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Abstract:

A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease expression. The protease activity belongs to the class of cysteine proteases since they are inhibited by E64 and Leupeptin and also they are activated by 2-mercaptoethanol and cysteine. They showed good thermal stability. Three active protease bands were found in zymograms of Allium sativum. The in vitro system revealed a significantly higher protease level than storage and embryo tissues of in vivo bulbs.

Registro:

Documento: Artículo
Título:Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
Autor:Parisi, M.; Moreno, S.; Fernández, C.
Filiación:Laboratorio de Química Biológica, Departmento de Ciencias Básicas, Universidad Nacional de Luján, CC: 221, Luján (6700), Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas ‘Fundación Campomar’, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, I.I.B.B.A.-CONICET, Argentina
Palabras clave:Callus culture; Cysteine protease; Endopeptidase; In vitro culture; Protease modulators; Allium sativum; Sativum
Año:2002
Volumen:38
Número:6
Página de inicio:608
Página de fin:612
DOI: http://dx.doi.org/10.1079/IVP2002344
Título revista:In Vitro Cellular and Developmental Biology - Plant
Título revista abreviado:In Vitro Cell. Dev. Biol. Plant
ISSN:10545476
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10545476_v38_n6_p608_Parisi

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Citas:

---------- APA ----------
Parisi, M., Moreno, S. & Fernández, C. (2002) . Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.). In Vitro Cellular and Developmental Biology - Plant, 38(6), 608-612.
http://dx.doi.org/10.1079/IVP2002344
---------- CHICAGO ----------
Parisi, M., Moreno, S., Fernández, C. "Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)" . In Vitro Cellular and Developmental Biology - Plant 38, no. 6 (2002) : 608-612.
http://dx.doi.org/10.1079/IVP2002344
---------- MLA ----------
Parisi, M., Moreno, S., Fernández, C. "Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)" . In Vitro Cellular and Developmental Biology - Plant, vol. 38, no. 6, 2002, pp. 608-612.
http://dx.doi.org/10.1079/IVP2002344
---------- VANCOUVER ----------
Parisi, M., Moreno, S., Fernández, C. Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.). In Vitro Cell. Dev. Biol. Plant. 2002;38(6):608-612.
http://dx.doi.org/10.1079/IVP2002344