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The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase was decreased 3 h after paraquat injection. Heme oxygenase-1 induction started 9 h after treatment, peaking at 15 h. δ-aminolevulinic acid synthase induction occurred once heme oxygenase had been enhanced, reaching its maximum (1.5-fold of control) at 16 h. δ-aminolevulinic acid dehydratase activity was 40% inhibited at 3 h showing a profile similar to that of GSH, while porphobilinogenase activity was not modified along the whole period of the assay. Administration of α-tocopherol (35 mmol/kg body weight) 2 h before paraquat treatment entirely prevented the increase in thiobarbituric acid reactive substances (TBARS) content, the decrease in GSH levels as well as heme oxygenase-1 and δ-aminolevulinic acid synthase induction. This study shows that oxidative stress produced by paraquat leads to an increase in δ-aminolevulinic acid synthase and heme oxygenase-1 activities, indicating that the herbicide affects both heme biosynthesis and degradation.


Documento: Artículo
Título:Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase
Autor:Noriega, G.O.; Gonzales, S.; Tomaro, M.L.; Del, A.M.; Batlle, C.
Filiación:Centro de Investigaciones Sobre Porfirinas y Porfirias (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Farmacia y Bioquímica, Departamento de Química Biológica, Universidad de Buenos, Junín 956, Buenos Aires -1113, Argentina
Palabras clave:δ-aminolevulinic acid synthase; Antioxidant enzymes; Heme metabolism; Heme oxygenase-1; Oxidative stress; Paraquat; 5 aminolevulinate synthase; alpha tocopherol; antioxidant; catalase; glutathione; glutathione peroxidase; heme; heme oxygenase 1; herbicide; lipid; liver enzyme; paraquat; porphobilinogen synthase; superoxide dismutase; thiobarbituric acid reactive substance; article; biosynthesis; body weight; controlled study; degradation; enzyme activity; enzyme assay; enzyme inhibition; in vivo study; lipid peroxidation; liver metabolism; nonhuman; oxidative stress; porphyrin metabolism; rat; 5-Aminolevulinate Synthetase; alpha-Tocopherol; Animals; Antioxidants; Enzymes; Female; Glutathione; Heme; Heme Oxygenase (Decyclizing); Heme Oxygenase-1; Herbicides; Liver; Oxidative Stress; Paraquat; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances
Página de inicio:633
Página de fin:639
Título revista:Free Radical Research
Título revista abreviado:Free Radic. Res.
CAS:5-Aminolevulinate Synthetase, EC; alpha-Tocopherol, 59-02-9; Antioxidants; Enzymes; Glutathione, 70-18-8; Heme Oxygenase (Decyclizing), EC; Heme Oxygenase-1, EC; Heme, 14875-96-8; Herbicides; Paraquat, 4685-14-7; Thiobarbituric Acid Reactive Substances


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---------- APA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M. & Batlle, C. (2002) . Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radical Research, 36(6), 633-639.
---------- CHICAGO ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. "Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase" . Free Radical Research 36, no. 6 (2002) : 633-639.
---------- MLA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. "Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase" . Free Radical Research, vol. 36, no. 6, 2002, pp. 633-639.
---------- VANCOUVER ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radic. Res. 2002;36(6):633-639.