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Abstract:

Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Registro:

Documento: Artículo
Título:Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
Autor:Piuri, M.; Hatfull, G.F.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina
Laboratorio “Bacteriófagos y Aplicaciones Biotecnológicas”, Departamento de Química Biológica, FCEyN, UBA, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Argentina
Department of Biological Sciences, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA, United States
Palabras clave:Antibiotics; DST; Fluoromycobacteriophage; Mycobacteria; Reporter phage; Resistance; carbenicillin; cycloheximide; ethambutol; ethionamide; isoniazid; kanamycin; rifampicin; streptomycin; tuberculostatic agent; antibiotic sensitivity; bacteriophage; flow cytometry; fluorescence microscopy; fluoromycobacteriophage; high throughput screening; mycobacteriosis; Mycobacterium; Mycobacterium smegmatis infection; nonhuman; tuberculosis
Año:2019
Volumen:1898
Página de inicio:27
Página de fin:36
DOI: http://dx.doi.org/10.1007/978-1-4939-8940-9_2
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
CAS:carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cycloheximide, 642-81-9, 66-81-9; ethambutol, 10054-05-4, 1070-11-7, 3577-94-4, 74-55-5; ethionamide, 536-33-4; isoniazid, 54-85-3, 62229-51-0, 65979-32-0; kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; rifampicin, 13292-46-1; streptomycin, 57-92-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1898_n_p27_Piuri

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Citas:

---------- APA ----------
Piuri, M. & Hatfull, G.F. (2019) . Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria. Methods in Molecular Biology, 1898, 27-36.
http://dx.doi.org/10.1007/978-1-4939-8940-9_2
---------- CHICAGO ----------
Piuri, M., Hatfull, G.F. "Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria" . Methods in Molecular Biology 1898 (2019) : 27-36.
http://dx.doi.org/10.1007/978-1-4939-8940-9_2
---------- MLA ----------
Piuri, M., Hatfull, G.F. "Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria" . Methods in Molecular Biology, vol. 1898, 2019, pp. 27-36.
http://dx.doi.org/10.1007/978-1-4939-8940-9_2
---------- VANCOUVER ----------
Piuri, M., Hatfull, G.F. Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria. Methods Mol. Biol. 2019;1898:27-36.
http://dx.doi.org/10.1007/978-1-4939-8940-9_2