Abstract:
Identification of cell moieties involved in viral binding and internalization is essential since their expression would render a cell susceptible. Further steps that allow the uncoating of the viral particle at the right subcellular localization have been intensively studied. These “entry” steps could determine cell permissiveness and often define tissue and host tropism. Therefore applying the right and, when possible, straightforward experimental approaches would shorten avenues to the complete knowledge of this first and key step of any viral life cycle. Mammarenaviruses are enveloped viruses that enter the host cell via receptor-mediated endocytosis. In this chapter we present a set of customized experimental approaches and tools that were used to describe the entry of Junín virus (JUNV), and other New World mammarenavirus members, into mammalian cells. © Springer Science+Business Media LLC 2018.
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Documento: |
Artículo
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Título: | Entry studies of new world arenaviruses |
Autor: | Martínez, M.G.; Forlenza, M.B.; Candurra, N.A.; Cordo, S.M. |
Filiación: | Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, United States Laboratorio de Bioquímica y Biología del virus Junín, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Intendente Güiraldes 2160, Buenos Aires, 1428, Argentina
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Palabras clave: | Endocytosis pathways; Receptor recognition; Virus; 3T3 cell line; cellular distribution; endocytosis; genetic transfection; host cell; Junin virus; life cycle; Mammarenavirus; Moloney murine leukemia virus; New World arenavirus; nonhuman; nucleotide sequence; pH; tropism; Vero cell line; virus attachment; virus entry; virus envelope; virus particle; virus strain; animal; Arenaviridae; human; New World arenavirus; pathogenicity; physiology; Animals; Arenaviridae; Arenaviruses, New World; Endocytosis; Humans |
Año: | 2018
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Volumen: | 1604
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Página de inicio: | 113
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Página de fin: | 133
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DOI: |
http://dx.doi.org/10.1007/978-1-4939-6981-4_8 |
Título revista: | Methods in Molecular Biology
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Título revista abreviado: | Methods Mol. Biol.
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ISSN: | 10643745
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p113_Martinez |
Referencias:
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Citas:
---------- APA ----------
Martínez, M.G., Forlenza, M.B., Candurra, N.A. & Cordo, S.M.
(2018)
. Entry studies of new world arenaviruses. Methods in Molecular Biology, 1604, 113-133.
http://dx.doi.org/10.1007/978-1-4939-6981-4_8---------- CHICAGO ----------
Martínez, M.G., Forlenza, M.B., Candurra, N.A., Cordo, S.M.
"Entry studies of new world arenaviruses"
. Methods in Molecular Biology 1604
(2018) : 113-133.
http://dx.doi.org/10.1007/978-1-4939-6981-4_8---------- MLA ----------
Martínez, M.G., Forlenza, M.B., Candurra, N.A., Cordo, S.M.
"Entry studies of new world arenaviruses"
. Methods in Molecular Biology, vol. 1604, 2018, pp. 113-133.
http://dx.doi.org/10.1007/978-1-4939-6981-4_8---------- VANCOUVER ----------
Martínez, M.G., Forlenza, M.B., Candurra, N.A., Cordo, S.M. Entry studies of new world arenaviruses. Methods Mol. Biol. 2018;1604:113-133.
http://dx.doi.org/10.1007/978-1-4939-6981-4_8