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Rosmarinus officinalis extracts were investigated by a combination of bioassays and biochemical analysis to identify bioactive compounds. The 2,2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging method, Folin-Ciocaulteau method and HPLC chromatography were used to study the distribution and levels of antioxidants (AOXs). Antimicrobial activity analysis was carried out using the disk diffusion and broth dilution techniques. A good correlation between the AOX activities and total phenol content in the extracts was found. Although all rosemary extracts showed a high radical scavenging activity, a different efficacy as antimicrobial agent was observed. Methanol extract containing 30% of carnosic acid, 16% of carnosol and 5% of rosmarinic acid was the most effective antimicrobial against Gram positive bacteria (minimal inhibition concentration, MIC, between 2 and 15 μg/ml), Gram negative bacteria (MIC between 2 and 60 μg/ml) and yeast (MIC of 4 μg/ml). By contrast, water extract containing only 15% of rosmarinic acid showed a narrow activity. MIC value of the methanol and water extracts is in a good correlation with the values obtained with pure carnosic acid and rosmarinic acid, respectively. Therefore, our results suggested that the antimicrobial rosemary extracts efficacy was associated with their specific phenolic composition. Carnosic acid and rosmarinic acid may be the main bioactive antimicrobial compounds present in rosemary extracts. From a practical point of view, rosemary extract may be a good candidate for functional foods as well as for pharmaceutical plant-based products. © 2006 Taylor & Francis.


Documento: Artículo
Título:Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition
Autor:Moreno, S.; Scheyer, T.; Romano, C.S.; Vojnov, A.A.
Filiación:Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Bioquímicas, Universidad de Buenos Aires, Patricias Argentinas 435, Buenos Aires 1405, Argentina
Palabras clave:Antimicrobial activity; Antioxidants; Carnosic acid; Rosmarinic acid; Rosmarinus officinalis; carnosic acid; carnosol; rosmarinic acid; Rosmarinus officinalis extract; scavenger; antimicrobial activity; antioxidant activity; article; chemical composition; controlled study; correlation analysis; drug mechanism; drug screening; drug synthesis; high performance liquid chromatography; minimum inhibitory concentration; nonhuman; rosemary; statistical analysis; statistical significance; Anti-Infective Agents; Antioxidants; Bacteria; Chromatography, High Pressure Liquid; Cinnamates; Depsides; Diterpenes, Abietane; Flavonoids; Free Radicals; Indicators and Reagents; Phenanthrenes; Phenols; Picrates; Plant Extracts; Rosmarinus; Saccharomyces cerevisiae; Negibacteria; Posibacteria; Rosmarinus officinalis
Página de inicio:223
Página de fin:231
Título revista:Free Radical Research
Título revista abreviado:Free Radic. Res.
CAS:carnosol, 5957-80-2; rosmarinic acid, 20283-92-5; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Anti-Infective Agents; Antioxidants; carnosol, 5957-80-2; Cinnamates; Depsides; Diterpenes, Abietane; Flavonoids; Free Radicals; Indicators and Reagents; Phenanthrenes; Phenols; Picrates; Plant Extracts; polyphenols; rosmarinic acid, 537-15-5; salvin, 3650-09-7


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---------- APA ----------
Moreno, S., Scheyer, T., Romano, C.S. & Vojnov, A.A. (2006) . Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Research, 40(2), 223-231.
---------- CHICAGO ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. "Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition" . Free Radical Research 40, no. 2 (2006) : 223-231.
---------- MLA ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. "Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition" . Free Radical Research, vol. 40, no. 2, 2006, pp. 223-231.
---------- VANCOUVER ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radic. Res. 2006;40(2):223-231.