Cristina, C.; Perez-Millan, M.I.; Luque, G.; Dulce, R.A.; Sevlever, G.; Berner, S.I.; Becu-Villalobos, D. "VEGF and CD31 association in pituitary adenomas" (2010) Endocrine Pathology. 21(3):154-160
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Pituitary tumors are usually less vascularized than the normal pituitary, and the role of angiogenesis in these adenomas is contentious. Appraisal of microvascular density and expression of the potent angiogenic vascular endothelial growth factor (VEGF) by immunohistochemistry has yielded controversial results, as a broad spectrum of immunostaining can be found. We determined the protein expression of VEGF and CD31, an endothelial marker, in a series of 56 surgically removed pituitary adenomas using Western blot assay. Prolactinomas had higher VEGF protein expression compared to nonfunctioning or ACTH- and GH-secreting adenomas, while CD31 was similar in the different adenoma histotypes. VEGF and CD31 were not affected by sex, age, years of adenoma evolution, or proliferation rate (Ki67 and PCNA) for all adenoma types. Only in nonfunctioning adenomas CD31 concentration increased significantly with age. There was a positive correlation between CD31 and VEGF expression when all adenoma histotypes were considered, or when prolactinomas and nonfunctioning adenomas were evaluated separately. The positive association of VEGF and CD31 expression suggests the participation of angiogenesis in adenoma development, while epithelial cell proliferation in pituitary tumors is not directly related to VEGF or CD31 expression, and other factors, such as primary genetic alterations may be involved. © 2010 Springer Science+Business Media, LLC.


Documento: Artículo
Título:VEGF and CD31 association in pituitary adenomas
Autor:Cristina, C.; Perez-Millan, M.I.; Luque, G.; Dulce, R.A.; Sevlever, G.; Berner, S.I.; Becu-Villalobos, D.
Filiación:Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Universidad Nacional Del Noroeste de la Provincia de Buenos Aires (UNNOBA), Roque Sáenz Peña 456, 6000 Buenos Aires Junín, Argentina
Department of Neuropathology, Institute for Neurological Research FLENI, Montañeses 2325, Buenos Aires C1428AQK Buenos Aires, Argentina
Department of Neurosurgery, Hospital Santa Lucía, Buenos Aires 1232, Argentina
Palabras clave:Angiogenesis; CD31; Pelioisis; Pituitary adenoma; Proliferation; VEGF; CD31 antigen; cycline; Ki 67 antigen; vasculotropin; adult; age; angiogenesis; article; carcinogenesis; cell proliferation; correlation analysis; disease course; epithelium cell; female; gender; growth hormone secreting adenoma; histopathology; human; human tissue; hypophysis adenoma; major clinical study; male; mutation; priority journal; prolactinoma; protein expression; tumor growth; Western blotting; Adenoma; Adult; Antigens, CD31; Blotting, Western; Female; Humans; Male; Pituitary Neoplasms; Tumor Markers, Biological; Vascular Endothelial Growth Factor A
Página de inicio:154
Página de fin:160
Título revista:Endocrine Pathology
Título revista abreviado:Endocr. Pathol.
CAS:vasculotropin, 127464-60-2; Antigens, CD31; Tumor Markers, Biological; Vascular Endothelial Growth Factor A


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---------- APA ----------
Cristina, C., Perez-Millan, M.I., Luque, G., Dulce, R.A., Sevlever, G., Berner, S.I. & Becu-Villalobos, D. (2010) . VEGF and CD31 association in pituitary adenomas. Endocrine Pathology, 21(3), 154-160.
---------- CHICAGO ----------
Cristina, C., Perez-Millan, M.I., Luque, G., Dulce, R.A., Sevlever, G., Berner, S.I., et al. "VEGF and CD31 association in pituitary adenomas" . Endocrine Pathology 21, no. 3 (2010) : 154-160.
---------- MLA ----------
Cristina, C., Perez-Millan, M.I., Luque, G., Dulce, R.A., Sevlever, G., Berner, S.I., et al. "VEGF and CD31 association in pituitary adenomas" . Endocrine Pathology, vol. 21, no. 3, 2010, pp. 154-160.
---------- VANCOUVER ----------
Cristina, C., Perez-Millan, M.I., Luque, G., Dulce, R.A., Sevlever, G., Berner, S.I., et al. VEGF and CD31 association in pituitary adenomas. Endocr. Pathol. 2010;21(3):154-160.