Artículo
La Spina, F.A.; Romanato, M.; Brugo-Olmedo, S.; De Vincentiis, S.; Julianelli, V.; Rivera, R.M.; Buffone, M.G. "Heterogeneous distribution of histone methylation in mature human sperm" (2014) Journal of Assisted Reproduction and Genetics. 31(1):45-49
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Abstract:
Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm. Conclusions: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility. © 2013 Springer Science+Business Media.
Registro:
| Documento: | Artículo |
| Título: | Heterogeneous distribution of histone methylation in mature human sperm |
| Autor: | La Spina, F.A.; Romanato, M.; Brugo-Olmedo, S.; De Vincentiis, S.; Julianelli, V.; Rivera, R.M.; Buffone, M.G. |
| Filiación: | Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Centro Médico Seremas, Buenos Aires, Argentina Animal Science Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO, United States |
| Palabras clave: | Epigenetic; Histone modifications; Human sperm; Sperm subpopulations; histone; article; cell differentiation; cell structure; controlled study; ejaculation; HeLa cell; histone acetylation; histone methylation; histone modification; human; human cell; immunocytochemistry; immunofluorescence; male; normal human; nucleosome; priority journal; prospective study; protein processing; semen analysis; spermatid; spermatozoon; spermatozoon maturation; Cell Nucleus; Epigenesis, Genetic; HeLa Cells; Histone-Lysine N-Methyltransferase; Histones; Humans; Male; Methylation; Semen Analysis; Spermatogenesis; Spermatozoa |
| Año: | 2014 |
| Volumen: | 31 |
| Número: | 1 |
| Página de inicio: | 45 |
| Página de fin: | 49 |
| DOI: | http://dx.doi.org/10.1007/s10815-013-0137-4 |
| Título revista: | Journal of Assisted Reproduction and Genetics |
| Título revista abreviado: | J. Assisted Reprod. Genet. |
| ISSN: | 10580468 |
| CODEN: | JARGE |
| CAS: | histone, 9062-68-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v31_n1_p45_LaSpina |
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Citas:
---------- APA ----------
La Spina, F.A., Romanato, M., Brugo-Olmedo, S., De Vincentiis, S., Julianelli, V., Rivera, R.M. & Buffone, M.G. (2014) . Heterogeneous distribution of histone methylation in mature human sperm. Journal of Assisted Reproduction and Genetics, 31(1), 45-49.http://dx.doi.org/10.1007/s10815-013-0137-4
---------- CHICAGO ----------
La Spina, F.A., Romanato, M., Brugo-Olmedo, S., De Vincentiis, S., Julianelli, V., Rivera, R.M., et al. "Heterogeneous distribution of histone methylation in mature human sperm" . Journal of Assisted Reproduction and Genetics 31, no. 1 (2014) : 45-49.http://dx.doi.org/10.1007/s10815-013-0137-4
---------- MLA ----------
La Spina, F.A., Romanato, M., Brugo-Olmedo, S., De Vincentiis, S., Julianelli, V., Rivera, R.M., et al. "Heterogeneous distribution of histone methylation in mature human sperm" . Journal of Assisted Reproduction and Genetics, vol. 31, no. 1, 2014, pp. 45-49.http://dx.doi.org/10.1007/s10815-013-0137-4
---------- VANCOUVER ----------
La Spina, F.A., Romanato, M., Brugo-Olmedo, S., De Vincentiis, S., Julianelli, V., Rivera, R.M., et al. Heterogeneous distribution of histone methylation in mature human sperm. J. Assisted Reprod. Genet. 2014;31(1):45-49.http://dx.doi.org/10.1007/s10815-013-0137-4
