Abstract:
ABSTRACT. Phytomonas sp. membranes have an adenylyl cyclase activity which is greater in the presence of Mn2+ than with Mg2+. The Mg2+ and Mn2+ activity ratio varies from one membrane preparation to another, suggesting that the adenylyl cyclase has a variable activation state. A [35S]GTP‐γ‐S‐binding activity with a Kd of 171 nM was detected in Phytomonas membranes. Incubation of these membranes with activated cholera or pertussis toxin and [adenylate 32P]NAD+ led to incorporation of radioactivity into bands of about 40–44 kDa. Crude membranes were electrophoresed on SDS‐polyacrylamide gels and analyzed, by Western blotting, with the 9188 anti‐αs antibody and the AS/7 antibody (anti‐α1, anti‐αi1, anti‐αi2). These procedures resulted in the identification of polypeptides of approximately 40–44 kDa. Phytomonas adenylyl cyclase could be activated by treatment of membrane preparations with cholera toxin, in the presence of NAD+, while similar treatment with pertussis toxin did not affect this enzyme activity. These studies indicate that in Phytomonas, adenylyl cyclase activity is coupled to an unknown receptor entity through Gαs, proteins. Copyright © 1995, Wiley Blackwell. All rights reserved
Registro:
Documento: |
Artículo
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Título: | Adenyiyl Cyclase and G‐Proteins in Phytomonas |
Autor: | Farber, M.D.; Montagna, A.E.; Paveto, C.; Dollet, M.; Sanchex-Moreno, M.; Osuna, A.; Torres, H.N.; Flawia, M.M. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina Départment des Cultures Perennes, Centre de Coopération Internationale en Recherche Agronomique pour le Développment, Montpellier, France Facultad de Ciencias, Universidad de Granada, Granada, Spain
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Palabras clave: | ADP‐ribosylation; cholera toxin; pertussis toxin; plant trypanosomatids; adenosine diphosphate; adenylate cyclase; adenylate cyclase toxin; bacterial toxin; Bordetella virulence factor; cholera toxin; divalent cation; guanosine triphosphate; heterotrimeric guanine nucleotide binding protein; magnesium; manganese; nicotinamide adenine dinucleotide; pertussis toxin; animal; article; cell membrane; cytology; drug effect; enzyme activation; enzymology; flagellate; metabolism; molecular weight; protein binding; Adenosine Diphosphate; Adenylate Cyclase; Adenylate Cyclase Toxin; Animals; Bacterial Toxins; Cations, Divalent; Cell Membrane; Cholera Toxin; Enzyme Activation; Guanosine Triphosphate; Heterotrimeric GTP-Binding Proteins; Magnesium; Manganese; Molecular Weight; NAD; Pertussis Toxin; Protein Binding; Trypanosomatina; Virulence Factors, Bordetella |
Año: | 1995
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Volumen: | 42
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Número: | 3
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Página de inicio: | 257
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Página de fin: | 260
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DOI: |
http://dx.doi.org/10.1111/j.1550-7408.1995.tb01576.x |
Título revista: | Journal of Eukaryotic Microbiology
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Título revista abreviado: | J. Eukaryotic Microbiol.
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ISSN: | 10665234
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CAS: | Adenosine Diphosphate, 58-64-0; Adenylate Cyclase Toxin; Adenylate Cyclase, EC 4.6.1.1; Bacterial Toxins; Cations, Divalent; Cholera Toxin, 9012-63-9; Guanosine Triphosphate, 86-01-1; Heterotrimeric GTP-Binding Proteins, EC 3.6.1.46; Magnesium, 7439-95-4; Manganese, 7439-96-5; NAD, 53-84-9; Pertussis Toxin, EC 2.4.2.31; Virulence Factors, Bordetella
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v42_n3_p257_Farber |
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Citas:
---------- APA ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., Torres, H.N.,..., Flawia, M.M.
(1995)
. Adenyiyl Cyclase and G‐Proteins in Phytomonas. Journal of Eukaryotic Microbiology, 42(3), 257-260.
http://dx.doi.org/10.1111/j.1550-7408.1995.tb01576.x---------- CHICAGO ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al.
"Adenyiyl Cyclase and G‐Proteins in Phytomonas"
. Journal of Eukaryotic Microbiology 42, no. 3
(1995) : 257-260.
http://dx.doi.org/10.1111/j.1550-7408.1995.tb01576.x---------- MLA ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al.
"Adenyiyl Cyclase and G‐Proteins in Phytomonas"
. Journal of Eukaryotic Microbiology, vol. 42, no. 3, 1995, pp. 257-260.
http://dx.doi.org/10.1111/j.1550-7408.1995.tb01576.x---------- VANCOUVER ----------
Farber, M.D., Montagna, A.E., Paveto, C., Dollet, M., Sanchex-Moreno, M., Osuna, A., et al. Adenyiyl Cyclase and G‐Proteins in Phytomonas. J. Eukaryotic Microbiol. 1995;42(3):257-260.
http://dx.doi.org/10.1111/j.1550-7408.1995.tb01576.x