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Abstract:

The sequence of events during protein and lipid deposition has been examined in the developing endosperm of Myrsine laetevirens using light and electron microscopy and chemical analyses. At 10-20 d after pollination (DAP), plastids contain starch grains and phytoferritin deposits in a crystalline arrangement. Phytoferritin disappears 30 DAP, when lipid bodies are formed via secretion from smooth endoplasmic reticulum. Rough endoplasmic reticulum and Golgi stacks are present in all stages of development but are particularly evident during protein deposition. Protein bodies arise from preexistent vacuoles or derive directly from Golgi vesicles. Deposition of the crystalloid proteins occurs between 40 and 50 DAP; the matrix proteins appear at 60-70 DAP. Analysis of fatty acids by gas-liquid chromatography revealed that lipid accumulation follows a sigmoidal pattern. During the first analyzed stage (10 DAP), the fatty acid composition corresponds to structural membrane lipids. From 20 to 60 DAP, the dominant fatty acids were C18:2, C18:1, and C16:0, in a relative proportion almost similar to that of the storage lipids from mature seeds. In the mature endosperm, matrix and buffer-soluble proteins resolve into seven bands under reducing conditions and into six bands under nonreducing conditions; some of these bands were glycosylated. The crystalloid and the insoluble protein fraction resolve into five bands under both nonreducing and reducing conditions; no band is glycosylated.

Registro:

Documento: Artículo
Título:Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies
Autor:Otegui, M.; Maldonado, S.; Lima, C.; De Lederkremer, R.M.
Filiación:Laboratorio de Morfologia Vegetal, Facultad de Cie. Naturales y Museo, Universidad Nacional de La Plata, Paseo del Bosque s/n, 1900 la Plata, Argentina
Instituto de Recurs. Biologicos, INTA, 1712 Castelar, Argentina
Depto. de Quimica Orgánica, Facultad de Cie. Exact. y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Endosperm development; Lipids; Myrsine laetevirens; Phytoferritin; Proteins; Myrsine laetevirens
Año:1999
Volumen:160
Número:3
Página de inicio:501
Página de fin:509
DOI: http://dx.doi.org/10.1086/314151
Título revista:International Journal of Plant Sciences
Título revista abreviado:Int. J. Plant Sci.
ISSN:10585893
CODEN:IPLSE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10585893_v160_n3_p501_Otegui

Referencias:

