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Abstract:

Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relationship of this interaction with the behavior and function of the protein. Cloning and sequencing of DE revealed a lack of hydrophobic domains and the presence of 16 cysteine residues in the molecule. Experiments in which cauda epididymal sperm were subjected to different extraction procedures indicated that while most of the protein is removable from sperm by mild ionic strength, a low amount of DE, resistant to even 2 M NaCl, can be completely extracted by agents that remove integral proteins. However, the lack of hydrophobic domains in the molecule and the failure of DE to interact with liposomes, does not support a direct insertion of the protein into the lipid bilayer. These results, and the complete extraction of the tightly bound protein by dithiothreitol, suggest that this population would correspond to a peripheral protein bound to a membrane component by strong noncovalent interactions that involve disulfide bonds. While ELISA experiments showed that no protein could be extracted by NaCl from capacitated sperm, indirect immunofluorescence studies revealed the ability of the NaCl-resistant protein to migrate to the ES. Together, these results support the existence of two populations of DE: a major, loosely bound population that is released during capacitation, and a minor strongly bound population that remains after capacitation, migrates to the ES with the AR, and thus would correspond to the one with a role in gamete fusion. (C) 2000 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Relationship between the association of rat epididymal protein 'DE' with spermatozoa and the behavior and function of the protein
Autor:Cohen, D.J.; Rochwerger, L.; Ellerman, D.A.; Morgenfeld, M.M.; Busso, D.; Cuasnicú, P.S.
Filiación:Inst. de Biología y Med. Exp., Buenos Aires, Argentina
Inst. de Biología y Med. Exp., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Capacitation; Fertilization; Maturation; Sperm membrane; dithiothreitol; glycoprotein; glycoprotein de; isoprotein; liposome; sodium chloride; unclassified drug; animal cell; article; cell adhesion; controlled study; disulfide bond; epididymis tail; hydrophobicity; male; nonhuman; priority journal; protein binding; protein domain; protein isolation; protein lipid interaction; protein transport; rat; reaction analysis; sequence analysis; spermatozoon; spermatozoon maturation; Animals; Cell Membrane; Cloning, Molecular; Epididymal Secretory Proteins; Male; Metalloproteins; Rats; Rats, Sprague-Dawley; Sperm Capacitation; Spermatozoa; Testicular Hormones; Animalia
Año:2000
Volumen:56
Número:2
Página de inicio:180
Página de fin:188
DOI: http://dx.doi.org/10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:Epididymal Secretory Proteins; Metalloproteins; Testicular Hormones
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v56_n2_p180_Cohen

Referencias:

