Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The aim of this study was to analyze whether di(2-ethylhexyl) phthalate (DEHP), a Sertoli and Leydig cell toxicant, is able to induce alterations in the expression of testicular gap and tight junction proteins. DEHP was administered by gavage (1 g/5 mL corn oil/kg body weight/ day) to 25-day-old male Sprague-Dawley rats for 2 days (DEHP-27d) and control rats were treated with corn-oil vehicle for 2 days (C-27d); animals were killed 24 h after the last treatment. Testes of DEHP-27d rats showed different degrees of germ cell sloughing of seminiferous tubules (ST). No alterations of the blood testis barrier (BTB) by lanthanum tracer study were observed. ST of DEHP-27d rats showed a milder immunofluorescence and more restricted expression of connexin-43 (Cx43) in the adluminal and basal compartment compared to C-27d. In DEHP-27d rats, we found a discontinuous immunofluorescent (IF) pattern for zonula occludens (ZO-1), contrasting with the continuous IF profile observed in C-27d, and a delocalization of claudin-11. A decrease in Cx43 and ZO-1 and no changes in occludin expression were detected by Western blot in the testes of DEHP-27d rats. Results from 57-day-old rats treated with DEHP for 2 days and held for 30 days without treatment showed that the alterations in protein expression induced by DEHP are reversible. However, a delay of spermatogenesis compared to C-57d rats, occurred. Data demonstrated that DEHP does not impair BTB permeability but induces germ cell sloughing that might respond to a down regulation of Cx43 and ZO-1 that alters cell junction proteins. © 2009 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
Autor:Sobarzo, C.M.; Lustig, L.; Ponzio, R.; Suescun, M.O.; Denduchis, B.
Filiación:Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 P10, C 1121 ABG, Buenos Aires, Argentina
Facultad de Ciencias Exactas Y Naturales, Instituto Multidisciplinario de Biología Celular, Universidad de La Plata, cc 403, 1900, La Plata, Prov. Bs.As., Argentina
Idioma: Inglés
Palabras clave:Claudin-11; Connexin 43; Occludin; Reproductive toxicant; Zonula occludens-1; claudin 11; lanthanum; occludin; phthalic acid bis(2 ethylhexyl) ester; protein ZO1; testosterone; animal experiment; animal tissue; article; blood testis barrier; body weight; controlled study; gap junction; histopathology; immunofluorescence; Leydig cell; male; nonhuman; priority journal; protein expression; rat; Sertoli cell; spermatogenesis; testis; testosterone blood level; tight junction; Western blotting; Animals; Connexins; Diethylhexyl Phthalate; Gap Junctions; Leydig Cells; Male; Rats; Rats, Sprague-Dawley; Seminiferous Tubules; Sertoli Cells; Testis; Tight Junctions; Animalia; Rattus; Zea mays
Año:2009
Volumen:72
Número:11
Página de inicio:868
Página de fin:877
DOI: http://dx.doi.org/10.1002/jemt.20741
Título revista:Microscopy Research and Technique
Título revista abreviado:Microsc. Res. Tech.
