Artículo

Díaz Añel, A.M.; Rossi, M.S.; Espinosa, J.M.; Güida, C.; Freitas, F.A.; Kornblihtt, A.R.; Zingales, B.; Flawiá, M.M.; Torres, H.N. "mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi" (2000) Journal of Eukaryotic Microbiology. 47(6):555-560
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Abstract:

Differential display of mRNAs from Trypanosoma cruzi epimastigote and metacyclic trypomastigote stages showed several mRNA species differing in their expression level. The cDNA corresponding to one of these mRNAs was used as a probe in Northern blots and identified a RNA product of 2.6 kb with an expression level eight or more times higher in trypomastigotes than in epimastigotes. This probe was also used to screen a genomic library of T. cruzi CL Brener clone prepared in lambda FIX. A clone of about 15 kb was selected that, after partial sequencing, revealed an open reading frame of 688 amino acids encoding a deduced protein with similarity to RNA helicases of the DEAD-box gene family. The presence of the eight conserved motifs characteristic of the DEAD protein family was observed in the T. cruzi sequence, indicating that it corresponds to a putative RNA helicase gene, which we named HelTc. Southern blot analysis indicated that HelTc is a single-copy gene. Pulsed-field gel electrophoresis separation of chromosomes of several isolates of T. cruzi showed that this gene was localized in one or two chromosomal bands.

Registro:

Documento: Artículo
Título:mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi
Autor:Díaz Añel, A.M.; Rossi, M.S.; Espinosa, J.M.; Güida, C.; Freitas, F.A.; Kornblihtt, A.R.; Zingales, B.; Flawiá, M.M.; Torres, H.N.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Laboratorio de Fisiología y Biologia Molecular, Departamento de Ciencias Biológicas, Universidad de Buenos Aires, Argentina
Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05599-970 São Paulo, SP, Brazil
Center for Molecular Genetics, University of California, San Diego, San Diego, CA 92093, United States
Palabras clave:Adenosine triphosphatase; Complementary DNA; Cyclic adenosine monophosphate; Liver infusion tryptose; Messenger RNA; Metacyclogenesis; Northern blot; Proteins with Asp-Glu-Ala-Asp motif; Southern blot; Trypanosoma brucei; blot technique; chromosomal localization; clone; complementary DNA; DEAD box gene; differential display; epimastigote; messenger RNA; open reading frame; RNA helicase; sequence homology; trypomastigote; Eukaryota; Protozoa; Trypanosoma; Trypanosoma brucei; Trypanosoma cruzi; Trypanosoma cruzi
Año:2000
Volumen:47
Número:6
Página de inicio:555
Página de fin:560
DOI: http://dx.doi.org/10.1111/j.1550-7408.2000.tb00089.x
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. Eukaryotic Microbiol.
ISSN:10665234
CODEN:JEMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v47_n6_p555_DiazAnel

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Citas:

---------- APA ----------
Díaz Añel, A.M., Rossi, M.S., Espinosa, J.M., Güida, C., Freitas, F.A., Kornblihtt, A.R., Zingales, B.,..., Torres, H.N. (2000) . mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi. Journal of Eukaryotic Microbiology, 47(6), 555-560.
http://dx.doi.org/10.1111/j.1550-7408.2000.tb00089.x
---------- CHICAGO ----------
Díaz Añel, A.M., Rossi, M.S., Espinosa, J.M., Güida, C., Freitas, F.A., Kornblihtt, A.R., et al. "mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi" . Journal of Eukaryotic Microbiology 47, no. 6 (2000) : 555-560.
http://dx.doi.org/10.1111/j.1550-7408.2000.tb00089.x
---------- MLA ----------
Díaz Añel, A.M., Rossi, M.S., Espinosa, J.M., Güida, C., Freitas, F.A., Kornblihtt, A.R., et al. "mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi" . Journal of Eukaryotic Microbiology, vol. 47, no. 6, 2000, pp. 555-560.
http://dx.doi.org/10.1111/j.1550-7408.2000.tb00089.x
---------- VANCOUVER ----------
Díaz Añel, A.M., Rossi, M.S., Espinosa, J.M., Güida, C., Freitas, F.A., Kornblihtt, A.R., et al. mRNA encoding a putative RNA helicase of the DEAD-box gene family is up-regulated in trypomastigotes of Trypanosoma cruzi. J. Eukaryotic Microbiol. 2000;47(6):555-560.
http://dx.doi.org/10.1111/j.1550-7408.2000.tb00089.x