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Abstract:

The presence of Ca2+/calmodulin (Ca2+/CaM)-dependent protein kinase (TcCaM K) and some stage-specific substrates that appeared during morphogenesis of the parasite Trypanosoma cruzi were identified. Western blot analysis using a polyclonal antibody against rat brain CaM K type II recognized the same subunit composition (52, 59/62 kDa) observed for the mammalian enzyme, as well as the previously characterized TcCaM K found in epimastigote forms. Differential protein phosphorylation profiles were observed after enzyme activation in the stages of T. cruzi. Co-immunoprecipitation of stage-specific substrates with the TcCaM K suggested that the enzyme might be involved in the phosphorylation of a different set of proteins through the life cycle. Three phosphoproteins, pp105 and pp87 from epimastigotes and pp23 from trypomastigotes were identified as potential substrates for TcCaM K. The characterization of these endogenous stage markers might be a useful tool to understand the developmental cycles of these pathogenic protozoa.

Registro:

Documento: Artículo
Título:Stage-specific substrate phosphorylation by a Ca2+/calmodulin-dependent protein kinase in Trypanosoma cruzi
Autor:Ogueta, S.B.; Macintosh, G.C.; Téllez-Iñon, M.T.
Filiación:Inst. Invest. Ing. Genet. Biol. M., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, United States
Michigan State University, Department of Energy, Michigan State University, East Lansing, MI 48824, United States
Palabras clave:Amastigotes; Differentiation; Epimastigotes; Trypanosomatids; Trypomastigotes; calcium; calmodulin; developmental cycle; enzyme activation; identification; morphogenesis; phosphorylation; polyclonal antibody; protein kinase; Western blotting; Trypanosoma cruzi
Año:1998
Volumen:45
Número:4
Página de inicio:392
Página de fin:396
DOI: http://dx.doi.org/10.1111/j.1550-7408.1998.tb05089.x
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. Eukaryotic Microbiol.
ISSN:10665234
CODEN:JEMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v45_n4_p392_Ogueta

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Citas:

---------- APA ----------
Ogueta, S.B., Macintosh, G.C. & Téllez-Iñon, M.T. (1998) . Stage-specific substrate phosphorylation by a Ca2+/calmodulin-dependent protein kinase in Trypanosoma cruzi. Journal of Eukaryotic Microbiology, 45(4), 392-396.
http://dx.doi.org/10.1111/j.1550-7408.1998.tb05089.x
---------- CHICAGO ----------
Ogueta, S.B., Macintosh, G.C., Téllez-Iñon, M.T. "Stage-specific substrate phosphorylation by a Ca2+/calmodulin-dependent protein kinase in Trypanosoma cruzi" . Journal of Eukaryotic Microbiology 45, no. 4 (1998) : 392-396.
http://dx.doi.org/10.1111/j.1550-7408.1998.tb05089.x
---------- MLA ----------
Ogueta, S.B., Macintosh, G.C., Téllez-Iñon, M.T. "Stage-specific substrate phosphorylation by a Ca2+/calmodulin-dependent protein kinase in Trypanosoma cruzi" . Journal of Eukaryotic Microbiology, vol. 45, no. 4, 1998, pp. 392-396.
http://dx.doi.org/10.1111/j.1550-7408.1998.tb05089.x
---------- VANCOUVER ----------
Ogueta, S.B., Macintosh, G.C., Téllez-Iñon, M.T. Stage-specific substrate phosphorylation by a Ca2+/calmodulin-dependent protein kinase in Trypanosoma cruzi. J. Eukaryotic Microbiol. 1998;45(4):392-396.
http://dx.doi.org/10.1111/j.1550-7408.1998.tb05089.x