Artículo

Thomas, M.G.; Tosar, L.J.M.; Loschi, M.; Pasquini, J.M.; Correale, J.; Kindler, S.; Boccaccio, G.L. "Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes" (2005) Molecular Biology of the Cell. 16(1):405-420
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Abstract:

Staufen is a conserved double-stranded RNA-binding protein required for mRNA localization in Drosophila oocytes and embryos. The mammalian homologues Staufen 1 and Staufen 2 have been implicated in dendritic RNA targeting in neurons. Here we show that in rodent oligodendrocytes, these two proteins are present in two independent sets of RNA granules located at the distal myelinating processes. A third kind of RNA granules lacks Staufen and contains major myelin mRNAs. Myelin Staufen granules associate with microfilaments and microtubules, and their subcellular distribution is affected by polysome-disrupting drugs. Under oxidative stress, both Staufen 1 and Staufen 2 are recruited into stress granules (SGs), which are stress-induced organelles containing transiently silenced messengers. Staufen SGs contain the poly(A)-binding protein (PABP), the RNA-binding proteins HuR and TIAR, and small but not large ribosomal subunits. Staufen recruitment into perinuclear SGs is paralleled by a similar change in the overall localization of polyadenylated RNA. Under the same conditions, the distribution of recently transcribed and exported mRNAs is not affected. Our results indicate that Staufen 1 and Staufen 2 are novel and ubiquitous SG components and suggest that Staufen RNPs are involved in repositioning of most polysomal mRNAs, but not of recently synthesized transcripts, during the stress response.

Registro:

Documento: Artículo
Título:Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes
Autor:Thomas, M.G.; Tosar, L.J.M.; Loschi, M.; Pasquini, J.M.; Correale, J.; Kindler, S.; Boccaccio, G.L.
Filiación:Fundación Instituto Leloir, IIB Fac. de Cie. Exactas y Naturales, University of Buenos Aires, Buenos Aires, Argentina
Inst. de Quim. y Fisicoquimica Biol., Facultad de Farmacia y Bioquimica, University of Buenos Aires, Buenos Aires, Argentina
Department of Neurology, Inst. Invest. Neurologicas R. Carrea, FLENI, Buenos Aires, Argentina
Center for Molecular Neurobiology, Univ. Hospital Hamburg-Eppendorf, University of Hamburg, Hamburg, Germany
Palabras clave:HuR protein; messenger RNA; myelin; polyadenylic acid binding protein; protein staufen 1; protein staufen 2; protein tiar; RNA binding protein; unclassified drug; animal cell; article; cell granule; microfilament; microtubule; nonhuman; nucleotide sequence; oligodendroglia; polysome; priority journal; protein targeting; rat; RNA transport; stress; Alternative Splicing; Animals; Animals, Newborn; Biological Transport; Blotting, Western; Brain; Cloning, Molecular; Computer Simulation; Cytoplasm; In Situ Hybridization, Fluorescence; Microfilaments; Microscopy, Confocal; Microscopy, Fluorescence; Microtubules; Models, Genetic; Myelin Sheath; Oligodendroglia; Oxidative Stress; Polyribosomes; Protein Structure, Tertiary; Rats; Rats, Sprague-Dawley; Ribonucleases; Ribosomes; RNA; RNA, Double-Stranded; RNA, Messenger; RNA-Binding Proteins; Animalia; Mammalia; Rodentia
Año:2005
Volumen:16
Número:1
Página de inicio:405
Página de fin:420
DOI: http://dx.doi.org/10.1091/mbc.E04-06-0516
Título revista:Molecular Biology of the Cell
Título revista abreviado:Mol. Biol. Cell
ISSN:10591524
CODEN:MBCEE
CAS:Ribonucleases, EC 3.1.-; RNA, 63231-63-0; RNA, Double-Stranded; RNA, Messenger; RNA-Binding Proteins; Stau protein, rat; Stau2 protein, rat
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v16_n1_p405_Thomas

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Citas:

---------- APA ----------
Thomas, M.G., Tosar, L.J.M., Loschi, M., Pasquini, J.M., Correale, J., Kindler, S. & Boccaccio, G.L. (2005) . Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Molecular Biology of the Cell, 16(1), 405-420.
http://dx.doi.org/10.1091/mbc.E04-06-0516
---------- CHICAGO ----------
Thomas, M.G., Tosar, L.J.M., Loschi, M., Pasquini, J.M., Correale, J., Kindler, S., et al. "Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes" . Molecular Biology of the Cell 16, no. 1 (2005) : 405-420.
http://dx.doi.org/10.1091/mbc.E04-06-0516
---------- MLA ----------
Thomas, M.G., Tosar, L.J.M., Loschi, M., Pasquini, J.M., Correale, J., Kindler, S., et al. "Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes" . Molecular Biology of the Cell, vol. 16, no. 1, 2005, pp. 405-420.
http://dx.doi.org/10.1091/mbc.E04-06-0516
---------- VANCOUVER ----------
Thomas, M.G., Tosar, L.J.M., Loschi, M., Pasquini, J.M., Correale, J., Kindler, S., et al. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol. Biol. Cell. 2005;16(1):405-420.
http://dx.doi.org/10.1091/mbc.E04-06-0516