Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Among the members of the Arenaviridae family, Junín virus and Lassa virus represent important human health threats generating annual outbreaks of severe human hemorrhagic fever (HF) in endemic areas of Argentina and Western Africa, respectively. Given the lack of a specific and safe chemotherapy, the search for effective antiviral compounds is a continuous demanding effort. During the last two decades, academic research studies originated important results identifying novel molecules to be considered for further in vivo characterization. This chapter summarizes experimental in vitro approaches used to determine the possible mechanism of action of these antiviral agents. © Springer Science+Business Media LLC 2018.

Registro:

Documento: Artículo
Título:Determining the virus life-cycle stage blocked by an antiviral
Autor:Sepúlveda, C.S.; García, C.C.; Damonte, E.B.
Filiación:Laboratorio de Estrategias Antivirales, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), UBA-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Antiviral compound; Antiviral mechanism; Arenaviruses; antivirus agent; complementary DNA; antivirus agent; Arenaviridae; cytopathogenic effect; cytotoxicity; cytotoxicity assay; disease severity; DNA synthesis; drug mechanism; EC50; endemic disease; hemorrhagic fever; high throughput screening; human; in vitro study; Junin virus; Lassa virus; life cycle stage; MTS assay; MTT assay; nonhuman; polyacrylamide gel electrophoresis; reverse transcription polymerase chain reaction; RNA synthesis; structure activity relation; virogenesis; virus assembly; virus attachment; virus culture; virus like agent; virus particle; virus release; virus replication; virus transcription; virus uncoating; Western blotting; Arenaviridae; drug effect; Hantaan virus; physiology; Antiviral Agents; Arenavirus; Hantaan virus
Año:2018
Volumen:1604
Página de inicio:371
Página de fin:392
DOI: http://dx.doi.org/10.1007/978-1-4939-6981-4_28
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
CAS:Antiviral Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p371_Sepulveda

Referencias:

