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Abstract:

Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production system, E7 HPV type 16 protein, an attractive candidate for anticancer vaccine development, was engineered to be expressed in tobacco chloroplast. In addition, E7 coding sequence was fused to potato virus X coat protein (CP) to compare expression level. Results show that E7CP transcript accumulation reached lower levels than non-fused E7. However, antigen expression levels were higher for fusion protein indicating that CP stabilizes E7 peptide in the chloroplast stroma. These results support viability of transplastomic plants for antigen production and the relevance of improving recombinant peptide stability for certain transgenes to enhance protein accumulation in this organelle. © 2009 Humana Press.

Registro:

Documento: Artículo
Título:Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast
Autor:Morgenfeld, M.; Segretin, M.E.; Wirth, S.; Lentz, E.; Zelada, A.; Mentaberry, A.; Gissmann, L.; Bravo-Almonacid, F.
Filiación:Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, C.C. 1428 Ciudad Autónoma de Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular (FCEN-UBA), Intendente Güiraldes 2620 Pab. II, C.I. 428EHA Ciudad Universitaria, Buenos Aires, Argentina
Department of Genome Modifications and Carcinogenesis, German Cancer Research Center, Heidelberg 69120, Germany
Palabras clave:Chloroplast transformation; E7 antigen; Fusion protein; Human papillomavirus (HPV); Molecular farming; Transplastomic tobacco; Chloroplast transformation; E7 antigen; Fusion protein; Human papillomavirus (HPV); Molecular farming; Transplastomic tobacco; Amines; Chlorophyll; Peptides; System stability; Tobacco; Antigens; coat protein; protein E7; article; chloroplast; gene sequence; nonhuman; nucleotide sequence; polymerase chain reaction; protein engineering; protein expression; protein stability; Southern blotting; stroma cell; tobacco; Blotting, Northern; Blotting, Southern; Capsid Proteins; Chloroplasts; Cloning, Molecular; Oncogene Proteins, Viral; Plants, Genetically Modified; Polymerase Chain Reaction; Protein Stability; Recombinant Fusion Proteins; Tobacco; Human papillomavirus; Human papillomavirus - 16; Human papillomavirus type 16; Nicotiana tabacum; Potato virus X
Año:2009
Volumen:43
Número:3
Página de inicio:243
Página de fin:249
DOI: http://dx.doi.org/10.1007/s12033-009-9195-3
Título revista:Molecular Biotechnology
Título revista abreviado:Mol. Biotechnol.
ISSN:10736085
CAS:Capsid Proteins; Oncogene Proteins, Viral; Recombinant Fusion Proteins; coat protein, Potato virus X; oncogene protein E7, Human papillomavirus type 16
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10736085_v43_n3_p243_Morgenfeld

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Citas:

---------- APA ----------
Morgenfeld, M., Segretin, M.E., Wirth, S., Lentz, E., Zelada, A., Mentaberry, A., Gissmann, L.,..., Bravo-Almonacid, F. (2009) . Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast. Molecular Biotechnology, 43(3), 243-249.
http://dx.doi.org/10.1007/s12033-009-9195-3
---------- CHICAGO ----------
Morgenfeld, M., Segretin, M.E., Wirth, S., Lentz, E., Zelada, A., Mentaberry, A., et al. "Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast" . Molecular Biotechnology 43, no. 3 (2009) : 243-249.
http://dx.doi.org/10.1007/s12033-009-9195-3
---------- MLA ----------
Morgenfeld, M., Segretin, M.E., Wirth, S., Lentz, E., Zelada, A., Mentaberry, A., et al. "Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast" . Molecular Biotechnology, vol. 43, no. 3, 2009, pp. 243-249.
http://dx.doi.org/10.1007/s12033-009-9195-3
---------- VANCOUVER ----------
Morgenfeld, M., Segretin, M.E., Wirth, S., Lentz, E., Zelada, A., Mentaberry, A., et al. Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast. Mol. Biotechnol. 2009;43(3):243-249.
http://dx.doi.org/10.1007/s12033-009-9195-3