Artículo

Coll, T.A.; Chaufan, G.; Pérez-Tito, L.; Ventureira, M.R.; Sobarzo, C.M.A.; Ríos de Molina, M.D.C.; Cebral, E. "Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake" (2017) Molecular Reproduction and Development. 84(10):1086-1099
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Abstract:

Perigestational alcohol consumption by CF-1 mouse, from before mating up to the period of embryo organogenesis, leads to retarded early embryo development and neural tube defects. Here, we addressed if perigestational alcohol ingestion up to Day 10 of pregnancy induces oxidative stress and changes in macromolecules and organ tissues of early organogenic embryos. Adult CF-1 female mice were administered 10% ethanol in their drinking water for 17 days prior to mating and until Day 10 of gestation, whereas control females were administered ethanol-free water. Our results demonstrated significantly reduced Catalase abundance and activity and increased glutathione content in the embryos of ethanol-treated females. The nitrite level was significantly reduced, but TBARS (thiobarbituric acid reactive substances) content, an index of lipid peroxidation, did not change. Embryos derived from ethanol-treated females also showed higher abundance of 3-nitrotyrosine (3-NT)-containing proteins in all tissues, compared to the control group. Apoptosis was significantly increased in the ectoderm and mesoderm, but not in the heart—although this organ did contain more cleaved Caspase-3-positive cardiomyocytes per area of ventricular myocardium than controls. In sum, moderate perigestational alcohol ingestion up to Day 10 of gestation in mice induces oxidative stress by altering radical nitrogen species and antioxidant enzymatic and non-enzymatic mechanisms in embryos. Further, generalized protein nitration, due to unbalanced nitric oxide levels associated with tissue-specific apoptosis, was detected in embryos, suggesting that oxidative mechanisms may play an important role in the perigestational alcohol-induced malformation of organogenic embryos exposed to ethanol. © 2017 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake
Autor:Coll, T.A.; Chaufan, G.; Pérez-Tito, L.; Ventureira, M.R.; Sobarzo, C.M.A.; Ríos de Molina, M.D.C.; Cebral, E.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Autonoma de Buenos Aires, Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Ciudad Autonoma de Buenos Aires, Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Departamento de Química Biológica, Ciudad Autonoma de Buenos Aires, Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET), Ciudad Autonoma de Buenos Aires, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Medicina, CONICET- Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Ciudad Autonoma de Buenos Aires, Buenos Aires, Argentina
Palabras clave:apoptosis; mouse embryo organogenesis; nitrosylation; oxidative stress; perigestational alcohol; 3 nitrotyrosine; alcohol; caspase 3; catalase; drinking water; glutathione; nitrite; superoxide dismutase; thiobarbituric acid reactive substance; alcohol; antioxidant; adult; alcohol consumption; animal tissue; apoptosis; Article; caloric intake; cardiac muscle; cell adhesion; cell damage; controlled study; ectoderm; embryo; endocardium; enzyme activity; female; fetus development; fetus growth; immunohistochemistry; immunoreactivity; ingestion; lipid peroxidation; macromolecule; mesoderm; mouse; neural tube; nonhuman; organogenesis; oxidative stress; priority journal; tissue injury; TUNEL assay; Western blotting; animal; DNA damage; drinking behavior; drug effect; embryo development; genetics; mammalian embryo; metabolism; outbred strain; oxidative stress; pathology; pregnancy; prenatal exposure; Alcohol Drinking; Animals; Animals, Outbred Strains; Antioxidants; Apoptosis; DNA Damage; Embryo, Mammalian; Embryonic Development; Ethanol; Female; Mice; Organogenesis; Oxidative Stress; Pregnancy; Prenatal Exposure Delayed Effects
Año:2017
Volumen:84
Número:10
Página de inicio:1086
Página de fin:1099
DOI: http://dx.doi.org/10.1002/mrd.22865
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:3 nitrotyrosine, 3604-79-3; alcohol, 64-17-5; caspase 3, 169592-56-7; catalase, 9001-05-2; glutathione, 70-18-8; nitrite, 14797-65-0; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Ethanol
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v84_n10_p1086_Coll

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Citas:

---------- APA ----------
Coll, T.A., Chaufan, G., Pérez-Tito, L., Ventureira, M.R., Sobarzo, C.M.A., Ríos de Molina, M.D.C. & Cebral, E. (2017) . Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake. Molecular Reproduction and Development, 84(10), 1086-1099.
http://dx.doi.org/10.1002/mrd.22865
---------- CHICAGO ----------
Coll, T.A., Chaufan, G., Pérez-Tito, L., Ventureira, M.R., Sobarzo, C.M.A., Ríos de Molina, M.D.C., et al. "Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake" . Molecular Reproduction and Development 84, no. 10 (2017) : 1086-1099.
http://dx.doi.org/10.1002/mrd.22865
---------- MLA ----------
Coll, T.A., Chaufan, G., Pérez-Tito, L., Ventureira, M.R., Sobarzo, C.M.A., Ríos de Molina, M.D.C., et al. "Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake" . Molecular Reproduction and Development, vol. 84, no. 10, 2017, pp. 1086-1099.
http://dx.doi.org/10.1002/mrd.22865
---------- VANCOUVER ----------
Coll, T.A., Chaufan, G., Pérez-Tito, L., Ventureira, M.R., Sobarzo, C.M.A., Ríos de Molina, M.D.C., et al. Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake. Mol. Reprod. Dev. 2017;84(10):1086-1099.
http://dx.doi.org/10.1002/mrd.22865