-17.