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Espinosa, J.; Martinetto, H.; Portal, D.; D'Angelo, M.; Torres, H.N.; Flawiá, M.M. "Factors from Trypanosoma cruzi interacting with AP-1 sequences" (1999) Journal of Eukaryotic Microbiology. 46(5):516-521
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Abstract:

Interaction between factors from Trypanosoma cruzi extracts and AP- 1 sequences was studied by electrophoretic mobility shift assays. Using a double-stranded probe carrying the AP-1 sequence from the SV40 promoter, three specific complexes designated A, B, and C were detected. Complexes A and C were formed when using single-stranded probes. The relative amount of complex B, specific for double-stranded DNA, increased as a function of probe length. Complexes were stabilized by cross-linking with UVC irradiation and resolved on denaturing SDS-PAGE. Complex A generated bands of 60- and 39 kDa; complex B produced two bands of 46- and 43 kDa; and complex C generated one band of 43 kDa. The AP-I binding activity was much higher in purified nuclear preparations than in soluble fractions, and was detected in crude extracts from the three forms of the parasite. The binding signal, however, was much stronger in amastigote and trypomastigote than in the epimastigote forms. Specific binding was increased by oxidative stress. Antibodies raised against peptides corresponding to conserved domains of mammalian c-Jun and c-Fos detected bands of 40- and 60 kDa, respectively, in a nuclear epimastigote preparation.

Registro:

Documento: Artículo
Título:Factors from Trypanosoma cruzi interacting with AP-1 sequences
Autor:Espinosa, J.; Martinetto, H.; Portal, D.; D'Angelo, M.; Torres, H.N.; Flawiá, M.M.
Filiación:Inst. Invest. Ing. Genet. Biol. M., Consejo Nac. de Invest. Cie. y Tec., Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:c-Fos; c-Jun; DNA binding proteins; Gel shift analysis; Oxidative stress; Transcription; cross linking; electrophoretic mobility; molecular genetics; probe; SDS polyacrylamide gel electrophoresis; sequence analysis; Mammalia; Protozoa; Trypanosoma; Trypanosoma cruzi; Trypanosoma cruzi
Año:1999
Volumen:46
Número:5
Página de inicio:516
Página de fin:521
DOI: http://dx.doi.org/10.1111/j.1550-7408.1999.tb06069.x
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. Eukaryotic Microbiol.
ISSN:10665234
CODEN:JEMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v46_n5_p516_Espinosa

Referencias:

