Artículo

Marinelli, L.J.; Piuri, M.; Hatfull, G.F."Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA" (2019) Methods in Molecular Biology. 1898:69-80
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Abstract:

We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA and a mutagenic substrate, which selects for only those cells that are competent to take up DNA. The cotransformation method, combined with the high rates of recombination obtained in M. smegmatis recombineering strains, allows for the efficient and rapid generation of bacteriophage mutants. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Registro:

Documento: Artículo
Título:Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA
Autor:Marinelli, L.J.; Piuri, M.; Hatfull, G.F.
Filiación:Division of Dermatology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United States
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina
Laboratorio “Bacteriófagos y Aplicaciones Biotecnológicas”, Departamento de Química Biológica, FCEyN, UBA, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Argentina
Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA, United States
Palabras clave:BRED; Electroporation; Mycobacteria; Mycobacteriophage; Recombineering; bacteriophage DNA; genomic DNA; oligonucleotide; Rac protein; recombinant protein; RecT protein; bacteriophage recombineering of electroporated DNA; bacterium culture; electroporation; genetic engineering; genetic manipulation; genetic recombination; methodology; mutant; mycobacteriophage; Mycobacterium smegmatis; natural science; nonhuman; polymerase chain reaction; prophage; recombineering
Año:2019
Volumen:1898
Página de inicio:69
Página de fin:80
DOI: http://dx.doi.org/10.1007/978-1-4939-8940-9_6
Handle:http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p69_Marinelli
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1898_n_p69_Marinelli

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Citas:

---------- APA ----------
Marinelli, L.J., Piuri, M. & Hatfull, G.F. (2019) . Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA. Methods in Molecular Biology, 1898, 69-80.
http://dx.doi.org/10.1007/978-1-4939-8940-9_6
---------- CHICAGO ----------
Marinelli, L.J., Piuri, M., Hatfull, G.F. "Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA" . Methods in Molecular Biology 1898 (2019) : 69-80.
http://dx.doi.org/10.1007/978-1-4939-8940-9_6
---------- MLA ----------
Marinelli, L.J., Piuri, M., Hatfull, G.F. "Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA" . Methods in Molecular Biology, vol. 1898, 2019, pp. 69-80.
http://dx.doi.org/10.1007/978-1-4939-8940-9_6
---------- VANCOUVER ----------
Marinelli, L.J., Piuri, M., Hatfull, G.F. Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA. Methods Mol. Biol. 2019;1898:69-80.
http://dx.doi.org/10.1007/978-1-4939-8940-9_6