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Abstract:

Rosmarinus officinalis extracts were investigated by a combination of bioassays and biochemical analysis to identify bioactive compounds. The 2,2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging method, Folin-Ciocaulteau method and HPLC chromatography were used to study the distribution and levels of antioxidants (AOXs). Antimicrobial activity analysis was carried out using the disk diffusion and broth dilution techniques. A good correlation between the AOX activities and total phenol content in the extracts was found. Although all rosemary extracts showed a high radical scavenging activity, a different efficacy as antimicrobial agent was observed. Methanol extract containing 30% of carnosic acid, 16% of carnosol and 5% of rosmarinic acid was the most effective antimicrobial against Gram positive bacteria (minimal inhibition concentration, MIC, between 2 and 15 μg/ml), Gram negative bacteria (MIC between 2 and 60 μg/ml) and yeast (MIC of 4 μg/ml). By contrast, water extract containing only 15% of rosmarinic acid showed a narrow activity. MIC value of the methanol and water extracts is in a good correlation with the values obtained with pure carnosic acid and rosmarinic acid, respectively. Therefore, our results suggested that the antimicrobial rosemary extracts efficacy was associated with their specific phenolic composition. Carnosic acid and rosmarinic acid may be the main bioactive antimicrobial compounds present in rosemary extracts. From a practical point of view, rosemary extract may be a good candidate for functional foods as well as for pharmaceutical plant-based products. © 2006 Taylor & Francis.

Registro:

Documento: Artículo
Título:Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition
Autor:Moreno, S.; Scheyer, T.; Romano, C.S.; Vojnov, A.A.
Filiación:Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Bioquímicas, Universidad de Buenos Aires, Patricias Argentinas 435, Buenos Aires 1405, Argentina
Palabras clave:Antimicrobial activity; Antioxidants; Carnosic acid; Rosmarinic acid; Rosmarinus officinalis; carnosic acid; carnosol; rosmarinic acid; Rosmarinus officinalis extract; scavenger; antimicrobial activity; antioxidant activity; article; chemical composition; controlled study; correlation analysis; drug mechanism; drug screening; drug synthesis; high performance liquid chromatography; minimum inhibitory concentration; nonhuman; rosemary; statistical analysis; statistical significance; Anti-Infective Agents; Antioxidants; Bacteria; Chromatography, High Pressure Liquid; Cinnamates; Depsides; Diterpenes, Abietane; Flavonoids; Free Radicals; Indicators and Reagents; Phenanthrenes; Phenols; Picrates; Plant Extracts; Rosmarinus; Saccharomyces cerevisiae; Negibacteria; Posibacteria; Rosmarinus officinalis
Año:2006
Volumen:40
Número:2
Página de inicio:223
Página de fin:231
DOI: http://dx.doi.org/10.1080/10715760500473834
Título revista:Free Radical Research
Título revista abreviado:Free Radic. Res.
ISSN:10715762
CODEN:FRARE
CAS:carnosol, 5957-80-2; rosmarinic acid, 20283-92-5; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Anti-Infective Agents; Antioxidants; carnosol, 5957-80-2; Cinnamates; Depsides; Diterpenes, Abietane; Flavonoids; Free Radicals; Indicators and Reagents; Phenanthrenes; Phenols; Picrates; Plant Extracts; polyphenols; rosmarinic acid, 537-15-5; salvin, 3650-09-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10715762_v40_n2_p223_Moreno

Referencias:

