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Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Flawiá, M.M.; Torres, H.N. "L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi" (1999) Journal of Eukaryotic Microbiology. 46(6):566-570
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Abstract:

A very specific L-arginine transporter showing high affinity has been characterized in Trypanosoma cruzi epimastigotes. Uptake was found to be dependent on L-arginine concentration and it was saturable. Values for maximum velocity and Km ranged between 48.1-57.5 pmol·min-1 per 3 x 107 cells and between 4.2-5.5 μM, respectively The calculated activation energy and Q10 were 31.1 KJ·mol-1, and 1.7, respectively. Uptake velocity significantly increased when cells were preincubated in the absence of L-arginine. Cells retained the labeled amino acid independently of the presence or absence of exogenous L-arginine. The specificity of L-arginine uptake was demonstrated by competition assays in the presence of 80-fold molar excess of natural amino acids and several L-arginine derivatives. The highest levels of inhibition were caused by L-homoarginine. D-arginine, L-canavanine, L-ornithine, and L-citrulline, L-arginine uptake by T. cruzi epimastigotes was not affected by the presence of potassium or sodium ions in the incubation mixture or by pH changes in the range between 5.5-8.5. The major product of L-arginine uptake was characterized as phosphoarginine. Moreover, arginine kinase activity was detected in soluble extracts from T. cruzi epimastigotes.

Registro:

Documento: Artículo
Título:L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi
Autor:Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Flawiá, M.M.; Torres, H.N.
Filiación:Inst. Invest. Ing. Genetica y B., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Amino acid transport; Arginine kinase; Canavanine; Epimastigotes; Homoarginine; Phosphagen; amino acid synthesis; amino acid transport; arginine; canavanine; citrulline; energy metabolism; homoarginine; microbe metabolism; ornithine; phosphoarginine; Trypanosoma; Trypanosoma cruzi; Trypanosoma cruzi
Año:1999
Volumen:46
Número:6
Página de inicio:566
Página de fin:570
DOI: http://dx.doi.org/10.1111/j.1550-7408.1999.tb05132.x
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. Eukaryotic Microbiol.
ISSN:10665234
CODEN:JEMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v46_n6_p566_Pereira

Referencias:

