Artículo

Buffone, M.G.; Brugo-Olmedo, S.; Calamera, J.C.; Verstraeten, S.V.; Urrutia, F.; Grippo, L.; Corbetta, J.P.; Doncel, G.F. "Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele" (2006) Molecular Reproduction and Development. 73(12):1591-1599
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Abstract:

Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity and protein tyrosine phosphorylation. Given the reported association between varicocele, oxidative stress, and sperm dysfunction, we hypothesized that spermatozoa from infertile patients with varicocele might have a combined defect at the level of membrane fluidity and protein tyrosine phosphorylation. Semen samples from infertile patients with and without grade II/III left varicocele were evaluated for motion parameters (computer-assisted semen analysis [CASA]), hyperactivation (CASA), incidence and intensity of protein tyrosine phosphorylation (phosphotyrosine immunofluorescence and western blotting), and membrane fluidity (Laurdan fluorometry), before and after a capacitating incubation (6 hr at 37°C in Ham's F10/BSA, 5% CO2). Spermatozoa from varicocele samples presented a decreased response to the capacitating challenge, showing significantly lower motility, hyperactivation, incidence and intensity of tyrosine phosphorylation, and membrane fluidity. The findings reported in this article indicate that the sperm dysfunction associated to infertile varicocele coexists with decreased sperm plasma membrane fluidity and tyrosine phosphorylation. These deficiencies represent potential new pathophysiological mechanisms underlying varicocele-related infertility. © 2006 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
Autor:Buffone, M.G.; Brugo-Olmedo, S.; Calamera, J.C.; Verstraeten, S.V.; Urrutia, F.; Grippo, L.; Corbetta, J.P.; Doncel, G.F.
Filiación:Laboratorio de Estudios en Reproducción (LER), Buenos Aires, Argentina
Centro de Estudios en Ginecología y Reproducción (CEGYR), Buenos Aires, Argentina
Departamento de Química Biológica, IQUIFIB (CONICET-UBA), Universidad de Buenos Aires, Buenos Aires, Argentina
Servicio de Urología, Hospital Francés, Buenos Aires, Argentina
Department of Obstetrics, Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA, United States
CONRAD Program, Department of Obstetrics, Eastern Virginia Medical School, 601 Colley Ave, Norfolk, VA 23507, United States
Palabras clave:Capacitation; Human spermatozoa; Hyperactivation; Membrane properties; Semen pathology; Signal transduction; protein tyrosine kinase; article; cell membrane fluidity; clinical article; computer analysis; enzyme phosphorylation; human; human cell; immunofluorescence; incidence; male; male infertility; pathophysiology; priority journal; semen analysis; spermatozoon; spermatozoon capacitation; spermatozoon motility; varicocele; Western blotting; Adult; Cell Membrane; Humans; Infertility, Male; Male; Membrane Fluidity; Phosphorylation; Protein-Tyrosine Kinases; Sperm Motility; Spermatozoa; Varicocele
Año:2006
Volumen:73
Número:12
Página de inicio:1591
Página de fin:1599
DOI: http://dx.doi.org/10.1002/mrd.20611
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:protein tyrosine kinase, 80449-02-1; Protein-Tyrosine Kinases, EC 2.7.1.112
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v73_n12_p1591_Buffone

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Citas:

---------- APA ----------
Buffone, M.G., Brugo-Olmedo, S., Calamera, J.C., Verstraeten, S.V., Urrutia, F., Grippo, L., Corbetta, J.P.,..., Doncel, G.F. (2006) . Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele. Molecular Reproduction and Development, 73(12), 1591-1599.
http://dx.doi.org/10.1002/mrd.20611
---------- CHICAGO ----------
Buffone, M.G., Brugo-Olmedo, S., Calamera, J.C., Verstraeten, S.V., Urrutia, F., Grippo, L., et al. "Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele" . Molecular Reproduction and Development 73, no. 12 (2006) : 1591-1599.
http://dx.doi.org/10.1002/mrd.20611
---------- MLA ----------
Buffone, M.G., Brugo-Olmedo, S., Calamera, J.C., Verstraeten, S.V., Urrutia, F., Grippo, L., et al. "Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele" . Molecular Reproduction and Development, vol. 73, no. 12, 2006, pp. 1591-1599.
http://dx.doi.org/10.1002/mrd.20611
---------- VANCOUVER ----------
Buffone, M.G., Brugo-Olmedo, S., Calamera, J.C., Verstraeten, S.V., Urrutia, F., Grippo, L., et al. Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele. Mol. Reprod. Dev. 2006;73(12):1591-1599.
http://dx.doi.org/10.1002/mrd.20611