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Abstract:

Summary: Purpose: Serum supplementation has shown to have beneficial effects on in vitro bovine embryo development. However, it is often assumed that serum supplementation may produce mitochondrial damage and this damage would generate lipid accumulation, a major obstacle for cryopreservation. The aim of the present study is to investigate the previous assumptions in early embryonic stages. Methods: We considered in vitro produced bovine embryos from day 1 to 4 of development, which were grown in presence of serum from days 1, 2 or 3 or in absence of it. Electron transmission micrographs allowed us to quantify the area occupied by lipid droplets and by the different mitochondrial types to evaluate serum effect. Using confocal microscopy we analyzed mitochondrial activity and location. Results: We found no evidence of lipid droplets accumulation or mitochondrial degeneration or reduction of mitochondrial area in serum supplemented media. Further, our results suggest that events of mitochondrial proliferation are taking place even in serum supplemented media. Conclusions: Serum does not produce lipid accumulation or mitochondrial damage in bovine embryos from 2 to 16 cells. When serum was added to embryo culture medium on day 3 of development, there were ultrastructural signs of a beneficial effect for embryo development. The lack of serum until day 3 may also avoid the unnecessary exposure to potentially inhibitory factors present on it. © 2013 Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?
Autor:Crocco, M.C.; Kelmansky, D.M.; Mariano, M.I.
Filiación:Instituto Nacional de Parasitología M. F. Chaben ANLIS Malbrán, Av. Paseo Colón 568, Ciudad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Instituto de Cálculo, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Embryo; Lipid droplet; Mitochondria; Serum; Ultrastructure; fat droplet; article; cell adhesion; cell organelle; cell ultrastructure; controlled study; cow; embryo; embryo development; in vitro study; lipid storage; mitochondrial dynamics; mitochondrial volume; mitochondrion; nonhuman; priority journal; serum; Bovinae; Animals; Breeding; Cattle; Cryopreservation; Culture Media, Serum-Free; Embryo Culture Techniques; Embryo, Mammalian; Embryonic Development; Female; Lipids; Mitochondria; Pregnancy; Reproductive Techniques, Assisted; Serum; Zygote
Año:2013
Volumen:30
Número:10
Página de inicio:1377
Página de fin:1388
DOI: http://dx.doi.org/10.1007/s10815-013-0060-8
Título revista:Journal of Assisted Reproduction and Genetics
Título revista abreviado:J. Assisted Reprod. Genet.
ISSN:10580468
CODEN:JARGE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v30_n10_p1377_Crocco

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Citas:

---------- APA ----------
Crocco, M.C., Kelmansky, D.M. & Mariano, M.I. (2013) . Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?. Journal of Assisted Reproduction and Genetics, 30(10), 1377-1388.
http://dx.doi.org/10.1007/s10815-013-0060-8
---------- CHICAGO ----------
Crocco, M.C., Kelmansky, D.M., Mariano, M.I. "Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?" . Journal of Assisted Reproduction and Genetics 30, no. 10 (2013) : 1377-1388.
http://dx.doi.org/10.1007/s10815-013-0060-8
---------- MLA ----------
Crocco, M.C., Kelmansky, D.M., Mariano, M.I. "Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?" . Journal of Assisted Reproduction and Genetics, vol. 30, no. 10, 2013, pp. 1377-1388.
http://dx.doi.org/10.1007/s10815-013-0060-8
---------- VANCOUVER ----------
Crocco, M.C., Kelmansky, D.M., Mariano, M.I. Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?. J. Assisted Reprod. Genet. 2013;30(10):1377-1388.
http://dx.doi.org/10.1007/s10815-013-0060-8