Artículo
Fontana, V.A.; Sanchez, M.; Cebral, E.; Calvo, J.C. "Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration" (2010) American Journal of Reproductive Immunology. 64(1):20-26
Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor
Abstract:
Problem Establishment of a successful pregnancy relies on a complex fetal-mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in implantation and could affect the success of pregnancy.Method of study Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-γ and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.Results Interferon-γ inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.Conclusion Our results correlate with previous reports from our laboratory indicating that IFN- γ is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion. © 2010 John Wiley & Sons A/S.
Registro:
| Documento: | Artículo |
| Título: | Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration |
| Autor: | Fontana, V.A.; Sanchez, M.; Cebral, E.; Calvo, J.C. |
| Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Laboratorio de Biología del Desarrollo, Instituto de Fisiología, Biología Molecular y Neurociencias, DBBE,Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina |
| Palabras clave: | Cytokines; Extracellular matrix; Placenta; gamma interferon; gelatinase; gelatinase A; gelatinase B; leptin; metalloproteinase; gamma interferon; gelatinase A; gelatinase B; leptin; article; cell culture; cell migration; cell strain JEG 3; controlled study; cytotrophoblast; enzyme activity; female; human; human cell; human cell culture; pregnancy; priority journal; protein synthesis; Western blotting; zymography; animal; biosynthesis; cell motion; drug effect; enzymology; metabolism; mouse; physiology; polyacrylamide gel electrophoresis; trophoblast; tumor cell line; Animals; Blotting, Western; Cell Line, Tumor; Cell Movement; Electrophoresis, Polyacrylamide Gel; Female; Humans; Interferon-gamma; Leptin; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Mice; Trophoblasts |
| Año: | 2010 |
| Volumen: | 64 |
| Número: | 1 |
| Página de inicio: | 20 |
| Página de fin: | 26 |
| DOI: | http://dx.doi.org/10.1111/j.1600-0897.2010.00816.x |
| Título revista: | American Journal of Reproductive Immunology |
| Título revista abreviado: | Am. J. Reprod. Immunol. |
| ISSN: | 10467408 |
| CODEN: | AAJID |
| CAS: | gamma interferon, 82115-62-6; gelatinase, 9040-48-6; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; metalloproteinase, 81669-70-7; Interferon-gamma, 82115-62-6; Leptin; Matrix Metalloproteinase 2, 3.4.24.24; Matrix Metalloproteinase 9, 3.4.24.35 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v64_n1_p20_Fontana |
Referencias:
- Das, R.M., Martin, L., Uterine DNA synthesis and cell proliferation during early decidualization induced by oil in mice (1978) J Reprod Fertil, 53, pp. 125-128
- Lejeune, B., Van Hoeck, J., Leroy, F., Satellite versus total DNA replication in relation to endopolyploidy of decidual cells in the mouse (1982) Chromosoma, 84, pp. 511-516
- Stewart, I., Peel, S., The differentiation of the decidua and the distribution of metrial gland cells in the pregnant mouse uterus (1978) Cell Tissue Res, 187, pp. 167-179
- Mukhtar, D.D., Stewart, I.J., Croy, B.A., Leucocyte membrane antigens on mouse granulated metrial gland cells (1989) J Reprod Immunol, 15, pp. 269-279
- Linnemeyer, P.A., Pollack, S.B., Murine granulated metrial gland cells at uterine implantation sites are natural killer lineage cells (1991) J Immunol, 147, pp. 2530-2535
- Parr, E.L., Parr, M.B., Zheng, L.M., Young, J., Mouse granulated metrial gland cells originate by local activation of uterine natural killer lymphocytes (1991) Biol Reprod, 44, pp. 834-841
- Ashkar, A.A., Di Santo, J.P., Croy, B.A., Interferon- contributes to initiation of uterine vascular modification, decidual integrity, and uterine natural killer cell maturation during normal murine pregnancy (2000) J Exp Med, 192, pp. 259-270
- Chantakru, S., Miller, C., Roach, L.E., Kuziel, W.A., Maeda, N., Wang, W.C., Evans, S.S., Croy, B.A., Contributions from self-renewal and trafficking to the uterine NK cell population of early pregnancy (2002) J Immunol, 168, pp. 22-28
- Billington, W.D., The immunological problem of pregnancy: 50 years with the hope of progress. A tribute to Peter Medawar (2003) J Reprod Immunol, 60, pp. 1-11
- Cameo, M., Fontana, V., Cameo, P., Vauthay, L.G., Kaplan, J., Tesone, M., Similar embryotoxic effects of sera from infertile patients and exogenous interferon-γ on long-term in-vitro development of mouse embryos (1999) Hum Reprod, 14, pp. 959-963
- Fontana, V., Choren, V., Vauthay, L., Calvo, J.C., Calvo, L., Cameo, M., Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) (2004) Reproduction, 128, pp. 717-725
Citas:
---------- APA ----------
Fontana, V.A., Sanchez, M., Cebral, E. & Calvo, J.C. (2010) . Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration. American Journal of Reproductive Immunology, 64(1), 20-26.http://dx.doi.org/10.1111/j.1600-0897.2010.00816.x
---------- CHICAGO ----------
Fontana, V.A., Sanchez, M., Cebral, E., Calvo, J.C. "Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration" . American Journal of Reproductive Immunology 64, no. 1 (2010) : 20-26.http://dx.doi.org/10.1111/j.1600-0897.2010.00816.x
---------- MLA ----------
Fontana, V.A., Sanchez, M., Cebral, E., Calvo, J.C. "Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration" . American Journal of Reproductive Immunology, vol. 64, no. 1, 2010, pp. 20-26.http://dx.doi.org/10.1111/j.1600-0897.2010.00816.x
---------- VANCOUVER ----------
Fontana, V.A., Sanchez, M., Cebral, E., Calvo, J.C. Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration. Am. J. Reprod. Immunol. 2010;64(1):20-26.http://dx.doi.org/10.1111/j.1600-0897.2010.00816.x
