Abstract:
The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase was decreased 3 h after paraquat injection. Heme oxygenase-1 induction started 9 h after treatment, peaking at 15 h. δ-aminolevulinic acid synthase induction occurred once heme oxygenase had been enhanced, reaching its maximum (1.5-fold of control) at 16 h. δ-aminolevulinic acid dehydratase activity was 40% inhibited at 3 h showing a profile similar to that of GSH, while porphobilinogenase activity was not modified along the whole period of the assay. Administration of α-tocopherol (35 mmol/kg body weight) 2 h before paraquat treatment entirely prevented the increase in thiobarbituric acid reactive substances (TBARS) content, the decrease in GSH levels as well as heme oxygenase-1 and δ-aminolevulinic acid synthase induction. This study shows that oxidative stress produced by paraquat leads to an increase in δ-aminolevulinic acid synthase and heme oxygenase-1 activities, indicating that the herbicide affects both heme biosynthesis and degradation.
Registro:
Documento: |
Artículo
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Título: | Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
Autor: | Noriega, G.O.; Gonzales, S.; Tomaro, M.L.; Del, A.M.; Batlle, C. |
Filiación: | Centro de Investigaciones Sobre Porfirinas y Porfirias (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Química Biológica, Universidad de Buenos, Junín 956, Buenos Aires -1113, Argentina
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Palabras clave: | δ-aminolevulinic acid synthase; Antioxidant enzymes; Heme metabolism; Heme oxygenase-1; Oxidative stress; Paraquat; 5 aminolevulinate synthase; alpha tocopherol; antioxidant; catalase; glutathione; glutathione peroxidase; heme; heme oxygenase 1; herbicide; lipid; liver enzyme; paraquat; porphobilinogen synthase; superoxide dismutase; thiobarbituric acid reactive substance; article; biosynthesis; body weight; controlled study; degradation; enzyme activity; enzyme assay; enzyme inhibition; in vivo study; lipid peroxidation; liver metabolism; nonhuman; oxidative stress; porphyrin metabolism; rat; 5-Aminolevulinate Synthetase; alpha-Tocopherol; Animals; Antioxidants; Enzymes; Female; Glutathione; Heme; Heme Oxygenase (Decyclizing); Heme Oxygenase-1; Herbicides; Liver; Oxidative Stress; Paraquat; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances |
Año: | 2002
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Volumen: | 36
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Número: | 6
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Página de inicio: | 633
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Página de fin: | 639
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DOI: |
http://dx.doi.org/10.1080/10715760290029065 |
Título revista: | Free Radical Research
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Título revista abreviado: | Free Radic. Res.
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ISSN: | 10715762
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CODEN: | FRARE
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CAS: | 5-Aminolevulinate Synthetase, EC 2.3.1.37; alpha-Tocopherol, 59-02-9; Antioxidants; Enzymes; Glutathione, 70-18-8; Heme Oxygenase (Decyclizing), EC 1.14.99.3; Heme Oxygenase-1, EC 1.14.99.3; Heme, 14875-96-8; Herbicides; Paraquat, 4685-14-7; Thiobarbituric Acid Reactive Substances
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10715762_v36_n6_p633_Noriega |
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Citas:
---------- APA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M. & Batlle, C.
(2002)
. Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radical Research, 36(6), 633-639.
http://dx.doi.org/10.1080/10715760290029065---------- CHICAGO ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C.
"Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase"
. Free Radical Research 36, no. 6
(2002) : 633-639.
http://dx.doi.org/10.1080/10715760290029065---------- MLA ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C.
"Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase"
. Free Radical Research, vol. 36, no. 6, 2002, pp. 633-639.
http://dx.doi.org/10.1080/10715760290029065---------- VANCOUVER ----------
Noriega, G.O., Gonzales, S., Tomaro, M.L., Del, A.M., Batlle, C. Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Free Radic. Res. 2002;36(6):633-639.
http://dx.doi.org/10.1080/10715760290029065