Artículo

Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" (2015) Methods in Molecular Biology. 1242:41-47
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Abstract:

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015.

Registro:

Documento: Artículo
Título:Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
Autor:Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Héctor Torres (INGEBI-CONICET), Buenos Aires, Argentina
Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina
Palabras clave:Arabidopsis pollen; Cell expansion; Pollen tube growth; calcium ion; reactive oxygen metabolite; Arabidopsis protein; bacterial DNA; T-DNA; Arabidopsis thaliana; Article; assay; controlled study; gene insertion; growth regulation; in vitro study; mutant; nonhuman; phenotype; plant growth; pollen germination; pollen tube; pollen tube growth; Arabidopsis; botany; cell polarity; embryology; gene expression regulation; genetics; germination; growth, development and aging; mutation; physiology; procedures; Arabidopsis; Arabidopsis thaliana; Arabidopsis; Arabidopsis Proteins; Botany; Cell Polarity; DNA, Bacterial; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Germination; Mutation; Pollen Tube
Año:2015
Volumen:1242
Página de inicio:41
Página de fin:47
DOI: http://dx.doi.org/10.1007/978-1-4939-1902-4_3
Título revista:Methods in Molecular Biology
Título revista abreviado:Methods Mol. Biol.
ISSN:10643745
CAS:calcium ion, 14127-61-8; Arabidopsis Proteins; DNA, Bacterial; T-DNA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p41_Borassi

Referencias:

  • Cheung, A.Y., Hen-Ming, W., Structural and signaling networks for the polar cell growth machinery in pollen tubes (2008) Annu Rev Plant Biol, 59, pp. 547-572
  • Cole, R.A., Fowler, J.E., Polarized growth: Maintaining focus on the tip (2006) Curr Opin Plant Biol, 9, pp. 579-588
  • Chebli, Y., Geitmann, A., Mechanical principles governing pollen tube growth (2007) Funct Plant Sci Biotechnol, 1, pp. 232-245
  • Boavida, L.C., McCormick, S., Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana (2007) Plant J, 52, pp. 570-582

Citas:

---------- APA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J. & Estevez, J.M. (2015) . Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Methods in Molecular Biology, 1242, 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- CHICAGO ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Methods in Molecular Biology 1242 (2015) : 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- MLA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Methods in Molecular Biology, vol. 1242, 2015, pp. 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- VANCOUVER ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Methods Mol. Biol. 2015;1242:41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3