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Abstract:

Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. © 2008 American Society for Mass Spectrometry.

Registro:

Documento: Artículo
Título:UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
Autor:Landoni, M.; Duschak, V.G.; Erra-Balsells, R.; Couto, A.S.
Filiación:CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Investigación, Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, ANLIS-Malbrán, Buenos Aires, Argentina
Palabras clave:Amides; Computer networks; Desorption; Lipids; Mass spectrometers; Mass spectrometry; Matrix algebra; Microfluidics; Network protocols; Pulsed laser deposition; Spectrometers; Spectrometry; Spectrum analysis; American society; Analyte ions; Ceramide; Epimastigote; Glycosphingolipids (GSL); High quality (HQ); MALDI-MS; Mass spectrometry analysis; Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry; Matrix-assisted laser-desorption/ionization (MALDI); Molecular processes; new applications; Sphingolipids; Trypanosoma cruzi; Ultra-violet (UV); High performance liquid chromatography; acetylceramide; ceramide; ceramide derivative; fluorescent dye; galactosylceramide; galactosyldihydroceramide; glucosylceramide; glycosphingolipid; ion; nitrobenz 2 oxa 1,3 diazole; pyrrole derivative; article; calibration; covalent bond; epimastigote; instrumentation; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; signal detection; Trypanosoma cruzi; Animals; Glycosphingolipids; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Trypanosoma cruzi; Sphingoidea; Trypanosoma cruzi
Año:2008
Volumen:19
Número:7
Página de inicio:923
Página de fin:926
DOI: http://dx.doi.org/10.1016/j.jasms.2008.04.017
Título revista:Journal of the American Society for Mass Spectrometry
Título revista abreviado:J. Am. Soc. Mass Spectrom.
ISSN:10440305
CODEN:JAMSE
CAS:galactosylceramide, 85305-88-0; Glycosphingolipids
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10440305_v19_n7_p923_Landoni.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v19_n7_p923_Landoni

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Citas:

---------- APA ----------
Landoni, M., Duschak, V.G., Erra-Balsells, R. & Couto, A.S. (2008) . UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix. Journal of the American Society for Mass Spectrometry, 19(7), 923-926.
http://dx.doi.org/10.1016/j.jasms.2008.04.017
---------- CHICAGO ----------
Landoni, M., Duschak, V.G., Erra-Balsells, R., Couto, A.S. "UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix" . Journal of the American Society for Mass Spectrometry 19, no. 7 (2008) : 923-926.
http://dx.doi.org/10.1016/j.jasms.2008.04.017
---------- MLA ----------
Landoni, M., Duschak, V.G., Erra-Balsells, R., Couto, A.S. "UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix" . Journal of the American Society for Mass Spectrometry, vol. 19, no. 7, 2008, pp. 923-926.
http://dx.doi.org/10.1016/j.jasms.2008.04.017
---------- VANCOUVER ----------
Landoni, M., Duschak, V.G., Erra-Balsells, R., Couto, A.S. UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix. J. Am. Soc. Mass Spectrom. 2008;19(7):923-926.
http://dx.doi.org/10.1016/j.jasms.2008.04.017