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Jani, S.; Jackson, A.; Davies-Sala, C.; Chiem, K.; Soler-Bistué, A.; Zorreguieta, A.; Tolmasky, M.E. "Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules" (2018) Methods in Molecular Biology. 1737:89-98
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Abstract:

RNase P is a ribozyme consisting of a catalytic RNA molecule and, depending on the organism, one or more cofactor proteins. It was initially identified as the enzyme that mediates cleavage of precursor tRNAs at the 5′-end termini to generate the mature tRNAs. An important characteristic of RNase P is that its specificity depends on the structure rather than the sequence of the RNA substrate. Any RNA species that interacts with an antisense molecule (called external guide sequence, EGS) and forms the appropriate structure can be cleaved by RNase P. This property is the basis for EGS technology, an antisense methodology for inhibiting gene expression by eliciting RNase P-mediated cleavage of a target mRNA molecule. EGS technology is being developed to design therapies against a large variety of diseases. An essential milestone in developing EGSs as therapies is the assessment of the efficiency of antisense molecules to induce cleavage of the target mRNA and evaluate their effect in vivo. Here, we describe simple protocols to test the ability of EGSs to induce cleavage of a target mRNA in vitro and to induce a phenotypic change in growing cells. © 2018, Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules
Autor:Jani, S.; Jackson, A.; Davies-Sala, C.; Chiem, K.; Soler-Bistué, A.; Zorreguieta, A.; Tolmasky, M.E.
Filiación:Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA, United States
Fundación Instituto Leloir, IIBBA-CONICET, and FCEyN, University of Buenos Aires, Aires, Argentina
Palabras clave:Aminoglycoside; Antibiotic resistance; Antisense; Ribozyme; RNase P; antisense oligonucleotide; external guide sequence; messenger RNA; ribonuclease P; unclassified drug; antiinfective agent; antisense oligonucleotide; bacterial RNA; cell penetrating peptide; guide RNA; messenger RNA; ribonuclease P; RNA precursor; bacterial cell; bacterial growth; cell division; gene expression; in vitro study; phenotype; protein cleavage; protein targeting; antibiotic resistance; bacterium; drug effect; genetics; metabolism; Anti-Bacterial Agents; Bacteria; Cell-Penetrating Peptides; Drug Resistance, Bacterial; Oligoribonucleotides, Antisense; Ribonuclease P; RNA Precursors; RNA, Bacterial; RNA, Guide; RNA, Messenger
Año:2018
Volumen:1737
Página de inicio:89
Página de fin:98
DOI: http://dx.doi.org/10.1007/978-1-4939-7634-8_6
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
CAS:ribonuclease P, 71427-00-4; Anti-Bacterial Agents; Cell-Penetrating Peptides; Oligoribonucleotides, Antisense; Ribonuclease P; RNA Precursors; RNA, Bacterial; RNA, Guide; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1737_n_p89_Jani

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Citas:

---------- APA ----------
Jani, S., Jackson, A., Davies-Sala, C., Chiem, K., Soler-Bistué, A., Zorreguieta, A. & Tolmasky, M.E. (2018) . Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules. Methods in Molecular Biology, 1737, 89-98.
http://dx.doi.org/10.1007/978-1-4939-7634-8_6
---------- CHICAGO ----------
Jani, S., Jackson, A., Davies-Sala, C., Chiem, K., Soler-Bistué, A., Zorreguieta, A., et al. "Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules" . Methods in Molecular Biology 1737 (2018) : 89-98.
http://dx.doi.org/10.1007/978-1-4939-7634-8_6
---------- MLA ----------
Jani, S., Jackson, A., Davies-Sala, C., Chiem, K., Soler-Bistué, A., Zorreguieta, A., et al. "Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules" . Methods in Molecular Biology, vol. 1737, 2018, pp. 89-98.
http://dx.doi.org/10.1007/978-1-4939-7634-8_6
---------- VANCOUVER ----------
Jani, S., Jackson, A., Davies-Sala, C., Chiem, K., Soler-Bistué, A., Zorreguieta, A., et al. Assessment of external guide sequences’ (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules. Methods Mol. Biol. 2018;1737:89-98.
http://dx.doi.org/10.1007/978-1-4939-7634-8_6