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Abstract:

Problem: Women with celiac disease (CD) are often affected by atypical presentations of the disease associated with reproductive disorders as a main extra-digestive complaint. Here, we analyzed if autoantibodies against tissue transglutaminase (tTG) in sera from CD patients with reproductive disorders could display direct effects through their interaction with tTG expressed on trophoblast cells and phagocytes inducing tissue damage and interfering in the clearance of trophoblast apoptotic bodies. Method of study: Sera from CD women with reproductive disorders were obtained, and their ability to induce apoptosis of Swan-71 (cytotrophoblast cell line) and to modulate the wound-healing and phagocytes process was tested. Results: Swan-71 cells expressed tTG and CD sera displayed a significant decrease in trophoblast cell migration and a delay in injury healing on trophoblast cells, compared with those observed with control sera. Moreover, CD sera significantly reduced trophoblast cell proliferation and increased apoptosis levels in comparison with those observed in the control sera. Finally, autoantibodies against tTG interfere in the clearance of trophoblast apoptotic bodies through a mechanism involving MFG-E8 (milk fat globulin-EGF factor 8)-tTG binding. Conclusion: The anti-tTG antibodies might contribute to trophoblast damage and disrupt the phagocytosis process of apoptotic bodies that could promote a pro-inflammatory microenvironment. © 2014 John Wiley & Sons A/S.

Registro:

Documento: Artículo
Título:Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients
Autor:Sóñora, C.; Calo, G.; Fraccaroli, L.; Pérez-Leirós, C.; Hernández, A.; Ramhorst, R.
Filiación:Immunology Laboratory, School of Sciences/School of Chemistry, Montevideo, Uruguay
EUTM-School of Medicine UDELAR, Montevideo, Uruguay
Immunopharmacology Laboratory, School of Sciences, University of Buenos Aires and National Research Council (IQUIBICEN-CONICET), Buenos Aires, Argentina
Palabras clave:Celiac patients; Pregnancy complications; Tissue transglutaminase; lactadherin; protein glutamine gamma glutamyltransferase; protein glutamine gamma glutamyltransferase antibody; autoantibody; guanine nucleotide binding protein; protein glutamine gamma glutamyltransferase; protein glutamine gamma glutamyltransferase 2; adult; antibody blood level; apoptosis; Article; celiac disease; cell migration; cell proliferation; clearance; clinical article; controlled study; cytotrophoblast; female; human; human cell; human tissue; phagocyte; pregnancy complication; priority journal; protein binding; protein expression; protein interaction; tissue injury; trophoblast; Western blotting; wound healing; blood; celiac disease; cell line; enzymology; immunology; metabolism; middle aged; pregnancy; pregnancy complication; trophoblast; Adult; Autoantibodies; Celiac Disease; Cell Line; Female; GTP-Binding Proteins; Humans; Middle Aged; Pregnancy; Pregnancy Complications; Transglutaminases; Trophoblasts
Año:2014
Volumen:72
Número:5
Página de inicio:485
Página de fin:495
DOI: http://dx.doi.org/10.1111/aji.12290
Título revista:American Journal of Reproductive Immunology
Título revista abreviado:Am. J. Reprod. Immunol.
ISSN:10467408
CODEN:AAJID
CAS:protein glutamine gamma glutamyltransferase, 80146-85-6; Autoantibodies; GTP-Binding Proteins; transglutaminase 2; Transglutaminases
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v72_n5_p485_Sonora

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Citas:

---------- APA ----------
Sóñora, C., Calo, G., Fraccaroli, L., Pérez-Leirós, C., Hernández, A. & Ramhorst, R. (2014) . Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients. American Journal of Reproductive Immunology, 72(5), 485-495.
http://dx.doi.org/10.1111/aji.12290
---------- CHICAGO ----------
Sóñora, C., Calo, G., Fraccaroli, L., Pérez-Leirós, C., Hernández, A., Ramhorst, R. "Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients" . American Journal of Reproductive Immunology 72, no. 5 (2014) : 485-495.
http://dx.doi.org/10.1111/aji.12290
---------- MLA ----------
Sóñora, C., Calo, G., Fraccaroli, L., Pérez-Leirós, C., Hernández, A., Ramhorst, R. "Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients" . American Journal of Reproductive Immunology, vol. 72, no. 5, 2014, pp. 485-495.
http://dx.doi.org/10.1111/aji.12290
---------- VANCOUVER ----------
Sóñora, C., Calo, G., Fraccaroli, L., Pérez-Leirós, C., Hernández, A., Ramhorst, R. Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients. Am. J. Reprod. Immunol. 2014;72(5):485-495.
http://dx.doi.org/10.1111/aji.12290