ISSN:1059910X
CODEN:MRTEE
CAS:lanthanum, 16096-89-2, 7439-91-0; occludin, 176304-61-3; phthalic acid bis(2 ethylhexyl) ester, 117-81-7; testosterone, 58-22-0; Connexins; Diethylhexyl Phthalate, 117-81-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v72_n11_p868_Sobarzo

Referencias:

  • Akingbemi, B.T., Youker, R.T., Sottas, C.M., Ge, R., Katz, E., Klinefelter, G.R., Zirkin, B.R., Hardy, M.P., Modulation of rat Leydig cell steroidogenic function by di(2-ethylhexyl)phthalate (2001) Biology of Reproduction, 65 (4), pp. 1252-1259
  • Albro, P.W., The biochemical toxicology of di(2-ethylhexyl) phthalate and related phthalates: Testicular atrophy and hepatocarcinogenesis (1987) Rev Biochem Toxicol, 8, pp. 73-119
  • Batias, C., Siffroi, J.-P., Fenichel, P., Pointis, G., Segretain, D., Connexin43 gene expression and regulation in the rodent seminiferous epithelium (2000) Journal of Histochemistry and Cytochemistry, 48 (6), pp. 793-805
  • Boekelheide, K., Johnson, K.J., Richburg, J.H., Sertoli cell toxicants (2005) Sertoli Cell Biology, pp. 345-382. , Skinner MK, Griswold MD, editors. Elsevier: New York
  • Brehm, R., Ruttinger, C., Fischer, P., Gashaw, I., Winterhager, E., Kliesch, S., Bohle, R.M., Bergmann, M., Transition from preinvasive carcinoma in situ to seminoma is accompanied by a reduction of connexin 43 expression in sertoli cells and germ cells (2006) Neoplasia, 8 (6), pp. 499-509. , http://docserver.ingentaconnect.com/deliver/connect/neo/15228002/v8n6/s9. pdf?expires=1152517334&id=30398771&titleid=3829&accname= Guest+User&checksum=2AB9DA47C264DFD2D1EC0819CAB94955, DOI 10.1593/neo.05847
  • Byers, S., Graham, R., Dai, H.N., Hoxter, B., Development of Sertoli cell junctional specializations and the distribution of the tight-junction- Associated protein ZO-1 in the mouse testis (1991) Am J Anat, 191, pp. 35-47
  • Cavicchia, J.C., Sacerdote, F.L., Ortiz, L., The human blood-testis barrier in impaired spermatogenesis (1996) Ultrastructural Pathology, 20 (3), pp. 211-218
  • Cyr, D.G., Hermo, L., Egenberger, N., Mertineit, C., Trasler, J.M., Laird, D.W., Cellular immunolocalization of occludin during embryonic and postnatal development of the mouse testis and epididymis (1999) Endocrinology, 140 (8), pp. 3815-3825
  • Defamie, N., Mograbi, B., Roger, C., Cronier, L., Malassine, A., Brucker-Davis, F., Fenichel, P., Pointis, G., Disruption of gap junctional intercellular communication by lindane is associated with aberrant localization of connexin43 and zonula occludens-1 in 42GPA9 Sertoli cells (2001) Carcinogenesis, 22 (9), pp. 1537-1542
  • Defamie, N., Berthaut, I., Mograbi, B., Chevallier, D., Dadoune, J.-P., Fenichel, P., Segretain, D., Pointis, G., Impaired gap junction connexin43 in Sertoli cells of patients with secretory azoospermia: A marker of undifferentiated Sertoli cells (2003) Laboratory Investigation, 83 (3), pp. 449-456
  • Dufau, M.L., Mendelson, C., Catt, K.J., A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: Testosterone production by dispersed Leydig cells (1974) J Clin Endocrinol, 2, pp. 860-863
  • Fink, C., Weigel, R., Hembes, T., Lauke-Wettwer, H., Kliesch, S., Bergmann, M., Brehm, R.H., Altered expression of ZO-1 and ZO-2 in sertoli cells and loss of blood-testis barrier integrity in testicular carcinoma in situ (2006) Neoplasia, 8 (12), pp. 1019-1027. , http://www.neoplasia.com/pdf/manuscript/neo06559.pdf, DOI 10.1593/neo.06559
  • Fiorini, C., Tilloy-Ellul, A., Chevalier, S., Charuel, C., Pointis, G., Sertoli cell junctional proteins as early targets for different classes of reproductive toxicants (2004) Reproductive Toxicology, 18 (3), pp. 413-421. , DOI 10.1016/j.reprotox.2004.01.002, PII S0890623804000164
  • Förster, C., Tight junctions and the modulation of barrier function in disease (2008) Histochem Cell Biol, 130, pp. 55-70
