Farber, M.D.; Montagna, A.E.; Paveto, C.; Dollet, M.; Sanchex-Moreno, M.; Osuna, A.; Torres, H.N.; Flawia, M.M. "Adenyiyl Cyclase and G‐Proteins in Phytomonas" (1995) Journal of Eukaryotic Microbiology. 42(3):257-260
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ABSTRACT. Phytomonas sp. membranes have an adenylyl cyclase activity which is greater in the presence of Mn2+ than with Mg2+. The Mg2+ and Mn2+ activity ratio varies from one membrane preparation to another, suggesting that the adenylyl cyclase has a variable activation state. A [35S]GTP‐γ‐S‐binding activity with a Kd of 171 nM was detected in Phytomonas membranes. Incubation of these membranes with activated cholera or pertussis toxin and [adenylate 32P]NAD+ led to incorporation of radioactivity into bands of about 40–44 kDa. Crude membranes were electrophoresed on SDS‐polyacrylamide gels and analyzed, by Western blotting, with the 9188 anti‐αs antibody and the AS/7 antibody (anti‐α1, anti‐αi1, anti‐αi2). These procedures resulted in the identification of polypeptides of approximately 40–44 kDa. Phytomonas adenylyl cyclase could be activated by treatment of membrane preparations with cholera toxin, in the presence of NAD+, while similar treatment with pertussis toxin did not affect this enzyme activity. These studies indicate that in Phytomonas, adenylyl cyclase activity is coupled to an unknown receptor entity through Gαs, proteins. Copyright © 1995, Wiley Blackwell. All rights reserved


Documento: Artículo
Título:Adenyiyl Cyclase and G‐Proteins in Phytomonas
Autor:Farber, M.D.; Montagna, A.E.; Paveto, C.; Dollet, M.; Sanchex-Moreno, M.; Osuna, A.; Torres, H.N.; Flawia, M.M.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Départment des Cultures Perennes, Centre de Coopération Internationale en Recherche Agronomique pour le Développment, Montpellier, France
Facultad de Ciencias, Universidad de Granada, Granada, Spain
Palabras clave:ADP‐ribosylation; cholera toxin; pertussis toxin; plant trypanosomatids; adenosine diphosphate; adenylate cyclase; adenylate cyclase toxin; bacterial toxin; Bordetella virulence factor; cholera toxin; divalent cation; guanosine triphosphate; heterotrimeric guanine nucleotide binding protein; magnesium; manganese; nicotinamide adenine dinucleotide; pertussis toxin; animal; article; cell membrane; cytology; drug effect; enzyme activation; enzymology; flagellate; metabolism; molecular weight; protein binding; Adenosine Diphosphate; Adenylate Cyclase; Adenylate Cyclase Toxin; Animals; Bacterial Toxins; Cations, Divalent; Cell Membrane; Cholera Toxin; Enzyme Activation; Guanosine Triphosphate; Heterotrimeric GTP-Binding Proteins; Magnesium; Manganese; Molecular Weight; NAD; Pertussis Toxin; Protein Binding; Trypanosomatina; Virulence Factors, Bordetella
Página de inicio:257
Página de fin:260
Título revista:Journal of Eukaryotic Microbiology
Título revista abreviado:J. Eukaryotic Microbiol.
CAS:Adenosine Diphosphate, 58-64-0; Adenylate Cyclase Toxin; Adenylate Cyclase, EC; Bacterial Toxins; Cations, Divalent; Cholera Toxin, 9012-63-9; Guanosine Triphosphate, 86-01-1; Heterotrimeric GTP-Binding Proteins, EC; Magnesium, 7439-95-4; Manganese, 7439-96-5; NAD, 53-84-9; Pertussis Toxin, EC; Virulence Factors, Bordetella


