Abstract:
In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc.
Registro:
Documento: |
Artículo
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Título: | Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells |
Autor: | Grecco, H.E.; Lidke, K.A.; Heintzmann, R.; Lidke, D.S.; Spagnuolo, C.; Martinez, O.E.; Jares-Erijman, E.A.; Jovin, T.M. |
Filiación: | Departamento de Física, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina Department of Molecular Biology, Max Planck Inst. Biophysical Chem., D-37070 Göttingen, Germany Departamento de Quimica Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina Dept. of Physics, FCEN, University of Buenos Aires, C1428EHA Buenos Aires, Argentina Dept. of Molecular Biology, Max Planck Inst. Biophysical Chem., D-37070 Göttingen, Germany
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Palabras clave: | Fluorescence microscopy; Growth factor receptors; Nanoparticles; epidermal growth factor receptor; green fluorescent protein; growth factor receptor; hybrid protein; nanoparticle; quantum dot; streptavidin; transferrin receptor; fluorescent dye; nanomaterial; transferrin; anisotropy; article; cancer cell culture; confocal laser microscopy; fluorescence; fluorescence lifetime imaging microscopy; fluorescence microscopy; fluorescence resonance energy transfer; human; human cell; imaging system; irradiation; microscopy; multiphoton microscopy; photon; priority journal; signal transduction; squamous cell carcinoma; two photon excitation microscopy; fluorescence microscopy; fluorescence resonance energy transfer; metabolism; tumor cell line; Anisotropy; Cell Line, Tumor; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; Microscopy, Fluorescence; Nanostructures; Photons; Quantum Dots; Receptors, Growth Factor; Signal Transduction; Transferrin |
Año: | 2004
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Volumen: | 65
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Número: | 4-5
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Página de inicio: | 169
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Página de fin: | 179
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DOI: |
http://dx.doi.org/10.1002/jemt.20129 |
Título revista: | Microscopy Research and Technique
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Título revista abreviado: | Microsc. Res. Tech.
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ISSN: | 1059910X
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CODEN: | MRTEE
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CAS: | streptavidin, 9013-20-1; transferrin, 82030-93-1; Fluorescent Dyes; Receptors, Growth Factor; Transferrin, 11096-37-0
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v65_n4-5_p169_Grecco |
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Citas:
---------- APA ----------
Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., Jares-Erijman, E.A.,..., Jovin, T.M.
(2004)
. Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microscopy Research and Technique, 65(4-5), 169-179.
http://dx.doi.org/10.1002/jemt.20129---------- CHICAGO ----------
Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., et al.
"Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells"
. Microscopy Research and Technique 65, no. 4-5
(2004) : 169-179.
http://dx.doi.org/10.1002/jemt.20129---------- MLA ----------
Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., et al.
"Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells"
. Microscopy Research and Technique, vol. 65, no. 4-5, 2004, pp. 169-179.
http://dx.doi.org/10.1002/jemt.20129---------- VANCOUVER ----------
Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., et al. Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microsc. Res. Tech. 2004;65(4-5):169-179.
http://dx.doi.org/10.1002/jemt.20129