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Abstract:

Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolymerization and that very low laser power is required. This procedure allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets, or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and a standard laser scanning microscope was used to demonstrate that it is possible to use equipment readily available in several laboratories. We show that fluorescent structure with less than 10 nm in height may be used as references in fluorescence microscopy allowing a smooth environment for cell growth. Different dyes were tested and the conditions for one-photon polymerization were outlined. © 2009 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Single photon fluorescent microlithography for live-cell imaging
Autor:Kunik, D.; Aramendia, P.F.; Martínez, O.E.
Filiación:Dpto. de Física, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Departamento de Química Inorgánica, Analítica y Química Física, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
Palabras clave:Colocalization; Lithography; Microfluidics; Microscopy; Photopolymerization; Animals; Cells, Cultured; Laser Therapy, Low-Level; Melanophores; Microscopy, Confocal; Microscopy, Fluorescence; Polyurethanes; Urethane; Xenopus
Año:2010
Volumen:73
Número:1
Página de inicio:20
Página de fin:26
DOI: http://dx.doi.org/10.1002/jemt.20748
Título revista:Microscopy Research and Technique
Título revista abreviado:Microsc. Res. Tech.
ISSN:1059910X
CODEN:MRTEE
CAS:Polyurethanes; Urethane, 51-79-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v73_n1_p20_Kunik

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Citas:

---------- APA ----------
Kunik, D., Aramendia, P.F. & Martínez, O.E. (2010) . Single photon fluorescent microlithography for live-cell imaging. Microscopy Research and Technique, 73(1), 20-26.
http://dx.doi.org/10.1002/jemt.20748
---------- CHICAGO ----------
Kunik, D., Aramendia, P.F., Martínez, O.E. "Single photon fluorescent microlithography for live-cell imaging" . Microscopy Research and Technique 73, no. 1 (2010) : 20-26.
http://dx.doi.org/10.1002/jemt.20748
---------- MLA ----------
Kunik, D., Aramendia, P.F., Martínez, O.E. "Single photon fluorescent microlithography for live-cell imaging" . Microscopy Research and Technique, vol. 73, no. 1, 2010, pp. 20-26.
http://dx.doi.org/10.1002/jemt.20748
---------- VANCOUVER ----------
Kunik, D., Aramendia, P.F., Martínez, O.E. Single photon fluorescent microlithography for live-cell imaging. Microsc. Res. Tech. 2010;73(1):20-26.
http://dx.doi.org/10.1002/jemt.20748