Artículo
Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" (2015) Methods in Molecular Biology. 1242:41-47
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Abstract:
Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015.
Registro:
| Documento: | Artículo |
| Título: | Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| Autor: | Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M. |
| Filiación: | Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Héctor Torres (INGEBI-CONICET), Buenos Aires, Argentina Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina |
| Palabras clave: | Arabidopsis pollen; Cell expansion; Pollen tube growth; calcium ion; reactive oxygen metabolite; Arabidopsis protein; bacterial DNA; T-DNA; Arabidopsis thaliana; Article; assay; controlled study; gene insertion; growth regulation; in vitro study; mutant; nonhuman; phenotype; plant growth; pollen germination; pollen tube; pollen tube growth; Arabidopsis; botany; cell polarity; embryology; gene expression regulation; genetics; germination; growth, development and aging; mutation; physiology; procedures; Arabidopsis; Arabidopsis thaliana; Arabidopsis; Arabidopsis Proteins; Botany; Cell Polarity; DNA, Bacterial; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Germination; Mutation; Pollen Tube |
| Año: | 2015 |
| Volumen: | 1242 |
| Página de inicio: | 41 |
| Página de fin: | 47 |
| DOI: | http://dx.doi.org/10.1007/978-1-4939-1902-4_3 |
| Título revista: | Methods in Molecular Biology |
| Título revista abreviado: | Methods Mol. Biol. |
| ISSN: | 10643745 |
| CAS: | calcium ion, 14127-61-8; Arabidopsis Proteins; DNA, Bacterial; T-DNA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p41_Borassi |
Referencias:
- Cheung, A.Y., Hen-Ming, W., Structural and signaling networks for the polar cell growth machinery in pollen tubes (2008) Annu Rev Plant Biol, 59, pp. 547-572
- Cole, R.A., Fowler, J.E., Polarized growth: Maintaining focus on the tip (2006) Curr Opin Plant Biol, 9, pp. 579-588
- Chebli, Y., Geitmann, A., Mechanical principles governing pollen tube growth (2007) Funct Plant Sci Biotechnol, 1, pp. 232-245
- Boavida, L.C., McCormick, S., Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana (2007) Plant J, 52, pp. 570-582
Citas:
---------- APA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J. & Estevez, J.M. (2015) . Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Methods in Molecular Biology, 1242, 41-47.http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- CHICAGO ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Methods in Molecular Biology 1242 (2015) : 41-47.http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- MLA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Methods in Molecular Biology, vol. 1242, 2015, pp. 41-47.http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- VANCOUVER ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Methods Mol. Biol. 2015;1242:41-47.http://dx.doi.org/10.1007/978-1-4939-1902-4_3
