Abstract:
Among the members of the Arenaviridae family, Junín virus and Lassa virus represent important human health threats generating annual outbreaks of severe human hemorrhagic fever (HF) in endemic areas of Argentina and Western Africa, respectively. Given the lack of a specific and safe chemotherapy, the search for effective antiviral compounds is a continuous demanding effort. During the last two decades, academic research studies originated important results identifying novel molecules to be considered for further in vivo characterization. This chapter summarizes experimental in vitro approaches used to determine the possible mechanism of action of these antiviral agents. © Springer Science+Business Media LLC 2018.
Registro:
Documento: |
Artículo
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Título: | Determining the virus life-cycle stage blocked by an antiviral |
Autor: | Sepúlveda, C.S.; García, C.C.; Damonte, E.B. |
Filiación: | Laboratorio de Estrategias Antivirales, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), UBA-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
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Palabras clave: | Antiviral compound; Antiviral mechanism; Arenaviruses; antivirus agent; complementary DNA; antivirus agent; Arenaviridae; cytopathogenic effect; cytotoxicity; cytotoxicity assay; disease severity; DNA synthesis; drug mechanism; EC50; endemic disease; hemorrhagic fever; high throughput screening; human; in vitro study; Junin virus; Lassa virus; life cycle stage; MTS assay; MTT assay; nonhuman; polyacrylamide gel electrophoresis; reverse transcription polymerase chain reaction; RNA synthesis; structure activity relation; virogenesis; virus assembly; virus attachment; virus culture; virus like agent; virus particle; virus release; virus replication; virus transcription; virus uncoating; Western blotting; Arenaviridae; drug effect; Hantaan virus; physiology; Antiviral Agents; Arenavirus; Hantaan virus |
Año: | 2018
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Volumen: | 1604
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Página de inicio: | 371
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Página de fin: | 392
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DOI: |
http://dx.doi.org/10.1007/978-1-4939-6981-4_28 |
Título revista: | Methods in Molecular Biology
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Título revista abreviado: | Methods Mol. Biol.
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ISSN: | 10643745
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CAS: | Antiviral Agents
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p371_Sepulveda |
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Citas:
---------- APA ----------
Sepúlveda, C.S., García, C.C. & Damonte, E.B.
(2018)
. Determining the virus life-cycle stage blocked by an antiviral. Methods in Molecular Biology, 1604, 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28---------- CHICAGO ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B.
"Determining the virus life-cycle stage blocked by an antiviral"
. Methods in Molecular Biology 1604
(2018) : 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28---------- MLA ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B.
"Determining the virus life-cycle stage blocked by an antiviral"
. Methods in Molecular Biology, vol. 1604, 2018, pp. 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28---------- VANCOUVER ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. Determining the virus life-cycle stage blocked by an antiviral. Methods Mol. Biol. 2018;1604:371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28