  • Ames, B.N., Shigenaga, M.K., Hagen, T.M., Oxidants, AOXs and the generative disease of aging (1993) Proc Natl Acad Sci USA, 90, pp. 7915-7922
  • Storz, P., Reactive oxygen species in tumor progression (2005) Front Biosci, 10, pp. 1881-1896
  • Rice-Evans, C.A., Miller, N.J., Paganga, G., Antioxidant properties of phenolic compounds (1997) Trends Plant Sci, 2, pp. 152-159
  • Couladis, M., Tzakou, O., Verykokidou, E., Screening of some Greek aromatic plants for antioxidant activity (2003) J Phytother Res, 17, pp. 194-196
  • Prior, R.L., Cao, G.H., Martin, A., Sofic, E., Mcewen, J., Obrien, C., Lischner, N., Mainland, C.M., Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species (1998) J Agric Food Chem, 46, pp. 2686-2693
  • Singletary, K.W., Nelshoppen, J.M., Inhibition of 7,12-dimethylbenzanthracene-(DMBA) induced mammary tumorgenesis and have in vivo formation of mammary DMBA-DNA adducts by rosemary extract (1991) Cancer Lett, 60, pp. 169-175
  • Huang, M.T., Ho, C.T., Wang, Z.Y., Ferraro, T., Lou, Y.R., Stauber, K., Ma, W., Conney, A.H., Inhibition of skin tumorigenesis by rosemary and its constituents carnosol and ursolic acid (1994) Cancer Res, 54, pp. 701-708
  • al-Sereiti, M.R., Abu-Amer, K.M., Sen, P., Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials (1999) Indian J Exp Biol, 37, p. 130
  • Osakabe, N., Yasuda, A., Natsume, M., Yoshikawa, T., Rosmarinic acid inhibits epidermal inflammatory responses: Anticarcinogenic effect of perilla frutescens extract in the murine two-stage skin model (2004) Carcinogenesis, 25, pp. 549-557
  • Petersen, M., Simmonds, M.S.J., Rosmarinic acid (2003) Phytochemistry, 62, pp. 121-125
  • Ono, K., Hasegawa, K., Naiki, H., Yamada, M., Curcumin has potent anti-amyloidogenic effects for Alzheimers β-amyloid fibrils in vitro (2004) J Neurosci Res, 75, pp. 742-750
  • Danilenko, M., Studzinski, G.P., Enhancement by other compounds of the anti-cancer activity of vitamin D (3) and its analogs (2004) Exp Cell Res, 298, pp. 339-358
  • Masuda, T., Inaba, Y., Maekawa, T., Takeda, Y., Tamura, H., Yamaguchi, H., Recovery mechanism of the antioxidant activity from carnosic acid quinine, an oxidized sage and rosemary antioxidant (2002) J Agric Food Chem, 50, pp. 5863-5869
  • Santoyo, S., Cavero, S., Jaime, L., Ibanez, E., Senorans, F.J., Reglero, G., Chemical composition and antimicrobial activity of Rosmarinus officinalis L. essential oil obtained via supercritical fluid extraction (2005) J Food Prot, 68, pp. 790-795
  • Kulisic, T., Radonic, A., Katalinic, V., Milos, M., Use of different methods for testing antioxidative activity of oregano essential oil (2004) Food Chem, 85, pp. 633-640
  • Dorman, H.J.D., Peltoketo, A., Hiltunen, R., Tikkanen, M.J., Characterization of the antioxidant properties of de-odorized aqueous extracts from selected Lamiaceae herbs (2003) Food Chem, 83, pp. 255-262
  • Blois, M.S., Antioxidant determinations by the use of a stable free radical (1958) Nature, 181, pp. 1199-1200
  • Brand-Williams, W., Cuvelier, M.E., Berset, C., Use of a free radical method to evaluate antioxidant activity (1995) Lebensm - Wiss Technol, 28, pp. 25-30
  • Yen, G.C., Duh, P.D., Scavenging effect of methanol rosemary extracts of peanut hulls on free-radical and active-oxygen species (1994) J Agric Food Chem, 42, pp. 629-632
  • Tepe, B., Daferera, D., Sokmen, A., Sokmen, M., Polissiou, M., Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae) (2005) Food Chem, 90, pp. 333-340
  • Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M., Antibiotic susceptibility testing by a standardized single disk method (1966) Am J Clin Pathol, 45, pp. 493-496
  • Bartolomé, B., Bengoechea, M.L., Gálvez, M.C., Pérez-Iizarbe, F.J., Hernández, T., Estrella, I., Gómez-Cordovés, C., Photo-diode array detection for elucidation of the structure of phenolic compounds (1993) J Chromatogr A, 655, pp. 119-125
  • Munne-Bosch, S., Schwarz, K., Alegre, L., Response of abietane diterpenes to stress in Rosmarinus officinalis L.: New insights into the function of diterpenes in plants (1999) Free Radical Res, 31, pp. S107-S112
  • Munne-Bosch, S., Alegre, L., Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants (2000) Planta, 210, pp. 925-931
  • Sacchetti, G., Maietti, S., Muzzoli, M., Scaglianti, M., Manfredini, S., Radice, M., Bruni, R., Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods (2005) Food Chem, 91, pp. 621-632
  • Oluwatuy, M., Katz, G.W., Gibbons, S., Antibacterial and resistance modifying activity of Rosmarinus officinalis (2004) Phytochem, 65, pp. 3249-3254
  • Plouzek, C.A., Ciolino, H.P., Clarke, R., Yeh, G.C., Inhibition of P-glycoprotein activity and reversal of multidrug resistance in vitro rosemary extract (1999) Eur J Cancer, 35, pp. 1541-1545
  • Nutricional and pharmacological effects of food phenolics (2004) Phenolics in Food and Nutraceuticals, pp. 331-402. , New York: CRC Press LLC
  • Cowan, M.M., Plant products as antimicrobial agents (1999) Clin Microbiol Rev, 12, pp. 564-582
  • Lemonica, I.P., Demasceno, D.C., Di-Stasi, L.C., Study of the embryonic effects of an extract of rosemary (Rosmarinus officinalis L.) (1996) Braz J Med Biol Res, 29, pp. 223-227
  • Minnunni, M., Wolleb, U., Mueller, O., Pfeifer, A., Aeschbacher, H.U., Natural antioxidants as inhibitors of oxygen species induced mutagenicity (1992) Mutat Res, 269, pp. 193-200
  • Ozcan, M., Antioxidant activity of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil (2003) J Med Food, 6, pp. 267-270

Citas:

---------- APA ----------
Moreno, S., Scheyer, T., Romano, C.S. & Vojnov, A.A. (2006) . Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Research, 40(2), 223-231.
http://dx.doi.org/10.1080/10715760500473834
---------- CHICAGO ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. "Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition" . Free Radical Research 40, no. 2 (2006) : 223-231.
http://dx.doi.org/10.1080/10715760500473834
---------- MLA ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. "Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition" . Free Radical Research, vol. 40, no. 2, 2006, pp. 223-231.
http://dx.doi.org/10.1080/10715760500473834
---------- VANCOUVER ----------
Moreno, S., Scheyer, T., Romano, C.S., Vojnov, A.A. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radic. Res. 2006;40(2):223-231.
http://dx.doi.org/10.1080/10715760500473834