  • Enria, D.A., Briggiler, A.M., Sánchez, Z., Treatment of Argentine hemorrhagic fever (2008) Antivir Res, 78, pp. 132-139
  • McCormick, J.B., King, I.J., Webb, P.A., Scribner, C.L., Craven, R.B., Lassa fever. Effective therapy with ribavirin (1986) N Engl J Med, 314, pp. 20-26
  • Peters, C.J., Human infection with arenaviruses in the Americas. (2002) Curr Top Microbiol Immunol, 262, pp. 65-74
  • Fisher-Hoch, S.P., Ghorie, S., Parker, L., Huggins, J., Unexpected adverse reactions during a clinical trial in rural West Africa (1992) Antivir Res, 19, pp. 139-147
  • Larson, R.A., Dai, D., Hosack, V.T., Tan, Y., Bolken, T.C., Identification of a broad-spectrum arenavirus entry inhibitor (2008) J Virol, 82, pp. 10768-10775
  • Lee, A.M., Rojek, J.M., Spiropoulou, C.F., Gundersen, A.T., Jin, W., Unique small molecule entry inhibitors of hemorrhagic fever arenaviruses (2008) J Biol Chem, 283, pp. 18734-18742
  • Capul, A.A., De La Torre, J.C., A cell-based luciferase assay amenable to high-throughput screening of arenavirus budding (2008) Virology, 382, pp. 107-114
  • Lee, A.M., Pasquato, A., Kunz, S., Novel approaches in anti-arenaviral drug development (2011) Virology, 411, pp. 163-169
  • Sepúlveda, C.S., Fascio, M.L., Mazzucco, M.B., Palacios, M.L., Pellón, R.F., Synthesis and evaluation of N-substituted acridones as antiviral agents against hemorrhagic fever viruses (2008) Antivir Chem Chemother, 19, pp. 41-47
  • Sepúlveda, C.S., García, C.C., Fascio, M.L., D’Accorso, N.B., Docampo Palacios, M.L., Inhibition of Junín virus RNA synthesis by an antiviral acridone derivative (2012) Antivir Res, 96, pp. 16-22
  • García, C.C., Sepúlveda, C.S., Damonte, E.B., Novel therapeutic targets for arenavirus hemorrhagic fevers (2011) Future Virol, 6, pp. 27-44
  • Linero, F.N., Scolaro, L.A., Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro (2009) Virus Res, 145, pp. 166-170
  • De Guerrero, L.B., Weissenbacher, M.C., Parodi, A.S., Inmunización contra la fiebre hemorrágica Argentina con una cepa atenuada de virus Junín (1969) Medicina (Buenos Aires), 29, pp. 1-5
  • Maiztegui, J.I., McKee, K.T., Barrera Oro, J.G., Harrison, L.H., Gibbs, P.H., Protective efficacy of a live attenuated vaccine against Argentina hemorrhagic fever (1998) J Infect Dis, 177, pp. 277-283
  • Contigiani, M.S., Sabattini, M.S., Virulencia diferencial de cepas de virus Junín por marcadores biológicos en ratones y cobayos (1977) Medicina (Buenos Aires), 37, pp. 244-251
  • Albariño, C.G., Bergeron, E., Erickson, B.R., Khristova, M.L., Rollin, P.E., Nichol, S.T., Efficient reverse genetics generation of infectious Junin viruses differing in glycoprotein processing (2009) J Virol, 83, pp. 5606-5614
  • Emonet, S.F., Seregin, A.V., Yun, N.E., Poussard, A.L., Walker, A.G., Rescue from cloned cDNAs and in vivo characterization of recombinant pathogenic Romero and live-attenuated candid #1 strains of Junin virus, the causative agent of Argentine hemorrhagic fever disease (2011) J Virol, 85, pp. 1473-1483
  • Carnec, X., Baize, S., Reynard, S., Diancourt, L., Caro, V., Lassa virus nucleoprotein mutants generated by reverse genetics induce a robust type I interferon response in human dendritic cells and macrophages (2011) J Virol, 85, pp. 12093-12097
  • Sanchez, A., Pifat, D.Y., Kenyon, R.H., Peters, C.J., McCormick, J.B., Kiley, M.P., Junin virus monoclonal antibodies: Characterization and cross-reactivity with other arenaviruses (1989) J Gen Virol, 70, pp. 1125-1132
  • Bolken, T.C., Laquerre, S., Zhang, Y., Bailey, T.R., Pevear, D.C., Identification and characterization of potent small molecule inhibitor of hemorrhagic fever new world arenavirus (2006) Antivir Res, 69, pp. 86-97
  • York, J., Dai, D., Amberg, S., Nunberg, J.H., PH-induced activation of arenavirus membrane fusion is antagonized by small-molecule inhibitors (2008) J Virol, 82, pp. 10932-10939
  • Cashman, K.A., Smith, M.A., Twenhafel, N.A., Larson, R.A., Jones, K.F., Allen, R., Evaluation of Lassa antiviral compound ST-193 in a Guinea pig model (2011) Antivir Res, 90, pp. 70-79
  • Thomas, C.J., Casquilho-Gray, H.E., York, J., Decamp, D.L., Dai, D., A specific interaction of small molecule entry inhibitors with the envelope glycoprotein complex of the Junín hemorrhagic fever arenavirus (2011) J Biol Chem, 286, pp. 6192-6200
  • Whitby, L.R., Lee, A.M., Kunz, S., Oldstone, M., Boger, D.L., Characterization of Lassa virus cell entry inhibitors: Determination of the active enantiomer by asymmetric synthesis (2009) Bioorg Med Chem Lett, 19, pp. 3771-3774
  • Lee, A.M., Rojek, J.M., Gundersen, A.T., Ströher, U., Juteau, J.M., Inhibition of cellular entry of lymphocytic choriomeningitis virus by amphipathic DNA polymers (2008) Virology, 372, pp. 107-117
  • Wolf, M.C., Freiberg, A.N., Zhang, T., Akyol-Ataman, Z., Grock, A., A broad-spectrum antiviral targeting entry of enveloped viruses (2010) Proc Natl Acad Sci U S A, 107, pp. 3157-3162
  • York, J., Berry, J.D., Ströher, U., Li, Q., Feldmann, H., An antibody directed against the fusion peptide of Junín virus envelope glycoprotein GPC inhibits pH-induced membrane fusion (2010) J Virol, 84, pp. 6119-6129
  • García, C.C., Candurra, N.A., Damonte, E.B., Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds (2000) Antivir Chem Chemother, 11, pp. 231-237
