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Abstract:

PCOS (polycystic ovary syndrome) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo- or amenorrhea, insulin resistance and anovulation. The aim of the present study was to evaluate if the balance between the ovarian expression of Bax (proapoptotic protein) and Bcl-2 (antiapoptotic protein) is altered in a PCOS model developed in rats by DHEA (dehydroepiandrosterone) administration. In addition, the ovarian morphology and the circulating progesterone levels were evaluated. Histological studies confirmed the presence of follicular cysts, atretic follicles and the absence of corpora lutea in the ovaries from the PCOS group and a significant decrease in circulating progesterone levels. Immunohistochemical studies showed that the expression of Bcl-2 and Bax were mainly localized in granulosa cells of AFs (antral follicles) in both groups. Bax expression was greater in preantral and AFs from PCOS ovarian sections than in the controls. In contrast, intense Bcl-2 immunostaining was observed in the control AFs, while Bcl-2 protein was either absent in PFs (preantral follicles) or weakly expressed in AFs from PCOS rats. These results were partially confirmed by Western studies. Data revealed that the ovarian level of Bcl-2 protein was lower in PCOS than in the control and that there were no differences in Bax ovarian levels between groups. However, Bax/Bcl-2 ratio was significantly higher in PCOS group than in the control group. In conclusion, an increase in ovarian apoptosis through an imbalance among the Bcl-2 family members may be involved in the transformation of growing follicles in cystic follicles in the ovaries from DHEA-induced PCOS rats. © The Author(s) Journal compilation © 2011 Portland Press Limited.

Registro:

Documento: Artículo
Título:Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats
Autor:Bas, D.; Abramovich, D.; Hernandez, F.; Tesone, M.
Filiación:Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Apoptotic protein; Dehydroepiandrosterone; Polycystic ovary syndrome; prasterone; progesterone; protein Bax; protein bcl 2; animal cell; animal experiment; animal model; animal tissue; apoptosis; article; cell structure; cellular distribution; controlled study; corpus luteum; female; follicle cyst; histopathology; immunohistochemistry; nonhuman; ovary follicle cell; ovary polycystic disease; pathogenesis; protein analysis; protein expression; protein function; protein localization; rat; Western blotting; Animals; bcl-2-Associated X Protein; Dehydroepiandrosterone; Female; Gene Expression Regulation; Ovary; Polycystic Ovary Syndrome; Progesterone; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley; Rattus
Año:2011
Volumen:35
Número:5
Página de inicio:423
Página de fin:429
DOI: http://dx.doi.org/10.1042/CBI20100542
Título revista:Cell Biology International
Título revista abreviado:Cell Biol. Int.
ISSN:10656995
CODEN:CBIIE
CAS:prasterone, 53-43-0; progesterone, 57-83-0; protein bcl 2, 219306-68-0; Dehydroepiandrosterone, 53-43-0; Progesterone, 57-83-0; Proto-Oncogene Proteins c-bcl-2; bcl-2-Associated X Protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v35_n5_p423_Bas

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Citas:

---------- APA ----------
Bas, D., Abramovich, D., Hernandez, F. & Tesone, M. (2011) . Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats. Cell Biology International, 35(5), 423-429.
http://dx.doi.org/10.1042/CBI20100542
---------- CHICAGO ----------
Bas, D., Abramovich, D., Hernandez, F., Tesone, M. "Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats" . Cell Biology International 35, no. 5 (2011) : 423-429.
http://dx.doi.org/10.1042/CBI20100542
---------- MLA ----------
Bas, D., Abramovich, D., Hernandez, F., Tesone, M. "Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats" . Cell Biology International, vol. 35, no. 5, 2011, pp. 423-429.
http://dx.doi.org/10.1042/CBI20100542
---------- VANCOUVER ----------
Bas, D., Abramovich, D., Hernandez, F., Tesone, M. Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats. Cell Biol. Int. 2011;35(5):423-429.
http://dx.doi.org/10.1042/CBI20100542