Artículo

Ochatt, C.M.; Mayorga, L.S.; Isola, E.L.D.; Wilkowsky, S.; Torres, H.N.; Téllez-Iñón, M.T. "Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol" (1997) Journal of Eukaryotic Microbiology. 44(5):497-502
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Trypanosoma cruzi trypomastigotes survive inside macrophages by promoting fusion between the parasitophorous vacuole and mature host lysosomes upon internalization. Since trypomastigotes can evade the lyric pathway, the earliest steps of endocytosis, such as early endosome fusion, may be affected. To test this hypothesis, we used an in vitro early endosome fusion assay. Our results show that trypomastigote-infected macrophage cytosols cannot promote fusion between early endosomes, compared to mock-infected cytosols (heat-killed trypomastigotes were used in the parasite-macrophage interaction assay). GTPγS addition potentiates the fusogenic activity driven by trypomastigote-infected macrophage cytosol-mediated assays, unlike the biphasic fusogenic effect obtained with GTPγS treatment of macrophage cytosol controls. Calcium-stimulated early endosome fusogenic processes are not affected in the assays mediated by infected macrophage cytosol. We conclude that GTP-regulated factors, and not calcium-regulated elements, are involved in the inhibition of the early endosome fusogenic process by the trypomastigote-infected macrophage cytosol. This primary impediment to the progress of a normal endocytosis may be a relevant step required for the lysosomal recruitment-fusion of the host lysosomes upon trypomastigote infection and further survival of the parasite within its host.

Registro:

Documento: Artículo
Título:Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
Autor:Ochatt, C.M.; Mayorga, L.S.; Isola, E.L.D.; Wilkowsky, S.; Torres, H.N.; Téllez-Iñón, M.T.
Filiación:Inst. Invest. Ing., Genet. y B., Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Inst. de Histol., y Embriol., Facultad Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina
Departamento de Microbiología, Facultad de Medicina, Universidad Buenos Aires, Buenos Aires, Argentina
Laboratory of Molecular Parasitology, Rockefeller University, 1230 York Ave., New York, NY 10021, United States
Palabras clave:Calcium supply; GTPγS; Trypomastigote-infected macrophage; Animalia; Trypanosoma; Trypanosoma cruzi
Año:1997
Volumen:44
Número:5
Página de inicio:497
Página de fin:502
DOI: http://dx.doi.org/10.1111/j.1550-7408.1997.tb05730.x
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. EUKARYOTIC MICROBIOL.
ISSN:10665234
CODEN:JEMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v44_n5_p497_Ochatt

Referencias:

  • Allen, R.D., Schroeder, L.C., Fok, A.K., Endosomal system of Paramecium: Coated pits to early endosomes (1992) J. Cell. Sci., 101, pp. 449-461
  • Andrews, N.W., What we talk about when we talk about cell invasion: Reflections on a Trypanosome system (1995) Molecular Approaches to Parasitology, pp. 359-369. , Boothroyd, J. C. & Komuniecki, R. (ed.). Wiley-Liss, Inc., New York
  • Araujo-Jorge, T.C., Sampaio, E.P., De Souza, W., Meirelles, M.N., Trypanosoma cruzi: The effect of variations in experimental conditions on the levels of macrophage infections in vitro (1989) Parasitol. Res., 75, pp. 257-263
  • Brickman, M.J., Balber, A.E., Trypanosoma brucei rhodesiense: Membrane glycoproteins localized primarily in endosomes and lysosomes of bloodstream forms (1993) Exp. Parasitol., 76, pp. 329-344
  • Burleigh, B.A., Andrews, N.W., A 120-kDa Alkaline Peptidase from Trypanosoma cruzi is involved in the generation of a novel Ca2+-signaling factor for mammalian cells (1995) J. Biol. Chem., 270, pp. 5172-5180
  • Carvalho, T.V., De Souza, W., Effect of phorbol-12-myristate-13-acetate (PMA) on the fine structure of Trypanosoma cruzi and its interaction with activated and resident macrophages (1987) Parasitol. Res., 74, pp. 11-17
  • Colombo, M.I., Mayorga, L.S., Casey, P.J., Stahl, P.D., Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion (1992) Science, 255, pp. 1695-1697
  • Coso, O.A., Díaz-Añel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Fraidenraich, D., Torres, H.N., Flawia, M.M., Characterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes (1992) Biochem. J., 287, pp. 443-446
  • Diaz, R., Mayorga, L.S., Stahl, P.D., In vitro fusion of endosomes following receptor-mediated endocytosis (1988) J. Biol. Chem., 263, pp. 6093-6100
  • Diment, S., Leech, M.S., Stahl, P.D., Generation of macrophage variants with 5-aza-cytidine: Selection for mannose receptor expression (1987) J. Leukocyte Biol., 42, pp. 485-490
  • González Cappa, S.M., Bijovsky, A.T., Freilij, H., Muller, L., Katzin, A.M., Aislamiento de una cepa de Trypanosoma cruzi a predominio de formas delgadas en la Argentina (1981) Medicina, 41, pp. 119-121
  • Hall, B.F., Furtado, G.C., Joiner, K.A., Characterization of host cell-derived membrane proteins of the vacuole surrounding different intracellular forms of Trypanosoma cruzi in J774 cells. Evidence for phagocyte receptor sorting during the early stages of parasite entry (1991) J. Immunol., 147, pp. 4313-4321
  • Hall, B.F., Webster, P., Ma, A.K., Joiner, K.A., Andrews, N.W., Desialylation of lysosomal membrane glycoproteins by Trypanosoma cruzi: A role for the surface neuraminidase in facilitating parasite entry into the host cell cytoplasm (1992) J. Exp. Med., 176, pp. 313-325
  • Isola, E.L., Lammel, E.M., Gonzalez Cappa, S.M., Trypanosoma cruzi differentiation after interaction of epimastigotes and Triatoma infestans intestinal homogenate (1986) Exp. Parasitol., 62, pp. 329-335
  • Isola, E.L., Lammel, E.M., Redruello, M., Trypanosoma cruzi: Conditions required to improve metacyclic differentiation in axenic culture (1989) Revista Argentina Microbiología, 21, pp. 9-14
  • Joiner, K.A., Da Silva, W.D., Rimoldi, M.T., Hammer, C.H., Sher, A., Kipnis, T.L., Biochemical characterization of a factor produced by trypomastigotes of Trypanosoma cruzi that accelerates the decay of complement C3 convertases (1988) J. Biol. Chem., 263, pp. 11327-11335
  • Keller, R.K., Touster, O., Physical and chemical properties of beta-glucuronidase from the preputial gland of the female rat (1975) J. Biol. Chem., 250, pp. 4765-4769
  • Lenhard, J.M., Mayorga, L.S., Stahl, P.D., Characterization of endosome-endosome fusion in a cell-free system using Dictyostelium discoideum (1992) J. Biol. Chem., 267, pp. 1896-1903
  • Ley, V., Robbins, E.S., Nussenzweig, V., Andrews, N.W., The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments (1990) J. Exp. Med., 171, pp. 401-413
  • Lowry, O.H., Roseborough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Mayorga, L.S., Beron, W., Sarrouf, M.N., Colombo, M.I., Creutz, C., Stahl, P.D., Calcium-dependent fusion among endosomes (1994) J. Biol. Chem., 269, pp. 30927-30934
  • Mayorga, L.S., Diaz, R., Stahl, P.D., Reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis (1989) Methods Cell Biol., 31, pp. 179-196
  • Mayorga, L.S., Diaz, R., Stahl, P.D., Regulatory role for GTP-binding proteins in endocytosis (1989) Science, 244, pp. 1475-1477
  • Mayorga, L.S., Diaz, R., Stahl, P.D., Plasma membrane-derived vesicles containing receptor-ligand complexes are fusogenic with early endosomes in a cell-free system (1988) J. Biol. Chem., 263, pp. 17213-17216
  • Meirelles, M.L., Araujo-Jorge, T.C., De Souza, W., Moreira, A., Barbosa, H.S., Trypanosoma cruzi: Phagolysosomal fusion after invasion into non professional phagocytic cells (1987) Cell Struct. and Function, 12, pp. 387-393
  • Meirelles, M.N., Araujo-Jorge, T.C., Miranda, C.F., De Souza, W., Barbosa, H.S., Interactions of Trypanosoma cruzi with heart muscle cells: Ultrastructural and cytochemical analysis of endocytic vacuole formation and effect upon myogenesis in vitro (1986) Eur. J. Cell Biol., 41, pp. 198-206
  • Mortatti, R.C., Munk, M.E., Separation of bloodstream trypomastigotes of Trypanosoma cruzi by density gradient centrifugation (1985) J. Parasitol., 71, pp. 520-521
  • Nogueira, N., Cohn, Z., Trypanosoma cruzi: Mechanism of entry and intracellular fate in mammalian cells (1976) J. Exp. Med., 143, pp. 1402-1420
  • Rimoldi, M.T., Sher, A., Hieny, S., Lituchy, A., Hammer, C.H., Joiner, K., Developmentally regulated expression by Trypanosoma cruzi of molecules that accelerate the decay of complement C3 convertases (1988) Proc. Natl. Acad. Sci. USA, 85, pp. 193-197
  • Schenkman, S.A., Robbins, E.S., Nussenzweig, V., Attachment of Trypanosoma cruzi to mammalian cells requires parasite energy, and the invasion can be independent of the target cell cytoskeleton (1991) Infect. and Immunol., 59, pp. 645-654
  • Schenkman, S., Andrews, N.W., Nussenzweig, V., Robbins, E.S., Trypanosoma cruzi invade a mammalian epithelial cell in a polarized manner (1988) Cell, 55, pp. 157-165
  • Soares, M.J., Souto-Padron, T., De Souza, W., Identification of a large pre-lysosomal compartment in the pathogenic protozoon Trypanosoma cruzi (1992) J. Cell. Sci., 102, pp. 157-167
  • Tardieux, I., Nathanson, M.H., Andrews, N.W., Role in host cell invasion of Trypanosoma cruzi-induced cytosolic-free Ca2+ transients (1994) J. Exp. Med., 179, pp. 1017-1022
  • Tardieux, I., Webster, P., Ravesloot, J., Boron, W., Lunn, J.A., Heuser, J.E., Andrews, N.W., Lysosome recruitment and fusion are early events required for trypanosome invasion of mammalian cells (1992) Cell, 71, pp. 1117-1130
  • Thomas, L., Clarke, P.R., Pagano, M., Gruenberg, J., Inhibition of membrane fusion in vitro via cyclin B but not cyclin A (1992) J. Biol. Chem., 267, pp. 6183-6187
  • Tuomikoski, T., Felix, M.A., Doree, M., Gruenberg, J., Inhibition of endocytic vesicle fusion in vitro by the cell-cycle control protein kinase cdc2 (1989) Nature, 342, pp. 942-945
  • Zingales, B., Colli, W., Trypanosoma cruzi: Interaction with host cells (1985) Curr. Topics Microbiol. and Immunol., 117, pp. 129-152

