Artículo

Scotti, L.; Parborell, F.; Irusta, G.; De Zuñiga, I.; Bisioli, C.; Pettorossi, H.; Tesone, M.; Abramovich, D. "Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients" (2014) Molecular Reproduction and Development. 81(8):748-756
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Abstract:

SUMMARY: Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growth factor (VEGF) are increased in PCOS patients. In this study, we examined the protein levels of platelet-derived growth factor (PDGF) BB and DD (PDGFBB and PDGFDD), angiopoietin 1 and 2 (ANGPT1 and ANGPT2), and their soluble receptor sTIE2 in FF from PCOS and control patients undergoing assisted reproductive techniques. We also analyzed the effect of FF from PCOS and control patients on tight and adherens junction protein expression in an endothelial cell line. PDGFBB and PDGFDD were significantly lower whereas ANGPT1 concentration was significantly higher in FF from PCOS patients than from control patients. No changes were found in the concentration of ANGPT2 or sTIE2. Expression of claudin-5 was significantly increased in endothelial cells incubated for 24hr in the presence of FF from PCOS versus from control patients, while vascular-endothelial cadherin, β-catenin, and zonula occludens 1 expression were unchanged. The changes observed in the levels of PDGF isoforms and ANGPT1 may prevent VEGF-induced vascular permeability in the PCOS ovary by regulating endothelial-cell-junction protein levels. Restoring the levels of angiogenic factors may provide new insights into PCOS treatment and the prevention of ovarian hyperstimulation syndrome in affected women. Mol. Reprod. Dev. 81: 748-756, 2014. © 2014 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients
Autor:Scotti, L.; Parborell, F.; Irusta, G.; De Zuñiga, I.; Bisioli, C.; Pettorossi, H.; Tesone, M.; Abramovich, D.
Filiación:Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina
Centro Médico PREGNA Medicina Reproductiva, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:angiopoietin 1; angiopoietin 2; angiopoietin receptor; beta catenin; claudin 5; estradiol; platelet derived growth factor; platelet derived growth factor BB; platelet derived growth factor DD; progesterone; protein ZO1; unclassified drug; vascular endothelial cadherin; vasculotropin; angiopoietin 1; angiopoietin 2; ANGPT1 protein, human; ANGPT2 protein, human; claudin 5; CLDN5 protein, human; estradiol; platelet derived growth factor B; platelet-derived growth factor BB; progesterone; vasculotropin A; adult; article; blood vessel permeability; cell junction; clinical article; cohort analysis; comparative study; controlled study; endothelium cell; enzyme linked immunosorbent assay; estradiol blood level; female; human; infertility therapy; oocyte; ovary follicle fluid; ovary polycystic disease; priority journal; progesterone blood level; protein blood level; protein expression; Western blotting; metabolism; Ovarian Hyperstimulation Syndrome; ovary; ovary follicle fluid; ovary polycystic disease; radioimmunoassay; tight junction; Adherens Junctions; Adult; Angiopoietin-1; Angiopoietin-2; Blotting, Western; Claudin-5; Endothelial Cells; Estradiol; Female; Follicular Fluid; Humans; Ovarian Hyperstimulation Syndrome; Ovary; Polycystic Ovary Syndrome; Progesterone; Proto-Oncogene Proteins c-sis; Radioimmunoassay; Reproductive Techniques, Assisted; Tight Junctions; Vascular Endothelial Growth Factor A
Año:2014
Volumen:81
Número:8
Página de inicio:748
Página de fin:756
DOI: http://dx.doi.org/10.1002/mrd.22343
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:angiopoietin 1, 186270-49-5; angiopoietin 2, 194368-66-6; estradiol, 50-28-2; progesterone, 57-83-0; vasculotropin, 127464-60-2; vasculotropin A, 489395-96-2; Angiopoietin-1; Angiopoietin-2; ANGPT1 protein, human; ANGPT2 protein, human; Claudin-5; CLDN5 protein, human; Estradiol; platelet-derived growth factor BB; Progesterone; Proto-Oncogene Proteins c-sis; Vascular Endothelial Growth Factor A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v81_n8_p748_Scotti

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Citas:

---------- APA ----------
Scotti, L., Parborell, F., Irusta, G., De Zuñiga, I., Bisioli, C., Pettorossi, H., Tesone, M.,..., Abramovich, D. (2014) . Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients. Molecular Reproduction and Development, 81(8), 748-756.
http://dx.doi.org/10.1002/mrd.22343
---------- CHICAGO ----------
Scotti, L., Parborell, F., Irusta, G., De Zuñiga, I., Bisioli, C., Pettorossi, H., et al. "Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients" . Molecular Reproduction and Development 81, no. 8 (2014) : 748-756.
http://dx.doi.org/10.1002/mrd.22343
---------- MLA ----------
Scotti, L., Parborell, F., Irusta, G., De Zuñiga, I., Bisioli, C., Pettorossi, H., et al. "Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients" . Molecular Reproduction and Development, vol. 81, no. 8, 2014, pp. 748-756.
http://dx.doi.org/10.1002/mrd.22343
---------- VANCOUVER ----------
Scotti, L., Parborell, F., Irusta, G., De Zuñiga, I., Bisioli, C., Pettorossi, H., et al. Platelet-derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients. Mol. Reprod. Dev. 2014;81(8):748-756.
http://dx.doi.org/10.1002/mrd.22343