  • Ge, R.-S., Chen, G.-R., Tanrikut, C., Hardy, M.P., Phthalate ester toxicity in Leydig cells: Developmental timing and dosage considerations (2007) Reproductive Toxicology, 23 (3), pp. 366-373. , DOI 10.1016/j.reprotox.2006.12.006, PII S0890623806003029
  • Gow, A., Southwood, C.M., Li, J.S., Pariali, M., Riordan, G.P., Brodie, S.E., Danias, J., Lazzarini, R.A., CNS Myelin and sertoli cell tight junction strands are absent in OSP/claudin-11 null mice (1999) Cell, 99 (6), pp. 649-659
  • Hartsock, A., Nelson, W.J., Adherens and tight junctions: Structure, function and connections to the actin cytoskeleton (2008) Biochim Biophys Acta, 1778, pp. 660-669
  • Howdeshell, K.L., Rider, C.V., Wilson, V.S., Gray Jr., L.E., Mechanisms of action of phthalate esters, individually and in combination, to induce abnormal reproductive development in male laboratory rats (2008) Environ Res, 108, pp. 168-176
  • Kang, K.S., Lee, Y.S., Kim, H.S., Di-(2-ethylhexyl) phthalate-induced cell proliferation is involved in the inhibition of gap junctional intercellular communication and blockage of apoptosis in mouse Sertoli cells (2002) J Toxicol Environ Health a, 65, pp. 447-459
  • Kavlock, R., Boekelheide, K., Chapin, R., Cunningham, M., Faustman, E., Foster, P., Golub, M., Zacharewski, T., NTP center for the evaluation of risk to human reproduction: Phthalates expert panel report on the reproductive and developmental toxicity of di(2-ethylhexyl) phthalate (2002) Reprod Toxicol, 16, pp. 529-653
  • Hyun, J.K., Byung, M.L., Estimated exposure to phthalates in cosmetics and risk assessment (2004) Journal of Toxicology and Environmental Health - Part a, 67 (23-24), pp. 1901-1914. , DOI 10.1080/15287390490513300
  • Kotula-Balak, M., Hejmej, A., Sadowska, J., Bilinska, B., Connexin 43 expression in human and mouse testes with impaired spermatogenesis (2007) Eur J Histochem, 51, pp. 261-268
  • Lee, N.P.Y., Leung, K.-W., Wo, J.Y., Tam, P.-C., Yeung, W.S.B., Luk, J.M., Blockage of testicular connexins induced apoptosis in rat seminiferous epithelium (2006) Apoptosis, 11 (7), pp. 1215-1229. , DOI 10.1007/s10495-006-6981-2
  • Lefevre, A., Saez, J.M., Finaz, C., HCG responsiveness of purified Leydig cells from immature and mature rats (1983) Hormone Research, 17 (2), pp. 114-120
  • Lui, W.-Y., Mruk, D., Lee, W.M., Cheng, C.Y., Sertoli cell tight junction dynamics: Their regulation during spermatogenesis (2003) Biology of Reproduction, 68 (4), pp. 1087-1097. , DOI 10.1095/biolreprod.102.010371
  • Morita, K., Sasaki, H., Fujimoto, K., Furuse, M., Tsukita, S., Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis (1999) Journal of Cell Biology, 145 (3), pp. 579-588. , DOI 10.1083/jcb.145.3.579
  • Mruk, D.D., Cheng, C.Y., Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis (2004) Endocrinol Rev, 25, pp. 747-806
  • Pelletier, R.M., The distribution of connexin 43 is associated with the germ cell differentiation and with the modulation of the Sertoli cell junctional barrier in continual (guinea pig) and seasonal breeder s (mink) testes (1995) J Androl, 16, pp. 400-409
  • Pelletier, R.-M., Okawara, Y., Vitale, M.L., Anderson, J.M., Differential distribution of the tight-junction-associated protein ZO-1 isoforms α+ and α- in guinea pig sertoli cells: A possible association with F-actin and G-actin (1997) Biology of Reproduction, 57 (2), pp. 367-376. , DOI 10.1095/biolreprod57.2.367
  • Plum, A., Hallas, G., Magin, T., Dombrowski, F., Hagendorff, A., Schumacher, B., Wolpert, C., Willecke, K., Unique and shared functions of different connexins in mice (2000) Curr Biol, 10, pp. 1083-1091
