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Casabuono, A.C.; Czibener, C.; Del Giudice, M.G.; Valguarnera, E.; Ugalde, J.E.; Couto, A.S. "New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide" (2017) Journal of the American Society for Mass Spectrometry. 28(12):2716-2723
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Abstract:

Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.]. © 2017, American Society for Mass Spectrometry.

Registro:

Documento: Artículo
Título:New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide
Autor:Casabuono, A.C.; Czibener, C.; Del Giudice, M.G.; Valguarnera, E.; Ugalde, J.E.; Couto, A.S.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica - Consejo Nacional de Investigaciones Científicas y Técnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Ciudad Universitaria, Intendente Güiraldes 2160, C1428GA, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde”, IIB-INTECH, CONICET, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina
Palabras clave:Brucella; Lipid A; Lipopolysaccharide; MALDI-TOF MS; Acylation; Cell membranes; Mass spectrometry; Negative ions; Brucella; Core oligosaccharide; Gram-negative bacteria; Lipid A; Lipopolysaccharides; MALDI TOF MS; MALDI-TOF mass spectrometry; Structural studies; Bacteria; bacterium lipopolysaccharide; disaccharide; lipid A; phosphate; phosphoethanolamine; pyrophosphorylethanolamine; unclassified drug; acylation; Article; Brucella abortus; Brucella suis; lipid analysis; matrix assisted laser desorption ionization time of flight mass spectrometry; negative ion electrospray; nonhuman; positive ion electrospray; tandem mass spectrometry
Año:2017
Volumen:28
Número:12
Página de inicio:2716
Página de fin:2723
DOI: http://dx.doi.org/10.1007/s13361-017-1805-x
Título revista:Journal of the American Society for Mass Spectrometry
Título revista abreviado:J. Am. Soc. Mass Spectrom.
ISSN:10440305
CODEN:JAMSE
CAS:lipid A, 95991-05-2; phosphate, 14066-19-4, 14265-44-2; phosphoethanolamine, 1071-23-4, 29868-05-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v28_n12_p2716_Casabuono

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Citas:

---------- APA ----------
Casabuono, A.C., Czibener, C., Del Giudice, M.G., Valguarnera, E., Ugalde, J.E. & Couto, A.S. (2017) . New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide. Journal of the American Society for Mass Spectrometry, 28(12), 2716-2723.
http://dx.doi.org/10.1007/s13361-017-1805-x
---------- CHICAGO ----------
Casabuono, A.C., Czibener, C., Del Giudice, M.G., Valguarnera, E., Ugalde, J.E., Couto, A.S. "New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide" . Journal of the American Society for Mass Spectrometry 28, no. 12 (2017) : 2716-2723.
http://dx.doi.org/10.1007/s13361-017-1805-x
---------- MLA ----------
Casabuono, A.C., Czibener, C., Del Giudice, M.G., Valguarnera, E., Ugalde, J.E., Couto, A.S. "New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide" . Journal of the American Society for Mass Spectrometry, vol. 28, no. 12, 2017, pp. 2716-2723.
http://dx.doi.org/10.1007/s13361-017-1805-x
---------- VANCOUVER ----------
Casabuono, A.C., Czibener, C., Del Giudice, M.G., Valguarnera, E., Ugalde, J.E., Couto, A.S. New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide. J. Am. Soc. Mass Spectrom. 2017;28(12):2716-2723.
http://dx.doi.org/10.1007/s13361-017-1805-x