  • Ariyanayagam, M.R., Fairlamb, A.H., Diamine auxotrophy may be a universal feature of Trypanosoma cruzi epimastigotes (1997) Mol. Biochem. Parasitol., 84, pp. 111-121
  • Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding (1976) Analyt. Biochem., 72, pp. 248-254
  • Camargo, E.P., Coelho, J.A., Moraes, G., Figueiredo, E.N., Trypanosoma spp., Leishmania spp. and Leptomonas spp.: Enzymes of ornithine-arginine metabolism (1978) Exp. Parasitol., 46, pp. 141-144
  • Camargo, E.P., Silva, S., Roitman, I., De Souza, W., Jankevicius, J.V., Dollet, M., Enzymes of the ornithine-arginine metabolism in Trypanosomatids of genus Phytomonas (1987) J. Protozool., 34, pp. 439-441
  • Cohn, C.S., Gottlieb, M., The acquisition of purines by Trypanosomatids (1997) Parasitology Today, 13, pp. 231-235
  • Dixon, M., Webb, E.C., (1964) Enzymes, 2d Ed., pp. 67-70. , Longmans, Green & Co., London
  • Fairlamb, A.H., Blackburn, P., Ulrich, P., Chait, B.T., Cerami, A., Trypanothione: A novel bis(glutathionyl)-spermidine cofactor for glutathione reductase in trypanosomatids (1985) Science, 227, pp. 1485-1487
  • France, R.M., Cellers, D.S., Grossman, S.H., Purification, characterization, and hydrodynamic properties of arginine kinase from Gulf Shrimp (Penaeus aztecus) (1997) Arch. Biochem. Biophys., 345, pp. 73-78
  • Goldberg, S.S., Pereira, A.A., Chiari, E., Mares-Guia, M., Gazzinelli, G., Comparative kinetics of arginine and lysine transport by epimastigotes and trypomastigotes from two strains of Trypanosoma cruzi (1976) J. Protozool., 23, pp. 179-186
  • Hampton, J.R., Arginine transport in the culture form of Trypanosoma cruzi (1971) J. Protozool., 18, pp. 701-703
  • Hird, F.J.R., The importance of arginine in evolution (1986) Comp. Biochem. Physiol., 858, pp. 285-288
  • Hird, F.J.R., Cianciosi, S.C., McLean, R.M., Niekrash, R.E., On the possible significance of the transamidination reaction in evolution (1983) Camp. Biochem. Physiol., 76 B, pp. 489-495
  • Huennekens, F.M., Whiteley, H.R., Phosphoric acid anhydrides and other energy-rich compounds (1960) Comparative Biochemistry, 1, pp. 107-180. , Florkin, M. & Mason, H.S. (ed.), Academic Press, New York
  • Hunter, K.J., Le Quesne, S.A., Fairlamb, A.H., Identification and biosynthesis of N1, N9-bis(glutathionyl)aminopropyl-cadaverine (homotrypanothione) in Trypanosoma cruzi (1994) Eur. J. Biochem., 226, pp. 1019-1027
  • Kandpal, M., Fouce, R.B., Pal, A., Guru, P.Y., Tekwani, B.L., Kinetics and molecular characteristics of arginine transport by Leishmania donovani promastigotes (1995) Mol. Biochem. Parasitol., 71, pp. 193-201
  • Le Quesne, S.A., Fairlamb, A.L., Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes (1996) Biochem. J., 316, pp. 481-486
  • Le Quesne, S.A., Fairlamb, A.H., Measurement of polyamine transport (1997) Methods in Molecular Biology, 79, pp. 149-156. , Morgan, D. (ed.), Humana Press Inc., Totowa, N. J
  • Low, B.C., Grigor, M.R., Angiotensin II stimulates system y+ and cationic amino acid transporter gene expression in cultured vascular smooth muscle cells (1995) J. Biol. Chem., 270, pp. 27577-27583
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • McGivan, J.D., Pastor-Anglada, M., Regulatory and molecular aspects of mammalian amino acid transport (1994) Biochem. J., 299, pp. 321-334
  • Morrison, J.F., Arginine kinase and other guanidino kinases (1973) The Enzymes, 8, pp. 457-486. , Boyer, P.D. (ed.), Academic Press, New York
  • Palmer, R.M.J., Ferrige, A.G., Moncada, S., Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor (1987) Nature, 327, pp. 524-526
  • Paveto, C., Pereira, C., Espinosa, J., Montagna, A.E., Farber, M., Esteva, M., Flawiá, M.M., Torres, H.N., The nitric oxide pathway in Trypanosoma cruzi (1995) J. Biol. Chem., 270, pp. 16576-16579
  • Pereira, C., Paveto, C., Espinosa, J., Alonso, G., Flawiá, M.M., Torres, H.N., Control of Trypanosoma cruzi epimastigote motility through the nitric oxide pathway (1997) J. Eukaryot. Microbiol., 44, pp. 155-156
  • Richards, D.E., Rega, A.F., Garrahan, P.J., Two classes of sites for ATP in the Ca2+-ATPase from human red cell membranes (1978) Biochim. Biophys. Acta., 511, pp. 194-201
  • Tabor, C.W., Tabor, H., Polyamines (1984) Ann. Rev. Biochem., 53, pp. 749-790
  • White, M.F., The transport of cationic amino acids across the plasma membrane of mammalian cells (1985) Biochim. Biophys. Acta, 822, pp. 355-374
  • White, M.F., Christensen, H.N., Cationic amino acid transport into culture animal cells. II Transport system barely perceptible in ordinary hepatocytes, but active in hepatoma cell lines (1982) J. Biol. Chem., 257, pp. 4450-4457
  • Yoshida, N., Camargo, E.P., Urotelism and ammonotelism in trypanosomatids (1978) J. Bacteriol., 136, pp. 1184-1185
  • Yu, L., Fengaro, P.E., Niederberg, M., Burke, T.J., Schrier, R.W., Nitric oxide: A mediator in rat tubular hypoxia/reoxygenation injury (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 1691-1695

Citas:

---------- APA ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Flawiá, M.M. & Torres, H.N. (1999) . L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi. Journal of Eukaryotic Microbiology, 46(6), 566-570.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb05132.x
---------- CHICAGO ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Flawiá, M.M., Torres, H.N. "L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi" . Journal of Eukaryotic Microbiology 46, no. 6 (1999) : 566-570.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb05132.x
---------- MLA ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Flawiá, M.M., Torres, H.N. "L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi" . Journal of Eukaryotic Microbiology, vol. 46, no. 6, 1999, pp. 566-570.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb05132.x
---------- VANCOUVER ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Flawiá, M.M., Torres, H.N. L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi. J. Eukaryotic Microbiol. 1999;46(6):566-570.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb05132.x