  • Camargo, E.P., Kastelein, P., Roitman, I., Trypanosomatid Parasites of Plant (1990) Parasitol. Today, 6, pp. 22-25
  • Coso, O.A., Diaz Añel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Fraidenraich, D., Torres, H.N., Flawiá, M.M., Characterization of a Gi‐Protein from Trypanosoma cruzi epimastigote membranes (1992) Biochem. J., 287, pp. 443-446
  • Dollet, M., Plant diseases caused by flagellate protozoa (Phytomonas) (1984) Annual Review of Phytopathology, 22, pp. 115-132
  • Dollet, M., Cambrony, D., Gargani, D., Culture axenique in vitro de Phytomonas sp. (trypanosomatidae) d'Euphorbe, transmis par Stenocephalus agilis scap (coreide) (1982) C. R. Acad. Sci. III, 295, pp. 547-550
  • Eisenschlos, C., Paladini, A.A., Molina y Vedia, L., Torres, H.N., Flawiá, M.M., Evidence for the existence of an N‐type regulatory protein in Trypanosoma cruzi membranes (1986) Biochem. J., 237, pp. 913-917
  • Fernandes, A.P., Nelson, K., Beverley, S.M., Evolution of nuclear ribosomal RNAs in kinetoplatid protozoa: perspectives on the age and origins of parasitism (1993) Proc. Natl. Acad. Sci. (USA), 90, pp. 11608-11612
  • Goldsmith, P., Backlund, P.S., Rossiter, K., Carter, A., Milligan, G., Unson, C.G., Spiegel, A.M., Purification of heterotrimeric GTP binding proteins from brain (1988) Biochemistry, 27, pp. 7085-7090
  • Khyse‐Andersen, J., Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose (1984) Journal of Biochemical and Biophysical Methods, 10, pp. 203-209
  • Kornblihtt, A.R., Flawiá, M.M., Torres, H.N., Manganese ion dependent adenylate cyclase activity in rat testes: purification and properties (1981) Biochemistry, 20, pp. 1262-1267
  • Linse, K., Mandelkow, E.M., The GTP‐binding peptide of z‐tubulin (1988) J. Biol. Chem., 263, pp. 15205-15210
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Mumby, S.M., Kahn, R.A., Manning, D.R., Gilman, A.G., Antisera of designed specificity for subunits of guanine nucleotide‐binding regulatory proteins (1986) Proc. Natl. Acad. Sci. (USA), 83, pp. 265-269
  • Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D.B., Torres, H.N., Flawiá, M.M., Reconstitution of a light stimulated adenylate cyclase from retin and N. crassa preparations (1989) Eur. J. Biochem., 185, pp. 205-210
  • Muschietti, J.P., Martinetto, H.E., Coso, O., Farber, M.D., Torres, H.N., Flawiá, M.M., G‐Protein from Medicago sativa: functional association to photoreceptors (1993) Biochem. J., 291, pp. 383-388
  • Northup, J.K., Smigel, J.M., Gilman, A.G., The guanine nucleotide activating site of the regulatory component of adenylate cyclase. Identification by ligand binding (1982) J. Biol. Chem., 257, pp. 11416-11423
  • Rodbell, M., Metabolism of isolated fat cells. V. Preparations of “ghosts” and their properties, adenylate cyclase and other enzymes (1967) J. Biol. Chem., 242, pp. 5744-5750
  • Simonds, W.F., Goldsmith, P.K., Codina, J., Unson, C.G., Spiegel, A.M., Gi2 mediates α2‐adrenergic inhibition of adenylyl cyclase in platelet membranes: in situ identification with Gα C‐terminal antibodies (1989) Proc. Natl. Acad. Sci. (USA), 86, pp. 7809-7813
  • Solomon, Y., Londos, C., Rodbell, M., Highly sensitive adenylate cyclase assay (1974) Anal. Biochem., 58, pp. 541-548
  • Torruella, M., Flawiá, M.M., Eisenschlos, C.D., Molina, Vedia, L., Rubinstein, C.P., Torres, H.N., Trypanosoma cruzi adenylate cyclase activity Biochem (1986) J., 234, pp. 145-150
  • Waldo, G.L., Evans, T., Fraser, E.D., Northup, J.K., Martin, M.W., Hardern, T.K., Identification and purification from bovine brain of a guanine‐nucleotide binding protein distinct from Gs, Gi and Go (1987) Biochem. J., 246, pp. 431-439


---------- APA ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., Torres, H.N.,..., Flawia, M.M. (1995) . Adenyiyl Cyclase and G‐Proteins in Phytomonas. Journal of Eukaryotic Microbiology, 42(3), 257-260.
---------- CHICAGO ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al. "Adenyiyl Cyclase and G‐Proteins in Phytomonas" . Journal of Eukaryotic Microbiology 42, no. 3 (1995) : 257-260.
---------- MLA ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al. "Adenyiyl Cyclase and G‐Proteins in Phytomonas" . Journal of Eukaryotic Microbiology, vol. 42, no. 3, 1995, pp. 257-260.
---------- VANCOUVER ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al. Adenyiyl Cyclase and G‐Proteins in Phytomonas. J. Eukaryotic Microbiol. 1995;42(3):257-260.