  • Bavister, B.D., A consistently successful procedure for in vitro fertilization of golden hamster eggs (1989) Gamete Res, 23, pp. 139-158
  • Bedford, J.M., Sperm dynamics in the epididymis (1990) Gamete Physiology, pp. 53-68. , Asch RH, Balmaceda JP, Johnson I, editors. Norwell, MA: Serono Symposium
  • Bedford, J.M., Moore, H.D.M., Franklin, L.E., Significance of the equatorial segment of the acrosome of the spermatozoon in eutherian mammals (1979) Exp Cell Res, 119, pp. 119-126
  • Bérubé, B., Lefièvre, L., Coutu, L., Sullivan, R., Regulation of the epididymal synthesis of P26h, a hamster sperm protein (1996) J Androl, 17, pp. 104-110
  • Blobel, C.P., Myles, D., Primakoff, P., White, J., Proteolytic processing of a protein involved in sperm-egg fusion correlates with acquisition of fertilization competence (1990) J Cell Biol, 111, pp. 69-78
  • Boué, F., Blais, J., Sullivan, R., Surface localization of P34H, an epididymal protein, during maturation, capacitation, and acrosome reaction of human spermatozoa (1996) Biol Reprod, 54, pp. 1009-1017
  • Brooks, D.E., Androgen-regulated epididymal secretory proteins associated with post-testicular sperm development (1987) Ann New York Acad Sci, 513, pp. 179-194
  • Brooks, D.E., Higgins, S.J., Characterization and androgen-dependence of proteins associated with luminal fluid and spermatozoa in the rat epididymis (1980) J Reprod Fertil, 59, pp. 363-375
  • Brooks, D.E., Means, A.R., Wright, E.J., Singh, S.P., Tiver, K.K., Molecular cloning of the cDNA for androgen-dependent sperm-coating glycoproteins secreted by the rat epididymis (1986) Eur J Biochem, 161, pp. 13-18
  • Cameo, M.S., Blaquier, J.A., Androgen-controlled specific proteins in rat epididymis (1976) J Endocr, 69, pp. 317-324
  • Cameo, M.S., Gonzalez Echeverria, M.F., Blaquier, J.A., Burgos, M.H., Immunochemical localization of epididymal protein DE on rat spermatozoa: Its fate after induced acrosome reaction (1986) Gam Res, 15, pp. 247-258
  • Charest, N.J., Joseph, D.R., Wilson, E.M., French, F.S., Molecular cloning of complementary deoxyribonucleic acid for an androgen-regulated epididymal protein: Sequence homology with metalloproteins (1988) Mol Endo, 2, pp. 999-1004
  • Cohen, D.J., Munuce, M.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: The development of rat oolemma fusibility during oogenesis involves the appearance of binding sites for sperm protein "DE." (1996) Biol Reprod, 55, pp. 200-206
  • Cooper, T.G., (1986) The Epididymis, Sperm Maturation, and Fertilization, , Heidelberg, Germany: Springer Verlag
  • Cuasnicú, P.S., Gonzalez Echeverria, F., Piazza, A., Piñeiro, L., Blaquier, J.A., Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa (1984) J Reprod Fertil, 71, pp. 427-431
  • Eberspaecher, U., Roosterman, D., Krätzschmar, J., Haendler, B., Habenicht, U.F., Becker, A., Quensel, C., Kratzschmar, J., Mouse androgen-dependent epididymal glycoprotein CRISP-1 (DE/AEG): Isolation, biochemical characterization, and expression in recombinant form (1995) Mol Reprod Dev, 42, pp. 157-172
  • Fang, K.S., Vitale, M., Fehlner, P., King, T.P., cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5 (1988) Proc Natl Acad Sci USA, 62, pp. 247-265
  • Foster, J.A., Gerton, G.L., Autoantigen l of the guinea pig sperm acrosome is the homologue of mouse Tpx-1 and human TPX1 and is a member of the cysteine-rich secretory protein (CRISP) family (1996) Mol Reprod Dev, 44, pp. 221-229
  • Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A., Isolation and characterization of specific rat epididymal proteins (1979) Mol Cell Endocrinol, 13, pp. 73-82
  • Garberi, J.C., Fontana, J.D., Blaquier, J.A., Carbohydrate composition of specific rat epididymal protein (1982) Int J Androl, 5, pp. 619-626
  • Haendler, B., Kratzschmar, J., Theuring, F., Schleuning, W.D., Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/ AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland (1993) Endocrinology, 133, pp. 192-198
  • Hames, B.D., An introduction to polyacrilamide gel electrophoresis (1981) Gel Electrophoresis of Proteins, pp. 1-88. , Hames BD, Rickwood D, editors. Oxford, Washington, DC: IRL Press
  • Hayashi, M., Fujimoto, S., Takano, H., Ushiki, T., Abe, K., Ishikura, H., Yoshida, M., Kasahara, M., Characterization of a human glycoprotein with potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1) (1996) Genomics, 32, pp. 367-374
  • Jones, R., Shalgi, R., Holland, J., Phillips, D.M., Topographical rearrangement of a plasma membrane antigen during capacitation of rat spermatozoa in vitro (1990) Dev Biol, 139, pp. 349-362
  • Kaplan, R., Kraicer, P.F., Effect of elevated calcium concentration on fertilization of rat oocytes in vitro (1978) Gam Res, 1, pp. 281-285
  • Kasahara, M., Gutknecht, J., Brew, K., Spurr, N., Goodfellow, P.N., Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene (1989) Genomics, 5, pp. 527-534
  • Kjeldsen, L., Cowland, J.B., Johnsen, A.H., Borregaard, N., SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein (1996) FEBS Lett, 380, pp. 246-250
  • Kohane, A.C., Garberi, J.C., Cameo, M.S., Blaquier, J.A., Quantitative determination of specific proteins in rat epididymis (1979) J Steroid Biochem, 11, pp. 671-674
  • Kohane, A.C., Cameo, M.S., Piñeiro, L., Garberi, J.C., Blaquier, J.A., Distribution and site of production of specific proteins in the rat epididymis (1980) Biol Reprod, 23, pp. 181-187
  • Kohane, A.C., Gonzalez Echeverria, F., Piñeiro, L., Blaquier, J.A., Interaction of proteins of epididymal origin with spermatozoa (1980) Biol Reprod, 23, pp. 737-742
