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Abstract:

The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase was decreased 3 h after paraquat injection. Heme oxygenase-1 induction started 9 h after treatment, peaking at 15 h. δ-aminolevulinic acid synthase induction occurred once heme oxygenase had been enhanced, reaching its maximum (1.5-fold of control) at 16 h. δ-aminolevulinic acid dehydratase activity was 40% inhibited at 3 h showing a profile similar to that of GSH, while porphobilinogenase activity was not modified along the whole period of the assay. Administration of α-tocopherol (35 mmol/kg body weight) 2 h before paraquat treatment entirely prevented the increase in thiobarbituric acid reactive substances (TBARS) content, the decrease in GSH levels as well as heme oxygenase-1 and δ-aminolevulinic acid synthase induction. This study shows that oxidative stress produced by paraquat leads to an increase in δ-aminolevulinic acid synthase and heme oxygenase-1 activities, indicating that the herbicide affects both heme biosynthesis and degradation.

Registro:

Documento: Artículo
Título:Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase
Autor:Noriega, G.O.; Gonzales, S.; Tomaro, M.L.; Del, A.M.; Batlle, C.
Filiación:Centro de Investigaciones Sobre Porfirinas y Porfirias (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Farmacia y Bioquímica, Departamento de Química Biológica, Universidad de Buenos, Junín 956, Buenos Aires -1113, Argentina
Palabras clave:δ-aminolevulinic acid synthase; Antioxidant enzymes; Heme metabolism; Heme oxygenase-1; Oxidative stress; Paraquat; 5 aminolevulinate synthase; alpha tocopherol; antioxidant; catalase; glutathione; glutathione peroxidase; heme; heme oxygenase 1; herbicide; lipid; liver enzyme; paraquat; porphobilinogen synthase; superoxide dismutase; thiobarbituric acid reactive substance; article; biosynthesis; body weight; controlled study; degradation; enzyme activity; enzyme assay; enzyme inhibition; in vivo study; lipid peroxidation; liver metabolism; nonhuman; oxidative stress; porphyrin metabolism; rat; 5-Aminolevulinate Synthetase; alpha-Tocopherol; Animals; Antioxidants; Enzymes; Female; Glutathione; Heme; Heme Oxygenase (Decyclizing); Heme Oxygenase-1; Herbicides; Liver; Oxidative Stress; Paraquat; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances
Año:2002
Volumen:36
Número:6
Página de inicio:633
Página de fin:639
DOI: http://dx.doi.org/10.1080/10715760290029065
Título revista:Free Radical Research
Título revista abreviado:Free Radic. Res.
ISSN:10715762
CODEN:FRARE
CAS:5-Aminolevulinate Synthetase, EC 2.3.1.37; alpha-Tocopherol, 59-02-9; Antioxidants; Enzymes; Glutathione, 70-18-8; Heme Oxygenase (Decyclizing), EC 1.14.99.3; Heme Oxygenase-1, EC 1.14.99.3; Heme, 14875-96-8; Herbicides; Paraquat, 4685-14-7; Thiobarbituric Acid Reactive Substances
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10715762_v36_n6_p633_Noriega

Referencias:

