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Abstract:

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241–1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2′,7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015.

Registro:

Documento: Artículo
Título:Improved ROS measurement in root hair cells
Autor:Juárez, S.P.D.; Mangano, S.; Estevez, J.M.
Filiación:Laboratorio de Fisiología y Biología Molecular, IFIByNE (CONICET), FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina
Palabras clave:2′,7-Dihidro dicloro fluorescein; Root hair; ROS; reactive oxygen metabolite; 2,7-dihydrodichlorofluorescein diacetate; Arabidopsis protein; fluorescein derivative; fluorescent dye; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide phosphate oxidase; Arabidopsis thaliana; Article; confocal microscopy; image analysis; measurement; nonhuman; plant cell; plant growth; root hair; Arabidopsis; chemistry; cytology; cytosol; enzymology; metabolism; plant cell; plant root; Arabidopsis; Arabidopsis Proteins; Cytosol; Fluoresceins; Fluorescent Dyes; NADPH Oxidase; Plant Cells; Plant Roots; Reactive Oxygen Species
Año:2015
Volumen:1242
Página de inicio:67
Página de fin:71
DOI: http://dx.doi.org/10.1007/978-1-4939-1902-4_6
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
CAS:reduced nicotinamide adenine dinucleotide phosphate oxidase, 9032-22-8; 2,7-dihydrodichlorofluorescein diacetate; Arabidopsis Proteins; Fluoresceins; Fluorescent Dyes; NADPH Oxidase; Reactive Oxygen Species
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p67_Juarez

Referencias:

  • Suzuki, N., Miller, G., Morales, J., Shulaev, V., Torres, M., Mittler, R., Respiratory burst oxidases: The engines of ROS signaling (2011) Plant Biol, 14, pp. 691-699
  • Roppolo, D., De Rybel, B., Tendon, V., Pfister, A., Alassimone, J., Vermeer, J., Yamazaki, M., Geldner, N., A novel protein family mediates Casparian strip formation in the endodermis (2011) Nature, 473, pp. 380-384
  • Takeda, S., Gapper, C., Kaya, H., Bell, E., Kuchitsu, K., Dolan, L., Local positive feedback regulation determines cell shape in root hair cells (2008) Science, 319, pp. 1241-1244
  • Potocký, M., Jones, M., Bezvoda, R., Smirnoff, N., Zárský, V., Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth (2007) New Phytol, 174, pp. 742-751
  • Foreman, J., Demidchik, V., Bothwell, J., Mylona, P., Miedema, H., Torres, M., Linstead, P., Jones, J., Reactive oxygen species produced by NADPH oxidase regulate plant cell growth (2003) Nature, 422, pp. 442-446
  • Karlsson, M., Kurz, T., Brunk, U., Nilsson, S., Frennesson, C., What does the commonly used DCF test for oxidative stress really show? (2010) Biochem J, 428, pp. 183-190

Citas:

---------- APA ----------
Juárez, S.P.D., Mangano, S. & Estevez, J.M. (2015) . Improved ROS measurement in root hair cells. Methods in Molecular Biology, 1242, 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6
---------- CHICAGO ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M. "Improved ROS measurement in root hair cells" . Methods in Molecular Biology 1242 (2015) : 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6
---------- MLA ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M. "Improved ROS measurement in root hair cells" . Methods in Molecular Biology, vol. 1242, 2015, pp. 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6
---------- VANCOUVER ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M. Improved ROS measurement in root hair cells. Methods Mol. Biol. 2015;1242:67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6