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Abstract:

The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. To characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume. © 2016 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
Autor:Plaul, S.E.; Pastor, R.; Díaz, A.O.; Barbeito, C.G.
Filiación:Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada, Facultad de Ciencias Veterinarias, UNLP. La Plata, Buenos Aires, Argentina
Departamento de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, UNL. Esperanza, Santa Fe, Argentina
Laboratorio de Histología e Histoquímica, Departamento de Biología, Instituto de Investigaciones Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, CONICET, UNdMP. Mar del Plata, Buenos Aires, Argentina
Palabras clave:Air-breathing organ; Na+K+-ATPase; Neotropical catfish; PCNA; adenosine triphosphatase (potassium sodium); cycline; animal; animal structures; catfish; chemistry; cytology; female; immunohistochemistry; intestine mucosa; male; metabolism; physiology; ultrastructure; Animal Structures; Animals; Catfishes; Female; Immunohistochemistry; Intestinal Mucosa; Male; Proliferating Cell Nuclear Antigen; Sodium-Potassium-Exchanging ATPase
Año:2016
Volumen:79
Número:3
Página de inicio:140
Página de fin:148
DOI: http://dx.doi.org/10.1002/jemt.22614
Título revista:Microscopy Research and Technique
Título revista abreviado:Microsc. Res. Tech.
ISSN:1059910X
CODEN:MRTEE
CAS:adenosine triphosphatase (potassium sodium); Proliferating Cell Nuclear Antigen; Sodium-Potassium-Exchanging ATPase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v79_n3_p140_Plaul

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Citas:

---------- APA ----------
Plaul, S.E., Pastor, R., Díaz, A.O. & Barbeito, C.G. (2016) . Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine. Microscopy Research and Technique, 79(3), 140-148.
http://dx.doi.org/10.1002/jemt.22614
---------- CHICAGO ----------
Plaul, S.E., Pastor, R., Díaz, A.O., Barbeito, C.G. "Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine" . Microscopy Research and Technique 79, no. 3 (2016) : 140-148.
http://dx.doi.org/10.1002/jemt.22614
---------- MLA ----------
Plaul, S.E., Pastor, R., Díaz, A.O., Barbeito, C.G. "Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine" . Microscopy Research and Technique, vol. 79, no. 3, 2016, pp. 140-148.
http://dx.doi.org/10.1002/jemt.22614
---------- VANCOUVER ----------
Plaul, S.E., Pastor, R., Díaz, A.O., Barbeito, C.G. Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine. Microsc. Res. Tech. 2016;79(3):140-148.
http://dx.doi.org/10.1002/jemt.22614