Navegar

Colección

Datos Estadísticas

Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009.

Registro:

Documento: Artículo
Título:Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
Autor:Yu, Z.; Chen, L.C.; Suzuki, H.; Ariyada, O.; Erra-Balsells, R.; Nonami, H.; Hiraoka, K.
Filiación:Clean Energy Research Center, University of Yamanashi, Kofu, Japan
ARIOS Inc., Akishima, Tokyo, Japan
CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan
College of Chemistry and Biology, Shenyang Normal University, Shenyang, China
Palabras clave:Bioactive compounds; Carbohydrate content; Direct analysis; Direct profiling; Electrospray ionization mass spectrometry; Electrosprays; Flower buds; In-vivo; Metabolic changes; Phytochemicals; Sample consumption; Sample preparation; Time-of-flight mass spectrometers; Amines; Amino acids; Biological systems; Carbohydrates; Ionization; Mass spectrometers; Mass spectrometry; Organic acids; Particle detectors; Probes; Vegetation; Electrospray ionization; amino acid; anthocyanin; carbohydrate; pelargonidin; plant medicinal product; amino acid analysis; article; carbohydrate analysis; controlled study; drug structure; electrospray mass spectrometry; flower; foliage; in vivo study; nonhuman; petal; plant bulb; plant growth; plant leaf; plant tissue; time of flight mass spectrometry; tulip; Carbohydrates; Plant Roots; Spectrometry, Mass, Electrospray Ionization; Transducers; Tulipa; Tulipa
Año:2009
Volumen:20
Número:12
Página de inicio:2304
Página de fin:2311
DOI: http://dx.doi.org/10.1016/j.jasms.2009.08.023
Título revista:Journal of the American Society for Mass Spectrometry
Título revista abreviado:J. Am. Soc. Mass Spectrom.
ISSN:10440305
CODEN:JAMSE
CAS:amino acid, 65072-01-7; pelargonidin, 134-04-3; Carbohydrates
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10440305_v20_n12_p2304_Yu.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v20_n12_p2304_Yu

Referencias:

