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The objective of this study was to isolate strains of the genus Bacillus from different productive soils of the province of Salta, Argentina, which have growth promoting properties in common bean (Phaseolus vulgaris L.) and have the ability to inhibit different phytopathogenic fungi, primarily Macrophomina phaseolina. Among the 105 strains of bacilli checked, Bacillus sp. B14 was selected for having the greatest in vitro inhibitory effect against Sclerotium rolfsii, Sclerotinia sclerotiurum, Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina, recording fungal inhibition values that varied between 60 and 80%. In addition, B14 produced auxins in a concentration of 10.10 mg/ml, and qualitatively synthesizes siderophores. Based on 16S rRNA gene sequencing, the strain was characterized as B. amyloliquefaciens. Data from greenhouse experiments showed that the black common bean cv. Nag 12 seeds inoculated with B14, had increased germination of 10%, as well as an increase in root length of 2 cm and in shoot length of 6 cm compared with the non-inoculated control seeds. When the phytosanitary state of the B14 inoculated seeds was analyzed, no growth of bacteria, or phytopathogenic fungi and contaminants was observed, while in the non-inoculated seeds, bacteria was found in 46% of seeds, in addition to other phytopathogenic fungi. B. amyloliquefaciens B14 reduced the incidence of M. phaseolina by 62% in the inoculated black bean cv. Nag 12 seeds. Furthermore, using MALDI-MS it was determined that the bacteria synthesized different lipopeptides in the presence of M. phaseolina, such as surfactin, iturin, fengycin, kurstatin and polymyxin, leading us to conclude that they are the main responsible for the antagonistic effect observed and that the nature of lipopeptides synthesized by B14 is influenced by target fungal strain. © 2017 Elsevier Inc.


Documento: Artículo
Título:Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
Autor:Sabaté, D.C.; Pérez Brandan, C.; Petroselli, G.; Erra-Balsells, R.; Audisio, M.C.
Filiación:Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Av. Bolivia 5150Salta 4400, Argentina
Instituto Nacional de Tecnología Agropecuaria (INTA)-Estación Experimental Salta, Ruta Nacional 68 Km 172, Cerrillos, Salta 4403, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica Pabellón II, 3er P, Ciudad Universitaria, Buenos Aires, 1428, Argentina
CONICET, Universidad de Buenos Aires, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales Pabellón II, 3er P, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:Bacillus amyloliquefaciens; Lipopeptides; Macrophomina phaseolina; MALDI-MS; PGPR; Phaseolus vulgaris L.
Página de inicio:1
Página de fin:8
Título revista:Biological Control
Título revista abreviado:Biol. Control


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---------- APA ----------
Sabaté, D.C., Pérez Brandan, C., Petroselli, G., Erra-Balsells, R. & Audisio, M.C. (2017) . Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties. Biological Control, 113, 1-8.
---------- CHICAGO ----------
Sabaté, D.C., Pérez Brandan, C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. "Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties" . Biological Control 113 (2017) : 1-8.
---------- MLA ----------
Sabaté, D.C., Pérez Brandan, C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. "Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties" . Biological Control, vol. 113, 2017, pp. 1-8.
---------- VANCOUVER ----------
Sabaté, D.C., Pérez Brandan, C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties. Biol. Control. 2017;113:1-8.