  • Andrews, N.W., Colli, W., Adhesion and interiorization of Trypanosoma cruzi in mammalian cells (1982) J. Protozool., 29, pp. 264-269
  • Bell, S.D., Barry, J.D., Trypanosome nuclear factors which bind to internal promoter elements of tRNA genes (1995) Nucl. Acids Res., 16, pp. 3103-3110
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Curran, T., Franza, B.R., Fos and Jun: The AP-1 connection (1988) Cell, 55, pp. 395-397
  • Datta, R., Hallahan, D.E., Kharbanda, S.M., Rubin, E., Sherman, M.L., Huberman, E., Weichselbaum, R.R., Kule, D.W., Involvement of reactive oxygen intermediates in the induction of c-jun gene transcription by ionizing radiation (1992) Biochemistry, 31, pp. 8300-8306
  • Fraidenraich, D., Peña, C., Isola, E.L., Lammel, E.M., Coso, O., Añel, A.D., Pongor, S., Flawiá, M.M., Stimulation of Trypanosoma cruzi adenilyl cyclase by an αD-globin fragment from Triatoma hindgut. Effect on differentiation of epimastigote to trypomastigote form (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 10140-10144
  • Garcia, E.S., Gonzalez, M.S., De Azambuja, P., Baralle, F.E., Fraidenraich, D., Torres, H.N., Flawiá, M.M., Induction of Trypanosoma cruzi metacyclogenesis in gut of the hematophagous insect vector, Rhodnius prolixus, by hemoglobin and peptides carrying αD-globin sequences (1995) Exper. Parasitol., 81, pp. 255-261
  • Gómez, M.L., Erijman, L., Arauzo, S., Torres, H.N., Téllez-Iñón, M.T., Protein kinase C in Trypanosoma cruzi epimastigote forms. Partial purification and characterization (1989) Mol. Biochem. Parasitol., 36, pp. 101-108
  • Gonzalez-Perdomo, M., Romero, P., Goldenberg, S., Cyclic AMP and adenilate cyclase stimulators stimulate Trypanosoma cruzi differentiation (1988) Exp. Parasitol., 66, pp. 205-212
  • Isola, E.L., Lammel, E., Gonzalez-Cappa, S.M., Trypanosoma cruzi differentiation after interaction of epimastigotes and Triatoma infestans intestinal homogenate (1986) Exp. Parasitol., 62, pp. 329-335
  • Karin, M., Zheng-gang, L., Zandi, E., AP-1 function and regulation (1997) Curr. Op. Cell Biol., 9, pp. 240-246
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Pays, E., Vanhamme, L., Developmental regulation of gene expression in African trypanosomes (1996) Molecular Biology of Parasitic Protozoa, pp. 88-114. , Smith, D. F. and Parsons, M. (ed.), IRL Press, Oxford
  • Rauscher III, F.J., Cohen, D.R., Curran, T., Bos, T.J., Vogt, P.K., Bohman, D., Tijan, R., Franza, R.R., Fos-associated protein p39 is the product of the jun proto-oncogene (1988) Science, 240, pp. 1010-1016
  • Swindle, J., Ajioka, J., Eisen, H., Sanwal, B., Jacquemont, C., Browder, Z., Buck, G., The genomic organization and transcription of the ubiquitin genes of Trypanosoma cruzi (1988) EMBO J., 7, pp. 1121-1127
  • Teixeira, S.M., Kirchhoff, L.V., Donelson, J.E., Post-transcriptional elements regulating expression of mRNAs from the amastin/ tuzin gene cluster of Trypanosoma cruzi (1995) J. Biol. Chem., 270, pp. 22586-22594
  • Ulloa, R.M., Mesri, E., Esteva, M., Torres, H.N., Téllez-Iñón, M.T., Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi (1988) Biochem. J., 255, pp. 319-326
  • Vanhamme, L., Pays, A., Tebabi, P., Alexandre, S., Pays, E., Specific binding of proteins to the non-coding strand of a crucial element of the variant surface glycoprotein, procyclin and ribosomal promoters of Trypanosoma brucei (1995) Mol. Cell. Biol., 15, pp. 5598-5606
  • Vogt, P.K., Bos, T.J., Doolittle, R.F., Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 3316-3319
  • Webb, J.R., McMaster, W.R., Molecular cloning and expression of a Leishmania major gene encoding a single-stranded DNA-binding protein containing nine "CCHC" zinc finger motifs (1993) J. Biol. Chem., 268, pp. 13994-14002
  • Yamamoto, K.K., Gonzalez, G.A., Biggs III, W.H., Montminy, M.R., Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB (1988) Nature, 334, pp. 494-498

Citas:

---------- APA ----------
Espinosa, J., Martinetto, H., Portal, D., D'Angelo, M., Torres, H.N. & Flawiá, M.M. (1999) . Factors from Trypanosoma cruzi interacting with AP-1 sequences. Journal of Eukaryotic Microbiology, 46(5), 516-521.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb06069.x
---------- CHICAGO ----------
Espinosa, J., Martinetto, H., Portal, D., D'Angelo, M., Torres, H.N., Flawiá, M.M. "Factors from Trypanosoma cruzi interacting with AP-1 sequences" . Journal of Eukaryotic Microbiology 46, no. 5 (1999) : 516-521.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb06069.x
---------- MLA ----------
Espinosa, J., Martinetto, H., Portal, D., D'Angelo, M., Torres, H.N., Flawiá, M.M. "Factors from Trypanosoma cruzi interacting with AP-1 sequences" . Journal of Eukaryotic Microbiology, vol. 46, no. 5, 1999, pp. 516-521.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb06069.x
---------- VANCOUVER ----------
Espinosa, J., Martinetto, H., Portal, D., D'Angelo, M., Torres, H.N., Flawiá, M.M. Factors from Trypanosoma cruzi interacting with AP-1 sequences. J. Eukaryotic Microbiol. 1999;46(5):516-521.
http://dx.doi.org/10.1111/j.1550-7408.1999.tb06069.x