  • Cowan, M.M., Plant Products as Antimicrobial Agents (1999) Clin. Microbiol. Rev., 12, pp. 564-582
  • Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F., Whitehouse, C.M., Electrospray Ionization for Mass Spectrometry of Large Biomolecules (1989) Science, 246, pp. 64-71
  • Fuerstenau, S.D., Benner, W.H., Thomas, J.J., Brugidou, C., Bothner, B., Siuzdak, G., Mass Spectrometry of an Intact Virus (2001) Angew. Chem. Int. Ed., 40, pp. 541-544
  • Takats, Z., Wiseman, J.M., Gologan, B., Cooks, R.G., Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization (2004) Science, 306, pp. 471-473
  • Volny, M., Venter, A., Smith, S.A., Pazzi, M., Cooks, R.G., Surface Effects and Electrochemical Cell Capacitance in Desorption Electrospray Ionization (2008) Analyst, 133, pp. 525-531
  • Cooks, R.G., Ouyang, Z., Takats, Z., Wiseman, J.M., Ambient Mass Spectrometry (2006) Science, 311, pp. 1566-1570
  • Ifa, D.R., Wiseman, J.M., Song, Q., Cooks, R.G., Development of Capabilities for Imaging Mass Spectrometry Under Ambient Conditions with Desorption Electrospray Ionization (DESI) (2007) Int. J. Mass Spectrom., 259, pp. 8-15
  • Haddad, R., Sparrapan, R., Eberlin, M.N., Desorption Sonic Spray Ionization for (High) Voltage-Free Ambient Mass Spectrometry (2006) Rapid Commun. Mass Spectrom., 20, pp. 2901-2905
  • Haddad, R., Sparrapan, R., Kotiaho, T., Eberlin, M.N., Easy Ambient Sonic-Spray Ionization-Membrane Interface Mass Spectrometry for Direct Analysis of Solution Constituents (2008) Anal. Chem., 80, pp. 898-903
  • Huang, M.Z., Hsu, J., Wu, C.I., Lin, S.Y., Ma, Y.L., Cheng, T.L., Shiea, J., Characterization of the Chemical Components on the Surface of Different Solids with Electrospray-Assisted Laser Desorption Ionization Mass Spectrometry (2007) Rapid Commun. Mass Spectrom., 21, pp. 1767-1775
  • Sampson, J.S., Hawkridge, A.M., Muddiman, D.C., Generation and Detection of Multiply-Charged Peptides and Proteins by Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI) Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (2006) J. Am. Soc. Mass Spectrom., 17, pp. 1712-1716
  • Nemes, P., Vertes, A., Laser Ablation Electrospray Ionization for Atmospheric Pressure, In Vivo, and Imaging Mass Spectrometry (2007) Anal. Chem., 79, pp. 8098-8106
  • Hiraoka, K., Nishidate, K., Mori, K., Asakawa, D., Suzuki, S., Development of Probe Electrospray Using a Solid Needle (2007) Rapid Commun. Mass Spectrom., 21, pp. 3139-3144
  • Chen, L.C., Nishidate, K., Saito, Y., Mori, K., Asakawa, D., Takeda, S., Kubota, T., Hiraoka, K., Application of Probe Electrospray to Direct Ambient Analysis of Biological Samples (2008) Rapid Commun. Mass Spectrom., 22, pp. 2366-2374
  • Chen, L.C., Nishidate, K., Saito, Y., Mori, K., Asakawa, D., Takeda, S., Kubota, T., Hiraoka, K., Characteristics of Probe Electrospray Generated from a Solid Needle (2008) J. Phys. Chem. B, 112, pp. 11164-11170
  • Torskangerpoll, K., Nørbæk, R., Nodland, E., Øvstedal, D.O., Andersen Ø., M., Anthocyanin content of Tulipa Species and Cultivars and Its Impact on Tepal Colours (2005) Biochem. Syst. Ecol., 33, pp. 499-510
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., In Situ Analysis of Plant Tissue Underivatized Carbohydrates and On-Probe Enzymatic Degraded Starch by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry by Using Carbon Nanotubes as Matrix (2008) Anal. Biochem., 383, pp. 159-167
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract (2008) J. Am. Soc. Mass Spectrom., 19, pp. 1841-1848
  • Zacharius, R.M., Cathey, H.M., Steward, F.C., Nitrogenous Compounds and Nitrogen Metabolism in the Liliaceae: III. Changes in the Soluble Nitrogen Compounds of the Tulip and Their Relation to Flower Formation in the Bulb (1957) Ann. Bot., 21, pp. 193-201
  • McKee, H.S., (1962) Nitrogen Metabolism in Plants, p. 417. , Clarendon Press, Oxford
  • Holton, T.A., Cornish, E.C., Genetics and Biochemistry of Anthocyanin Biosynthesis (1995) Plant Cell, 7, pp. 1071-1083
  • van Baarlen, P., Legendre, L., van Kan, J., Plant Defence Compounds Against Botrytis Infection (2004) Botrytis: Biology, Pathology and Control, pp. 143-161. , Elad Y., Williamson B., Tudzynski P., and Delen N. (Eds), Kluwer Academic Press, Dordrecht
  • Walch, K., van Hasselt, P.R., The Influence of Low Temperature on the Membrane Lipid Composition and Flowering Capacity of Tulip Bulbs (1991) Acta Hort., 298, pp. 345-354
  • van Kilsdonk, M.G., Nicolay, K., Franssen, J.M., Kolloffel, C., Bud Abortion in Tulip Bulbs Studied by Magnetic Resonance Imaging (2002) J. Exp. Bot., 53, pp. 1603-1611
  • van Rossum, M.W.P.C., Alberda, M., van der Plas, L.H.W., Tulipaline and Tuliposide in Cultured Explants of Tulip Bulb Scales (1998) Phytochemistry, 49, pp. 723-729
  • Kamenetsky, R., Zemah, H., Ranwala, A.P., Vergeldt, F., Ranwala, N.K., Miller, W.B., Van As, H., Bendel, P., Water Status and Carbohydrate Pools in Tulip Bulbs During Dormancy Release (2003) New Phytol., 158, pp. 109-118
  • Iwaya-Inoue, M., Motooka, K., Ishida, N., Koizumi, M., Kano, H., Chilling Effects for Normal Growth of Tulip Bulbs Estimated by MRI (1996) Acta Hort., 440, pp. 407-412
  • Naor, V., Kigel, J., Ben-Tal, Y., Ziv, M., Variation in Endogenous Gibberellins, Abscissic Acid, and Carbohydrate Content During the Growth Cycle of Colored Zantedeschia spp., a Tuberous Geophyte (2008) J. Plant Growth Regul., 27, pp. 211-220
  • Bergman, B., Presence of a Substance in the White Skin of Young Tulip Bulbs That Inhibits Growth of Fusarium oxysporum (1966) Eur. J. Plant Pathol., 72, pp. 222-230
  • Geng, X., Sato, A., Okubo, H., Saniewski, M., Changes in Carbohydrate and ABA Content in Tulip Bulbs During Storage (2007) J. Fac. Agr. Kyushu Univ., 52, pp. 315-319
  • Ranwala, A., Miller, W., Gibberellin-Mediated Changes in Carbohydrate Metabolism During Flower Sstalk Elongation in Tulips (2008) Plant Growth Regul., 55, pp. 241-248
  • Gilford, J., Rees, A., Growth of the Tulip Shoot (1973) Sci. Hort., 1, pp. 143-156

Citas:

---------- APA ----------
Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H. & Hiraoka, K. (2009) . Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 20(12), 2304-2311.
http://dx.doi.org/10.1016/j.jasms.2009.08.023
---------- CHICAGO ----------
Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., et al. "Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry" . Journal of the American Society for Mass Spectrometry 20, no. 12 (2009) : 2304-2311.
http://dx.doi.org/10.1016/j.jasms.2009.08.023
---------- MLA ----------
Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., et al. "Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry" . Journal of the American Society for Mass Spectrometry, vol. 20, no. 12, 2009, pp. 2304-2311.
http://dx.doi.org/10.1016/j.jasms.2009.08.023
---------- VANCOUVER ----------
Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., et al. Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2009;20(12):2304-2311.
http://dx.doi.org/10.1016/j.jasms.2009.08.023