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We screened for pollen-specific kinase genes, which are potential signal transduction components of pollen-pistil interactions, and isolated two structurally related receptor-like kinases (RLKs) from tomato, LePRK1 and LePRK2. These kinases are similar to a pollen-expressed RLK from petunia, but they are expressed later during pollen development than is the petunia RLK. The abundance of LePRK2 increases when pollen germinates, but LePRK1 remains constant. Both LePRK1 and LePRK2 are localized to the plasma membrane/cell wall of growing pollen tubes. Both kinase domains have kinase activity when expressed in Escherichia coli. In phosphorylation assays with pollen membrane preparations, LePRK2, but not LePRK1, is phosphorylated, and the addition of tomato style, but not leaf, extracts to these membrane preparations results at least partially in specific dephosphorylation of LePRK2. Taken together, these results suggest that LePRK1 and LePRK2 play different roles in postpollination events and that at least LePRK2 may mediate some pistil response.


Documento: Artículo
Título:Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2
Autor:Muschietti, J.; Eyal, Y.; McCormick, S.
Filiación:Plant Gene Expression Center, U.S. Department of Agriculture, University of California at Berkeley, 800 Buchanan Street, Albany, CA 94710, United States
Inst. de Ing. Genet./Biol. Molecular, University of Buenos Aires, Vuelta de Obligado 2490, Buenos Aires (1428), Argentina
Institute of Horticulture, Volcani Center, ARO, P.O. Box 6, Bet-Dagan 50250, Israel
Plant Gene Expression Center, USDA-ARS, 800 Buchanan St., Albany, CA 94710, United States
Palabras clave:membrane protein; protein kinase; recombinant protein; amino acid sequence; article; cell wall; enzymology; Escherichia coli; gene; gene expression; genetics; germination; in vitro study; metabolism; molecular genetics; nucleotide sequence; phosphorylation; physiology; plant; pollen; sequence alignment; tomato; Amino Acid Sequence; Cell Wall; Escherichia coli; Gene Expression; Genes, Plant; Germination; Lycopersicon esculentum; Membrane Proteins; Molecular Sequence Data; Phosphorylation; Plant Components; Pollen; Protein Kinases; Recombinant Proteins; Sequence Alignment; Escherichia coli; Lycopersicon esculentum; Petunia
Página de inicio:319
Página de fin:330
Título revista:Plant Cell
Título revista abreviado:Plant Cell
CAS:Membrane Proteins; Protein Kinases, EC; Recombinant Proteins


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---------- APA ----------
Muschietti, J., Eyal, Y. & McCormick, S. (1998) . Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2. Plant Cell, 10(3), 319-330.
---------- CHICAGO ----------
Muschietti, J., Eyal, Y., McCormick, S. "Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2" . Plant Cell 10, no. 3 (1998) : 319-330.
---------- MLA ----------
Muschietti, J., Eyal, Y., McCormick, S. "Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2" . Plant Cell, vol. 10, no. 3, 1998, pp. 319-330.
---------- VANCOUVER ----------
Muschietti, J., Eyal, Y., McCormick, S. Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2. Plant Cell. 1998;10(3):319-330.