  • Ali, S., Diwakarand, G., Pawa, S., "Paraquat induces different pulmonary biochemical responses in Wistar rats and Swiss mice" (2000) Chem. Biol. Interact, 125 (2), pp. 79-91
  • Batlle, A., "Porfirias y Porfirinas Aspectos clínicos, bioquímicos y biología molecular Actualizaciones Médico Bioquímicas" (1997) Bioquím. Clín. Latinoamericana, (SUPPL. 3), pp. 1-35
  • Maines, M.D., "Heme oxygenase: Function, multiplicity, regulatory mechanisms and clinical applications" (1988) FASEB. J, 2, pp. 2557-2568
  • Stocker, R., Yamamoto, Y., Mc Donagh, A.F., Glazer, A.N., Ames, B.N., "Bilirubin is an antioxidant of possible physiological importance" (1987) Science, 235, pp. 1043-1046
  • Keyse, S.M., Tyrrell, R.M., "Both near ultraviolet radiation and the oxidizing agent hydrogen peroxide induce a 32-kDa stress protein in normal human skin fibroblasts" (1987) J. Biol. Chem, 262, pp. 14821-14825
  • Stocker, R., "Induction of haem oxygenase as a defence against oxidative stress" (1990) Free Radic. Res. Commun, 9, pp. 101-112
  • Ewing, J.E., Maines, M.D., "Glutathione depletion induces heme oxygenase-1 (HSP32) mRNA and protein in rat brain" (1993) J. Neurochem, 60, pp. 1512-1519
  • Llesuy, S.F., Tomaro, M.L., "Heme oxygenase and oxidative stress. Evidence of involvement of bilirubin as physiological protector against oxidative damage" (1994) Biochim. Biophys. Acta, pp. 9-14. , 1223
  • Yamaguchi, T., Terakado, M., Horio, F., Aoki, K., Tanaka, M., Nakajima, H., "Role of bilirubin as an antioxidant in an ischemia-reperfusion of rat liver and induction of heme oxygenase" (1996) Biochem. Biophys. Res. Commun, 223, pp. 129-135
  • Tomaro, M.L., Frydman, J., Frydman, R.B., "Heme oxygenase induction by CoCl 2, Co-protoporphyrin IX, phenylhydrazine, and diamide: Evidence for oxidative stress involvement" (1991) Arch. Biochem. Biophys, 226, pp. 610-617
  • Sancovich, H.A., Ferramola, A.M., del Batlle, C.A.M., Grinstein, M., "Preparation of porphobilinogen" (1970) Methods in Enzymology, 17, pp. 220-222. , Tabor, H. and Tabot, C.W., eds, (Academic Press, New York)
  • Marver, H.S., Tschudy, D.P., Perlroth, M.G., Collins, A., "δ-aminolevulinic acid synthetase I. Studies in liver homogenates" (1966) J. Biol. Chem, 241, pp. 2803-2809
  • Maines, M.D., Trakshel, G.M., Kutty, R.K., "Characterization of two constitutive forms of rat liver microsomal heme oxygenase" (1986) J. Biol. Chem, 261, pp. 411-419
  • Chance, B., Maehly, A., "Assay of catalases and peroxidases" (1955) Methods in Enzymology, 2, pp. 764-768. , Colowick, S.P. and Kaplan, N.O., eds, (Academic Press, New York)
  • Flohé, L., Gunzler, W.A., "Assays of glutathione peroxidase" (1984) Methods in Enzymology, 105, pp. 114-121. , Packer, L., ed, (Academic Press, New York)
  • Misra, H.P., Fridovich, I., "The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase" (1972) J. Biol. Chem, 247, pp. 3170-3175
  • del Batlle, C.A.M., Ferramola, A.M., Grinstein, M., "Purification and general properties of δ-aminolaevulate dehydratase from cow liver" (1967) Biochem. J, 104, pp. 244-248
  • del Batlle, C.A.M., Wider de Xifra, E.A., Stella, A.M., "A simple method for measuring erythrocyte porphobilinogenase and its use in the diagnosis of acute intermittent porphyria" (1978) Int. J. Biochem, 9, pp. 871-873
  • Rimington, C., "Spectral absorption coefficients of some porphyrins in the Soret band region" (1960) Biochem. J, 193, pp. 620-623
  • Buege, J.A., Aust, S.D., "Microsomal lipid Peroxidation" (1978) Methods in Enzymology, 52, pp. 302-310. , Packer, L., ed, (Academic Press, New York)
  • Anderson, M.E., "Determination of glutathione and glutathione disulfide in biological samples" (1985) Methods in Enzymology, 113, pp. 548-555. , Meister, A., ed, (Academic Press, San Diego)
  • Foresti, R., Clark, J.E., Green, C.J., Motterlini, R., "Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells" (1997) J. Biol. Chem, 272, pp. 18411-18417
  • Lowry, H.O., Rosebrough, N.J., Farr, A.L., Randall, R.J., "Protein measurement with the Folin reagent" (1951) J. Biol. Chem, 193, pp. 265-275
  • Noriega, G.O., Ossola, J.O., Tomaro, M.L., del Batlle, C.A.M., "Effect of acetaminophen on heme metabolism in rat liver" (2000) Int. J. Biochem. Cell Biol, 32, pp. 983-991
  • Piotrowski, W.J., Pietras, T., Zurmanowska, Z., Nowak, D., Marczac, J., Marks-Konczalik, J., Mazerant, P., "Effect of Paraquat intoxication and ambroxol treatment on hydrogen peroxide production and lipid peroxidation in selected organs of rat" (1996) J. Appl. Toxicol, 16, pp. 501-507
  • Sies, H., "On the biochemistry of the thiol group. The role of glutathione" (1989) Naturwissenschaften, 76, pp. 57-64
  • Meister, A., "Glutathione metabolism and its selective modification" (1988) J. Biol. Chem, 263, pp. 17205-17208
  • Arrigo, A.P., "Gene expression and the thiol redox state" (1999) Free Radic. Biol. Med, 27, pp. 936-944
  • Meister, A., Anderson, M.E., "Glutathione" (1983) Annu. Rev. Biochem, 52, pp. 711-760. , Snell, E.E., Boyer, P.D., Meister, A. and Richardson, C.C., eds
  • Pigeolet, E., Corbisier, P., Houbion, A., Lambert, D., Michiels, C., Raes, M., Zachary, M., Remacle, J., "Gluathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides and oxygen derivered free radicals" (1990) Mech. Ageing Dev, 51, pp. 283-297
  • Nakanishi, Y., Yasumoto, K., "Induction after administering paraquat of heme-oxygenase-1 and heat schock protein 70 in the liver of senescenece-accelerated mice" (1997) Biosci. Biotechnol. Biochem, 61 (8), pp. 1302-1306
  • del C. Batlle, A.M., "Porphyrins, porphyrias, canceer and photodynamic therapy - A model for carcinogenesis" (1993) J. Photochem. Photobiol, 20, pp. 5-22
  • Lichtman, H.C., Feldman, F., "In vitro pyrrole and porphyrin synthesis, in lead poisoning" (1963) J. Clin. Invest, 42, pp. 830-839
  • Warren, M., Jordan, P., "Investigation into the nature of substrate binding to the dipyrromethane cofactor of E. Coli porphobilinogen deaminase" (1988) Am. Chem. Soc, 27 (25), pp. 9020-9030
  • Chen, H., Pellett, L.J., Andersen, H.J., Tappel, A.L., "Protection by vitamin E, selenium, and β-carotene against oxidative damage in rat liver slices and homogenate" (1993) Free Radic. Biol. Med, 14, pp. 473-482

Citas:

---------- APA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M. & Batlle, C. (2002) . Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radical Research, 36(6), 633-639.
http://dx.doi.org/10.1080/10715760290029065
---------- CHICAGO ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. "Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase" . Free Radical Research 36, no. 6 (2002) : 633-639.
http://dx.doi.org/10.1080/10715760290029065
---------- MLA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. "Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase" . Free Radical Research, vol. 36, no. 6, 2002, pp. 633-639.
http://dx.doi.org/10.1080/10715760290029065
---------- VANCOUVER ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radic. Res. 2002;36(6):633-639.
http://dx.doi.org/10.1080/10715760290029065