  • Andrews, J.E., Ohlrogge, J., Fatty acid and lipid biosynthesis and degradation (1990) Plant Physiology, Biochemistry and Molecular Biology, pp. 339-352. , in DT Dennis, DH Turpin, eds. Longman, London
  • Bewley, J.D., Greenwood, J.S., Protein storage and utilization in seeds (1990) Plant Physiology, Biochemistry and Molecular Biology, pp. 453-469. , in DT Dennis, DH Turpin, eds. Longman, London
  • Browse, J., Somerville, C., Glycerolipid synthesis: Biochemistry and regulation (1991) Annu Rev Plant Physiol Plant Mol Biol, 42, pp. 467-506
  • Craig, S., Goodchild, D.J., Hardham, A., Structural aspects of protein accumulation in developing pea cotyledons. I. Qualitative and quantitative changes in parenchyma cell vacuoles (1979) Aust J Plant Physiol, 6, pp. 81-98
  • Craig, S., Goodchild, D.J., Miller, C., Structural aspects of protein accumulation in developing pea cotyledons. II. Tridimensional reconstructions of vacuoles and protein bodies from serial sections (1980) Aust J Plant Physiol, 7, pp. 329-337
  • Heder, R.G., O'Brien, T., Plant microtechniques: Some principles and new methods (1968) Am J Bot, 55, pp. 123-142
  • Frey-Wyssling, A., Grieshaber, E., Mühlethales, K., Origin of spherosomes in plant cells (1963) J Ultrastruct Res, 8, pp. 506-516
  • Fulcher, R., Fluorescence microscopy of cereals (1982) Food Microstruct, 1, pp. 167-175
  • Gahan, P., (1984) Plant Hisrochemistry and Cytochemistry: An Introduction, , Academic Press, New York
  • Gifford, D.J., Greenwood, J.S., Bewley, J.D., Deposition of matrix and crystalloid storage proteins during protein body development in the endosperm of Ricinus communis L. cv. Hale seeds (1982) Plant Physiol, 69, pp. 1471-1478
  • Gurr, M.I., The biosynthesis of triacylglycerols (1980) Lipids: Structure and Function, pp. 205-248. , in PK Stumpf, ed. Academic Press, New York
  • Harrison, P.M., Arosio, P., The ferritins: Molecular properties, iron storage function and cellular regulation (1996) Biochim Biophys Acta, 1275, pp. 161-203
  • Korn, E.D., Wright, P.L., Macromolecular composition of an Amoeba plasma membrane (1973) J Biol Chem, 248, pp. 439-447
  • Lott, J., Protein bodies in seeds (1980) Nord J Bot, 1, pp. 421-432
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Madrigal, R., Spencer, G., Plattner, R., Smith, C.R., Alkyl-and alkenylresorcinols in Rapanea laetevirens seed lipids (1977) Lipids, 12, pp. 402-406
  • Miquel, M., Browse, J., Lipid biosynthesis in developing seeds (1995) Seed Development and Germination, pp. 169-193. , in J Kigel, G Galili, eds. Dekker, New York
  • Murray, D.R., (1984) Seed Physiology, 1. , Development. Academic Press, London
  • Ohlrogge, J.B., Jaworski, J.G., Regulation of fatty acid synthesis (1997) Annu Rev Plant Physiol Plant Mol Biol, 48, pp. 109-136
  • Otegui, M., Lima, C., Maldonado, S., De Lederkremer, R.M., Histological and chemical characterization of Myrsine laetevirens seed (1998) Int J Plant Sci, 159, pp. 762-772
  • Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I.Cellularization and deposition of cell wall storage carbohydrates (1999) Int J Plant Sci, 160, pp. 491-500
  • Rest, J.A., Vaughan, J.G., The development of protein and oil bodies in the seed of Sinapsis alba L (1972) Planta, 105, pp. 245-262
  • Robards, A.W., Humpherson, P.G., Phytoferritin in plastids of the cambial zone of willow (1967) Planta, 76, pp. 169-178
  • Segrest, J.P., Jackson, R.L., Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (1972) Methods Enzymol, 28, pp. 54-63
  • Shewry, P.R., Plant storage proteins (1995) Bot Rev, 70, pp. 375-426
  • Vitale, A., Bollini, R., Legume storage proteins (1995) Seed Development and Germination, pp. 73-102. , in J Kigel, G Galili, eds. Dekker, New York
  • Werker, F., (1997) Seed Anatomy, , Borntraeger, Berlin

Citas:

---------- APA ----------
Otegui, M., Maldonado, S., Lima, C. & De Lederkremer, R.M. (1999) . Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies. International Journal of Plant Sciences, 160(3), 501-509.
http://dx.doi.org/10.1086/314151
---------- CHICAGO ----------
Otegui, M., Maldonado, S., Lima, C., De Lederkremer, R.M. "Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies" . International Journal of Plant Sciences 160, no. 3 (1999) : 501-509.
http://dx.doi.org/10.1086/314151
---------- MLA ----------
Otegui, M., Maldonado, S., Lima, C., De Lederkremer, R.M. "Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies" . International Journal of Plant Sciences, vol. 160, no. 3, 1999, pp. 501-509.
http://dx.doi.org/10.1086/314151
---------- VANCOUVER ----------
Otegui, M., Maldonado, S., Lima, C., De Lederkremer, R.M. Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies. Int. J. Plant Sci. 1999;160(3):501-509.
http://dx.doi.org/10.1086/314151