Artículo
Costantino, S.; Heinze, K.G.; Martínez, O.E.; De Koninck, P.; Wiseman, P.W. "Two-photon fluorescent microlithography for live-cell imaging" (2005) Microscopy Research and Technique. 68(5):272-276
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Abstract:
Fluorescent dyes added to UV-cure resins allow the rapid fabrication of fluorescent micropatterns on standard glass coverslips by two-photon optical lithography. We use this lithographic method to tailor fiduciary markers, focal references, and calibration tools, for fluorescence and laser scanning microscopy. Fluorescent microlithography provides spatial landmarks to quantify molecular transport, cell growth and migration, and to compensate for focal drift during time-lapse imaging. We show that the fluorescent patterned microstructures are biocompatible with cultures of mammalian cell lines and hippocampal neurons. Furthermore, the high-relief topology of the lithographed substrates is utilized as a mold for poly(dimethylsiloxane) stamps to create protein patterns by microcontact printing, representing an alternative to the current etching techniques. We present two different applications of such protein patterns for localizing cell adhesion and guidance of neunte outgrowth. © 2005 Wiley-Liss, Inc.
Registro:
| Documento: | Artículo |
| Título: | Two-photon fluorescent microlithography for live-cell imaging |
| Autor: | Costantino, S.; Heinze, K.G.; Martínez, O.E.; De Koninck, P.; Wiseman, P.W. |
| Filiación: | Department of Physics, McGill University, Montréal, Qué., Canada Laboratorio de Electrónica Cuántica, Departamento de Física, Universidad de Buenos Aires, Buenos Aires, Argentina Département de Biochimie et de Microbiologie, Université Laval, Centre de Recherche Université Laval Robert-Giffard, Qué., Canada Department of Chemistry, McGill University, Montréal, Qué., Canada Department of Physics, McGill University, 3600 University Street, Montréal, Qué. H3A2T8, Canada |
| Palabras clave: | Fluorescent fiduciary marks; Fluorescent lithography; Microcontact printing; Nonlinear optical lithography; dimeticone; fluorescent dye; glass; protein; resin; analytic method; animal cell; article; biocompatibility; calibration; cell adhesion; cell culture; cell growth; cell migration; fluorescence; hippocampus; laser microscopy; mammal cell; nerve fiber growth; newborn; nonhuman; priority journal; quantitative analysis; rat; two photon fluorescent microlithography; Animals; Animals, Newborn; Cells, Cultured; CHO Cells; Cricetinae; Dimethylpolysiloxanes; Fibronectins; Fluorescent Dyes; Green Fluorescent Proteins; Hippocampus; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Microscopy, Fluorescence; Neurons; Photons; Polymers; Rats; Animalia; Mammalia |
| Año: | 2005 |
| Volumen: | 68 |
| Número: | 5 |
| Página de inicio: | 272 |
| Página de fin: | 276 |
| DOI: | http://dx.doi.org/10.1002/jemt.20247 |
| Título revista: | Microscopy Research and Technique |
| Título revista abreviado: | Microsc. Res. Tech. |
| ISSN: | 1059910X |
| CODEN: | MRTEE |
| CAS: | dimeticone, 32028-95-8, 68248-27-1, 9004-73-3, 9006-65-9; protein, 67254-75-5; Dimethylpolysiloxanes; Fibronectins; Fluorescent Dyes; Green Fluorescent Proteins, 147336-22-9; Polymers |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v68_n5_p272_Costantino |
Referencias:
- Bernard, A., Renault, J.P., Michel, B., Bosshard, H.R., Delamarche, E., Microcontact printing of proteins (2000) Adv Mater, 12, pp. 1067-1070
- Chen, C.S., Mrksich, M., Huang, S., Whitesides, G.M., Ingber, D.E., Geometric control of cell life and death (1997) Science, 276, pp. 1425-1428
- Cumpston, B.H., Ananthavel, S.P., Barlow, S., Dyer, D.L., Ehrlich, J.E., Erskine, L.L., Heikal, A.A., Perry, J.W., Two-photon polymerization initiators for three-dimensional optical data storage and microfabrication (1999) Nature, 398, pp. 51-54
- Galajda, P., Ormos, P., Complex micromachines produced and driven by light (2001) Appl Phys Lett, 78, pp. 249-251
- Hudmon, A., LeBel, E., Roy, H., Schulman, H., Waxham, N., De Koninck, P., A mechanism for Ca2+/calmodulin-dependent protein kinase II clustering at synaptic and non-synaptic sites based on self-association (2005) J Neurosci, 25, pp. 6971-6983
- Knight, E., Laukitisa, C., Akhtarb, N., Hotchinb, N.A., Edlunde, M., Horwitz, A.R., Visualizing muscle cell migration in situ (2000) Curr Biol, 10, pp. 576-585
- Lehnert, D., Wehrle-Haller, B., David, C., Weiland, U., Ballestrem, C., Imhof, B.A., Bastmeyer, M., Cell behaviour on micropatterned substrata: Limits of extracellular matrix geometry for spreading and adhesion (2004) J Cell Sci, 117, pp. 41-52
- Maruo, S.O., Nakamura, O., Kawata, K., Three-dimensional microfabrication with two-photon absorbed photopolymerization (1997) Opt Lett, 22, pp. 132-134
- Ridley, A.J., Schwartz, M.A., Burridge, K., Firtel, R.A., Ginsberg, M.H., Borisy, G., Parsons, J.T., Horwitz, A.R., Cell migration: Integrating signals from front to back (2003) Science, 302, pp. 1704-1709
- Shah, S.V., Cleveland, D.W., Slow axonal transport: Fast motors in the slow lane (2002) Curr Opin Cell Biol, 14, pp. 58-62
- Singhvi, R., Kumar, A., Lopez, G.P., Stephanopoulos, G.N., Wang, D.I., Whitesides, G.M., Ingber, D.E., Engineering cell shape and function (1994) Science, 264, pp. 696-698
- Sun, H.B., Tanaka, T., Takada, K., Kawata, S., Two-photon photopolymerization and diagnosis of three-dimensional microstructures containing fluorescent dyes (2001) Appl Phys Lett, 79, pp. 1411-1413
- Thomas, C.H., Collier, J.H., Sfeir, C.S., Healy, K.E., Engineering gene expression and protein synthesis by modulation of nuclear shape (2002) Proc Natl Acad Sci USA, 99, pp. 1972-1977
Citas:
---------- APA ----------
Costantino, S., Heinze, K.G., Martínez, O.E., De Koninck, P. & Wiseman, P.W. (2005) . Two-photon fluorescent microlithography for live-cell imaging. Microscopy Research and Technique, 68(5), 272-276.http://dx.doi.org/10.1002/jemt.20247
---------- CHICAGO ----------
Costantino, S., Heinze, K.G., Martínez, O.E., De Koninck, P., Wiseman, P.W. "Two-photon fluorescent microlithography for live-cell imaging" . Microscopy Research and Technique 68, no. 5 (2005) : 272-276.http://dx.doi.org/10.1002/jemt.20247
---------- MLA ----------
Costantino, S., Heinze, K.G., Martínez, O.E., De Koninck, P., Wiseman, P.W. "Two-photon fluorescent microlithography for live-cell imaging" . Microscopy Research and Technique, vol. 68, no. 5, 2005, pp. 272-276.http://dx.doi.org/10.1002/jemt.20247
---------- VANCOUVER ----------
Costantino, S., Heinze, K.G., Martínez, O.E., De Koninck, P., Wiseman, P.W. Two-photon fluorescent microlithography for live-cell imaging. Microsc. Res. Tech. 2005;68(5):272-276.http://dx.doi.org/10.1002/jemt.20247