  • Pointis, G., Fiorini, C., Defamie, N., Segretain, D., Gap junctional communication in the male reproductive system (2005) Biochimica et Biophysica Acta - Biomembranes, 1719 (1-2), pp. 102-116. , DOI 10.1016/j.bbamem.2005.09.017, PII S000527360500297X, The Connexins Part III
  • Richburg, J.H., Johnson, K.J., Schoenfeld, H.A., Meistrich, M.L., Dix, D.J., Defining the cellular and molecular mechanisms of toxicant action in the testis (2002) Toxicology Letters, 135 (3), pp. 167-183. , DOI 10.1016/S0378-4274(02)00254-0, PII S0378427402002540
  • Roscoe, W.A., Barr, K.J., Mhawi, A.A., Pomerantz, D.K., Kidder, G.M., Failure of spermatogenesis in mice lacking connexin43 (2001) Biology of Reproduction, 65 (3), pp. 829-838
  • Russell, L.D., Ettlin, R.A., Sinha Hikim, A.P., Clegg, E.J., (1990) Histological and Histopatological Evaluation of the Testis, pp. 1-52. , Clearwater, FL: Cache River
  • Saitou, M., Furuse, M., Sasaki, H., Schulzke, J.D., Fromm, M., Takano, H., Noda, T., Tsukita, S., Complex phenotype of mice lacking occludin, a component of tight junction strands (2000) Mol Biol Cell, 11, pp. 4131-4142
  • Sobarzo, C.M., Lustig, L., Ponzio, R., Denduchis, B., Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis (2006) Reproductive Toxicology, 22 (1), pp. 77-86. , DOI 10.1016/j.reprotox.2006.02.004, PII S0890623806000591
  • Sridharan, S., Simon, L., Meling, D.D., Cyr, D.G., Gulstein, D.E., Fishman, G.I., Guillou, F., Cooke, P.S., Proliferation of adult sertoli cells following conditional knockout of the gap junctional protein GJA1 (connexin 43) in mice (2007) Biology of Reproduction, 76 (5), pp. 804-812. , DOI 10.1095/biolreprod.106.059212
  • Suescun, M.O., Campo, S., Rivarola, M.A., Testosterone, dihydrotestosterone, and zinc concentrations in human testis and epididymis (1981) Archives of Andrology, 7 (4), pp. 297-303
  • Suescun, M.O., Calandra, R.S., Lustig, L., Alterations of testicular function after induced autoimmune orchitis in rats (1994) Journal of Andrology, 15 (5), pp. 442-448
  • Tan, I.P., Roy, C., Saez, J.C., Saez, C.G., Paul, D.L., Risley, M.S., Regulated assembly of connexin33 and connexin43 into rat Sertoli cell gap junctions (1996) Biology of Reproduction, 54 (6), pp. 1300-1310
  • Wei, C.-J., Xu, X., Lo, C.W., Connexins and cell signaling in development and disease (2004) Annual Review of Cell and Developmental Biology, 20, pp. 811-838. , DOI 10.1146/annurev.cellbio.19.111301.144309
  • Wong, C.-H., Mruk, D.D., Lui, W.-Y., Cheng, C.Y., Regulation of blood-testis barrier dynamics: An in vivo study (2004) Journal of Cell Science, 117 (5), pp. 783-798. , DOI 10.1242/jcs.00900
  • Zhang, Y.-H., Lin, L., Liu, Z.-W., Jiang, X.-Z., Chen, B.-H., Disruption effects of monophthalate exposures on inter-sertoli tight junction in a two-compartment culture model (2008) Environmental Toxicology, 23 (3), pp. 302-308. , DOI 10.1002/tox.20343

Citas:

---------- APA ----------
Sobarzo, C.M., Lustig, L., Ponzio, R., Suescun, M.O. & Denduchis, B. (2009) . Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats. Microscopy Research and Technique, 72(11), 868-877.
http://dx.doi.org/10.1002/jemt.20741
---------- CHICAGO ----------
Sobarzo, C.M., Lustig, L., Ponzio, R., Suescun, M.O., Denduchis, B. "Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats" . Microscopy Research and Technique 72, no. 11 (2009) : 868-877.
http://dx.doi.org/10.1002/jemt.20741
---------- MLA ----------
Sobarzo, C.M., Lustig, L., Ponzio, R., Suescun, M.O., Denduchis, B. "Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats" . Microscopy Research and Technique, vol. 72, no. 11, 2009, pp. 868-877.
http://dx.doi.org/10.1002/jemt.20741
---------- VANCOUVER ----------
Sobarzo, C.M., Lustig, L., Ponzio, R., Suescun, M.O., Denduchis, B. Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats. Microsc. Res. Tech. 2009;72(11):868-877.
http://dx.doi.org/10.1002/jemt.20741