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Abstract:

Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) has recently become as a putative modulator of sperm function. The aim of this study was to explore the presence of a GABAergic regulation of bovine sperm capacitation as well as the possible intracellular mechanisms involved. GABA was detected in fresh semen by a sensitive radioreceptor assay (spermatozoa, 0.064 ± 0.003 nmoles/106 cells; seminal plasma, 23.21 ± 1.16 nmoles/ml). Scatchard analysis of [3 H]-muscimol binding to sperm membranes yielded a linear plot consistent with a single population of binding sites (Kd = 3.87 nM, Bmax = 417 fmol/mg prot.). [3H]-muscimol specific binding to sperm membranes was significantly inhibited by the GABA A receptor (GABA A-R) antagonist bicuculline and by the agonists muscimol and isoguvacine. Addition of GABA to the incubation medium resulted in a concentration-dependent increase in the percentage of capacitated spermatozoa (chlortetracycline assay). We observed a significant increment on intracellular calcium and cyclic 3′,5′ adenosine monophosphate (cAMP) concentrations induced by GABA, being the cation influx abolished when the cell suspensions were coincubated with the antagonists bicuculline or picrotoxin. It is concluded that GABA induces sperm capacitation through an intracellular mechanism dependent on calcium influx and cAMP accumulation mediated by a specific GABA A-R. © 2004 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation
Autor:Ritta, M.N.; Bas, D.E.; Tartaglione, C.M.
Filiación:Inst. of Exp. Biology and Medicine, University of Lomas de Zamora, Buenos Aires, Argentina
School of Agrarian Sciences, University of Lomas de Zamora, Buenos Aires, Argentina
Inst. de Biol./Med. Exp.-CONICET, Obligado 2490, 1428-Buenos Aires, Argentina
Inst. of Biol. and Exp. Medicine, Obligado 2490-1428, Buenos Aires, Argentina
Palabras clave:Bovine; Capacitation; Chlortetracycline; GABA; Spermatozoa; 4 aminobutyric acid; arecaidine; bicuculline; cyclic AMP; muscimol; picrotoxin; animal cell; article; binding site; calcium cell level; cation transport; cattle; cell suspension; controlled study; fertilization; in vitro study; membrane binding; nonhuman; priority journal; radioreceptor assay; Scatchard plot; sperm; spermatozoon capacitation; spermatozoon maturation; Animals; Calcium; Cattle; Cyclic AMP; GABA Agonists; gamma-Aminobutyric Acid; Male; Protein Binding; Receptors, GABA-A; Sperm Capacitation; Spermatozoa; Bovinae
Año:2004
Volumen:67
Número:4
Página de inicio:478
Página de fin:486
DOI: http://dx.doi.org/10.1002/mrd.20038
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; arecaidine, 499-04-7; bicuculline, 485-49-4; cyclic AMP, 60-92-4; muscimol, 2763-96-4; picrotoxin, 124-87-8; Calcium, 7440-70-2; Cyclic AMP, 60-92-4; GABA Agonists; gamma-Aminobutyric Acid, 56-12-2; Receptors, GABA-A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v67_n4_p478_Ritta

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Citas:

---------- APA ----------
Ritta, M.N., Bas, D.E. & Tartaglione, C.M. (2004) . In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation. Molecular Reproduction and Development, 67(4), 478-486.
http://dx.doi.org/10.1002/mrd.20038
---------- CHICAGO ----------
Ritta, M.N., Bas, D.E., Tartaglione, C.M. "In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation" . Molecular Reproduction and Development 67, no. 4 (2004) : 478-486.
http://dx.doi.org/10.1002/mrd.20038
---------- MLA ----------
Ritta, M.N., Bas, D.E., Tartaglione, C.M. "In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation" . Molecular Reproduction and Development, vol. 67, no. 4, 2004, pp. 478-486.
http://dx.doi.org/10.1002/mrd.20038
---------- VANCOUVER ----------
Ritta, M.N., Bas, D.E., Tartaglione, C.M. In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation. Mol. Reprod. Dev. 2004;67(4):478-486.
http://dx.doi.org/10.1002/mrd.20038