  • García, C.C., Candurra, N.A., Damonte, E.B., Differential inhibitory action of two azoic compounds against arenaviruses (2003) Int J Antimicrob Agents, 21, pp. 319-324
  • García, C.C., Candurra, N.A., Damonte, E.B., Mode of inactivation of arenaviruses by disulfide based compounds (2002) Antivir Res, 55, pp. 437-446
  • García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A., Damonte, E.B., Characterization of Junín virus particles inactivated by a zincfinger-reactive compound (2009) Virus Res, 143, pp. 106-113
  • García, C.C., Djavani, M., Topisirovic, I., Borden, K.L., Salvato, M.S., Damonte, E.B., Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger reactive compounds (2006) J Gen Virol, 87, pp. 1217-1228
  • Sepúlveda, C.S., García, C.C., Damonte, E.B., Inhibition of arenavirus infection by thiuram and aromatic disulfides (2010) Antivir Res, 87, pp. 329-337
  • Sepúlveda, C.S., García, C.C., Levingston Macleod, J.M., López, N., Damonte, E.B., Targeting of arenavirus RNA synthesis by a carboxamide-derivatized aromatic disulfide with virucidal activity (2013) Plos One, 8, pp. e81251
  • Ortiz-Riaño, E., Ngo, N., Devito, S., Eggink, D., Munger, J., Inhibition of arenavirus by A3, a pyrimidine biosynthesis inhibitor (2014) J Virol, 88, pp. 878-889
  • Wachsman, M.B., López, E., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral effect of brassinosteroids against herpes virus and arenavirus (2000) Antivir Chem Chemother, 11, pp. 71-77
  • Castilla, V., Larzábal, M., Aguirre Sgalippa, N., Wachsman, M.B., Coto, C.E., Antiviral mode of action of a synthetic brassinosteroid against Junín virus replication (2005) Antivir Res, 68, pp. 88-95
  • Acosta, E.G., Bruttomesso, A.C., Bisceglia, J.A., Wachsman, M.B., Galagovsky, L.R., Castilla, V., Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junín virus replication in vitro (2008) Virus Res, 135, pp. 203-212
  • Barradas, J.S., Errea, M.I., D'accorso, N.B., Sepúlveda, C.S., Talarico, L.B., Damonte, E.B., Synthesis and antiviral activity of azoles obtained from carbohydrates (2008) Carbohydr Res, 343, pp. 2468-2474
  • Barradas, J.S., Errea, M.I., D'accorso, N.B., Sepúlveda, C.S., Damonte, E.B., Imidazo[2,1-b]thiazole carbohydrate derivatives: Synthesis and antiviral activity against Junin virus, agent of Argentine hemorrhagic fever (2011) Eur J Med Chem, 46, pp. 259-264
  • Gowen, B.B., Wong, M.H., Jung, K.H., Sanders, A.B., Mendenhall, M., In vitro and in vivo activities of T-705 against arenavirus and bunyavirus infections (2007) Antimicrob Agents Chemother, 51, pp. 3168-3176
  • Gowen, B.B., Smee, D.F., Wong, M.-H., Hall, J.O., Jung, K.H., Treatment of late stage disease in a model of arenaviral hemorrhagic fever: T-705 efficacy and reduced toxicity suggests an alternative to ribavirin (2008) Plos One, pp. e3725
  • Müller, S., Günther, S., Broad-spectrum antiviral activity of small interfering RNA targeting the conserved RNA termini of Lassa virus (2007) Antimicrob Agents Chemother, 51, pp. 2215-2218
  • Artuso, M.C., Ellenberg, P.C., Scolaro, L.A., Damonte, E.B., García, C.C., Inhibition of Junín virus replication by small interfering RNAs (2009) Antivir Res, 84, pp. 31-37
  • Lu, J., Han, Z., Liu, Y., Liu, W., Lee, M.S., A host-oriented inhibitor of Junin Argentine hemorrhagic fever virus egress (2014) J Virol, 88, pp. 4736-4743
  • Neumann, B.W., Bederka, L.H., Stein, D.A., Ting, J., Moulton, H.M., Buchmeier, M.J., Development of peptide-conjugated morpholino oligomers as pan-arenavirus inhibitors (2011) Antimicrob Agents Chemother, 55, pp. 4631-4638
  • Rathbun, J., Droniou, M.E., Damoiseaux, R., Haworth, K.G., Henley, J.E., Novel arenavirus entry inhibitors discovered by using a minigenome rescue system for high-throughput drug screening (2015) J Virol, 89, pp. 8428-8443
  • Vázquez-Calvo, A., Martín-Acebes, M.A., Sáiz, J.C., Ngo, N., Sobrino, F., De La Torre, J.C., Inhibition of the multiplication of the prototypic arenavirus LCMV by valproic acid (2013) Antivir Res, 99, pp. 172-179
  • Pasquato, A., Rochat, C., Burri, D.J., Pasqual, G., De La Torre, J.C., Kunz, S., Evaluation of the anti-arenaviral activity of the subtilisin kexin isozyme-1/site-1 protease inhibitor PF-429242 (2012) Virology, 423, pp. 14-22

Citas:

---------- APA ----------
Sepúlveda, C.S., García, C.C. & Damonte, E.B. (2018) . Determining the virus life-cycle stage blocked by an antiviral. Methods in Molecular Biology, 1604, 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28
---------- CHICAGO ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. "Determining the virus life-cycle stage blocked by an antiviral" . Methods in Molecular Biology 1604 (2018) : 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28
---------- MLA ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. "Determining the virus life-cycle stage blocked by an antiviral" . Methods in Molecular Biology, vol. 1604, 2018, pp. 371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28
---------- VANCOUVER ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. Determining the virus life-cycle stage blocked by an antiviral. Methods Mol. Biol. 2018;1604:371-392.
http://dx.doi.org/10.1007/978-1-4939-6981-4_28