  • Kohane, A.C., Pineiro, L., Blaquier, J.A., Androgen-controlled synthesis of specific protein in the rat epididymis (1983) Endocrinology, 112, pp. 1590-1596
  • Kratzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., Schleuning, W.D., The human cysteine-rich secretory protein (CRISP) family: Primary structure and tissue distribution of CRISP-1, CRISP-2, and CRISP-3 (1996) Eur J Biochem, 236, pp. 827-836
  • Lea, O.A., Petrusz, P., French, F.S., Purification and localization of acidic apididymal glycoprotein (AEG): A sperm coating secreted by the rat epididymis (1978) Int Androl Suppl, 2, pp. 592-607
  • Maeda, T., Sakashita, M., Ohba, Y., Nakanishi, Y., Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells (1998) Biochem Biophys Res Commun, 248, pp. 140-146
  • Mizuki, N., Kasahara, M., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule (1992) Mol Cell Endocrinol, 89, pp. 25-32
  • Moore, A., Ensrud, K.M., White, T.W., Frethem, C.D., Hamilton, D.W., Rat epididymis-specific sperm maturation antigens: I. Evidence that the 26 kD 4E9 antigen found on rat caudal epididymal sperm tail is derived from a protein secreted by the epididymis (1994) Mol Reprod Dev, 37, pp. 181-194
  • Moore, H.D.M., The influence of the epididymis on human and animal sperm maturation and storage (1996) Hum Reprod, 11, pp. 103-110
  • Morrissette, J., Kratzschmar, J., Haendler, B., El-Hayek, R., Mochca-Morales, J., Martin, B.M., Jitandrakumar, R.P., Possani, L.D., Primary structure and properties of helothermine, a peptide that blocks ryanodine receptors (1995) Biophys J, 68, pp. 2280-2288
  • Orgebin-Crist, M.C., Fournier-Delpech, S., Sperm-egg interaction: Evidence for maturational changes during epididymal transit (1982) J Androl, 3, pp. 429-433
  • Phelps, B.M., Myles, D.G., The guinea pig sperm plasma membrane protein, pH-20, reaches the surface via two transport pathways and becomes localized to a domain after an initial uniform distribution (1987) Dev Biol, 123, pp. 63-72
  • Rankin, T.L., Tsuruta, K.J., Holland, M.K., Griswold, M.D., Orgebin-Crist, M.C., Isolation, immunolocalization, and sperm-association of three proteins of 18, 25, and 29 kilodaltons secreted by the mouse epididymis (1992) Biol Reprod, 46, pp. 747-766
  • Rigden, J., Coutts, R., Pathogenesis-related proteins in plants (1988) Trends Genet, 4, pp. 87-89
  • Rochwerger, L., Cuasnicú, P.S., Redistribution of a rat sperm epididymal glycoprotein after in vivo and in vitro capacitation (1992) Mol Reprod Dev, 31, pp. 34-41
  • Rochwerger, L., Cohen, D.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion (1992) Dev Biol, 153, pp. 83-90
  • Sanjurjo, C., Dawidowsky, A.R., Cameo, M.S., Gonzalez Echeverria, F., Blaquier, J.A., Participation of human epididymal sperm coating antigens in fertilization (1990) J Androl, 11, pp. 476-483
  • Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrilamyde gels to nitrocellulose sheets: Procedure and some applications (1979) Proc Natl Acad Sci USA, 76, pp. 4350-4360
  • Wong, P.Y.D., Tsang, A.Y.F., Studies on the binding of a 32K rat epididymal protein to rat epididymal spermatozoa (1982) Biol Reprod, 27, pp. 1239-1246
  • Wong, P.Y.D., Tsang, A.Y.F., Lee, W.M., Origin of the luminal fluid proteins of the rat epididymis (1981) Int J Androl, 4, pp. 331-341
  • Xu, W., Hamilton, D.W., Identification of the rat epididymis-secreted 4E9 antigen as protein E: Further biochemical characterization of highly homologous epididymal secretory proteins D and E (1996) Mol Reprod Dev, 43, pp. 347-357
  • Xu, W., Ensrud, K.M., Hamilton, D.W., The 26 kD protein recognized on rat cauda epididymal sperm by monoclonal antibody 4E9 has internal peptide sequence that is identical to the secreted form of epididymal protein E (1997) Mol Reprod Dev, 46, pp. 377-382
  • Yanagimachi, R., Sperm-egg fusion (1988) Current Topics in Membranes and Transport, pp. 3-43. , Duzgunes N, Bronner F, editors. Orlando, FL. Academic Press
  • Yanagimachi, R., Mammalian fertilization (1994) The Physiology of Reproduction, pp. 189-317. , Knobil E, Neill JD, editors. New York: Raven Press

Citas:

---------- APA ----------
Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M.M., Busso, D. & Cuasnicú, P.S. (2000) . Relationship between the association of rat epididymal protein 'DE' with spermatozoa and the behavior and function of the protein. Molecular Reproduction and Development, 56(2), 180-188.
http://dx.doi.org/10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4
---------- CHICAGO ----------
Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M.M., Busso, D., Cuasnicú, P.S. "Relationship between the association of rat epididymal protein 'DE' with spermatozoa and the behavior and function of the protein" . Molecular Reproduction and Development 56, no. 2 (2000) : 180-188.
http://dx.doi.org/10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4
---------- MLA ----------
Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M.M., Busso, D., Cuasnicú, P.S. "Relationship between the association of rat epididymal protein 'DE' with spermatozoa and the behavior and function of the protein" . Molecular Reproduction and Development, vol. 56, no. 2, 2000, pp. 180-188.
http://dx.doi.org/10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4
---------- VANCOUVER ----------
Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M.M., Busso, D., Cuasnicú, P.S. Relationship between the association of rat epididymal protein 'DE' with spermatozoa and the behavior and function of the protein. Mol. Reprod. Dev. 2000;56(2):180-188.
http://dx.doi.org/10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>3.0.CO;2-4