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Abstract:

Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry.

Registro:

Documento: Artículo
Título:Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract
Autor:Gholipour, Y.; Nonami, H.; Erra-Balsells, R.
Filiación:The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan
CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:Carbohydrates; Carbon nanotubes; Cells; Cytology; Glucose; Military engineering; Organic compounds; Polysaccharides; Positive ions; Potential flow; Sugar (sucrose); Sugars; Carbohydrate contents; Cell cytoplasms; Dihydroxybenzoic acids; Direct analyses; High resolutions; Leaf cells; Microcapillary tips; Positive ion modes; Pressure probes; Reproducibility; Trihydroxyacetophenone; Biomolecules; 2,4,6 trihydroxyacetophenone; acetophenone derivative; carbon nanotube; cell extract; disaccharide; gentisic acid; hexose; unclassified drug; article; carbohydrate analysis; cytoplasm; matrix assisted laser desorption ionization time of flight mass spectrometry; plant cell; plant leaf; Carbohydrates; Cytoplasm; Plants; Pressure; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tulipa; Ultraviolet Rays; Tulipa
Año:2008
Volumen:19
Número:12
Página de inicio:1841
Página de fin:1848
DOI: http://dx.doi.org/10.1016/j.jasms.2008.08.006
Título revista:Journal of the American Society for Mass Spectrometry
Título revista abreviado:J. Am. Soc. Mass Spectrom.
ISSN:10440305
CODEN:JAMSE
CAS:gentisic acid, 490-79-9; hexose, 93780-23-5; Carbohydrates
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10440305_v19_n12_p1841_Gholipour.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v19_n12_p1841_Gholipour

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Citas:

---------- APA ----------
Gholipour, Y., Nonami, H. & Erra-Balsells, R. (2008) . Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract. Journal of the American Society for Mass Spectrometry, 19(12), 1841-1848.
http://dx.doi.org/10.1016/j.jasms.2008.08.006
---------- CHICAGO ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. "Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract" . Journal of the American Society for Mass Spectrometry 19, no. 12 (2008) : 1841-1848.
http://dx.doi.org/10.1016/j.jasms.2008.08.006
---------- MLA ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. "Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract" . Journal of the American Society for Mass Spectrometry, vol. 19, no. 12, 2008, pp. 1841-1848.
http://dx.doi.org/10.1016/j.jasms.2008.08.006
---------- VANCOUVER ----------
Gholipour, Y., Nonami, H., Erra-Balsells, R. Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract. J. Am. Soc. Mass Spectrom. 2008;19(12):1841-1848.
http://dx.doi.org/10.1016/j.jasms.2008.08.006