Artículo

Chiappe, M.E.; Lattanzi, M.L.; Colman-Lerner, A.A.; Barañao, J.L.; Saragüeta, P. "Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development" (2002) Molecular Reproduction and Development. 61(2):135-141
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Abstract:

We analyzed the presence of 3β-Hydroxysteroid Dehydrogenase/Δ5→4-isomerase enzyme (3β-HSD) activity, a key enzyme of the steroid metabolic pathway, the mRNA of this enzyme, and the steroid metabolism in in vitro produced bovine embryos. 3β-HSD activity was detected in in vitro matured oocytes (74.4±1.4%), 1-cell (72.9±6.1%), 2-cell (61.8±7.4%), 8-cell (50±5%), morulae (50.8±2.6%), blastocysts (94.4±3%), and hatched blastocysts (100±0%) meanwhile the 4-cell stage showed a significant reduction (16.7±4.7%). When total embryonic RNA of different stages was subjected to RT-PCR assays, the mRNA of 3β-HSD was found to be present in all developmental stages of in vitro produced bovine embryos, from the oocyte to the blastocyst, with a marked decrease at the 4-cell stage. To determine whether the temporal pattern of enzyme activity was dependent on the maternal to zygotic transition, embryos were incubated in the presence of a transcription inhibitor, α-amanitin. The reappearance of the enzyme activity after the 4-cell stage was blocked in α-amanitin treated embryos, indicating the requirement of embryonic transcription. On the other hand, the embryonic steroid metabolism was tested by incubating blastocyst with tritiated pregnenolone. Analysis of the metabolites by TLC indicated the production of a compound with a mobility identical to progesterone. These results described the expression of the 3β-HSD and the activity of this metabolic enzyme in bovine oocytes and preimplantation embryos, suggesting that steroids may act as autocrine effectors on preimplantation embryo development. © 2002 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development
Autor:Chiappe, M.E.; Lattanzi, M.L.; Colman-Lerner, A.A.; Barañao, J.L.; Saragüeta, P.
Filiación:Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina
Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Bovine; Embryo development; Gene expression; Steroids; 3(or 17)beta hydroxysteroid dehydrogenase; alpha amanitin; aromatase inhibitor; transcription factor; animal tissue; article; blastocyst; cattle; embryo development; enzyme activity; gene expression regulation; messenger RNA synthesis; nonhuman; phase transition; pregnancy; preimplantation embryo; priority journal; reverse transcription polymerase chain reaction; steroid metabolism; steroidogenesis; 3-Hydroxysteroid Dehydrogenases; Amanitins; Animals; Blastocyst; Cattle; Cells, Cultured; Dehydroepiandrosterone; Embryonic and Fetal Development; Female; Fertilization in Vitro; Nucleic Acid Synthesis Inhibitors; Oocytes; Pregnancy; Pregnenolone; Progesterone; RNA, Messenger; Bovinae
Año:2002
Volumen:61
Número:2
Página de inicio:135
Página de fin:141
DOI: http://dx.doi.org/10.1002/mrd.1140
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:3-Hydroxysteroid Dehydrogenases, EC 1.1.-; Amanitins; Dehydroepiandrosterone, 53-43-0; Nucleic Acid Synthesis Inhibitors; Pregnenolone, 145-13-1; Progesterone, 57-83-0; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v61_n2_p135_Chiappe

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Citas:

---------- APA ----------
Chiappe, M.E., Lattanzi, M.L., Colman-Lerner, A.A., Barañao, J.L. & Saragüeta, P. (2002) . Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development. Molecular Reproduction and Development, 61(2), 135-141.
http://dx.doi.org/10.1002/mrd.1140
---------- CHICAGO ----------
Chiappe, M.E., Lattanzi, M.L., Colman-Lerner, A.A., Barañao, J.L., Saragüeta, P. "Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development" . Molecular Reproduction and Development 61, no. 2 (2002) : 135-141.
http://dx.doi.org/10.1002/mrd.1140
---------- MLA ----------
Chiappe, M.E., Lattanzi, M.L., Colman-Lerner, A.A., Barañao, J.L., Saragüeta, P. "Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development" . Molecular Reproduction and Development, vol. 61, no. 2, 2002, pp. 135-141.
http://dx.doi.org/10.1002/mrd.1140
---------- VANCOUVER ----------
Chiappe, M.E., Lattanzi, M.L., Colman-Lerner, A.A., Barañao, J.L., Saragüeta, P. Expression of 3β-hydroxysteroid dehydrogenase in early bovine embryo development. Mol. Reprod. Dev. 2002;61(2):135-141.
http://dx.doi.org/10.1002/mrd.1140