Citas:

---------- APA ----------
Ochatt, C.M., Mayorga, L.S., Isola, E.L.D., Wilkowsky, S., Torres, H.N. & Téllez-Iñón, M.T. (1997) . Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol. Journal of Eukaryotic Microbiology, 44(5), 497-502.
http://dx.doi.org/10.1111/j.1550-7408.1997.tb05730.x
---------- CHICAGO ----------
Ochatt, C.M., Mayorga, L.S., Isola, E.L.D., Wilkowsky, S., Torres, H.N., Téllez-Iñón, M.T. "Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol" . Journal of Eukaryotic Microbiology 44, no. 5 (1997) : 497-502.
http://dx.doi.org/10.1111/j.1550-7408.1997.tb05730.x
---------- MLA ----------
Ochatt, C.M., Mayorga, L.S., Isola, E.L.D., Wilkowsky, S., Torres, H.N., Téllez-Iñón, M.T. "Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol" . Journal of Eukaryotic Microbiology, vol. 44, no. 5, 1997, pp. 497-502.
http://dx.doi.org/10.1111/j.1550-7408.1997.tb05730.x
---------- VANCOUVER ----------
Ochatt, C.M., Mayorga, L.S., Isola, E.L.D., Wilkowsky, S., Torres, H.N., Téllez-Iñón, M.T. Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol. J. EUKARYOTIC MICROBIOL. 1997;44(5):497-502.
http://dx.doi.org/10.1111/j.1550-7408.1997.tb05730.x