Artículo

Saragüeta, P.; Lanuza, G.; V. Miranda, P.; G. Tezón, J.; Lino Baraño, J. "Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm" (1994) Molecular Reproduction and Development. 39(3):280-288
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR‐inducing activity. AR activity was associated with a protein fraction of Mr > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent Mr 50,000 and 29,000. The N‐terminal sequences of these bands showed a 100% homology with the heavy and light chains of human lgG. A polyclonal antibody raised against the purified protein and anti‐human lgG were both able to suppress the acrosome reactioninducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human lgG were able to mimic the AR‐inducing activity of hFF. We concluded that the AR‐inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc.

Registro:

Documento: Artículo
Título:Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
Autor:Saragüeta, P.; Lanuza, G.; V. Miranda, P.; G. Tezón, J.; Lino Baraño, J.
Filiación:Instituto de Biología Y Medicina Experimental, Conicet, Facultad de Ciencias Exactas Y Naturales, U.B.A., Buenos Aires, Argentina
Palabras clave:Acrosome reaction; Antibodies; Follicular fluid; Sperm; immunoglobulin g; immunoglobulin heavy chain; immunoglobulin light chain; protein subunit; sperm antibody; acrosome; amino terminal sequence; article; controlled study; female; fertilization in vitro; human; human cell; male; ovary follicle fluid; polyacrylamide gel electrophoresis; priority journal; sequence homology; Acrosome; Amino Acid Sequence; Antibodies; Female; Follicular Fluid; Human; Male; Molecular Sequence Data; Molecular Weight; Sperm Immobilizing Agents; Sperm-Ovum Interactions; Spermatozoa; Support, Non-U.S. Gov't
Año:1994
Volumen:39
Número:3
Página de inicio:280
Página de fin:288
DOI: http://dx.doi.org/10.1002/mrd.1080390305
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CAS:Antibodies; Sperm Immobilizing Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v39_n3_p280_Saragueta

Referencias:

  • Bandoh, R, Daitoh, T, Yamano, S, Aono, T, Kamada, M, Effect of sperm‐immobilizing antibodies on the acrosome reaction of human spermatozoa (1992) Fertil Steril, 57, pp. 387-392
  • Blackmore, PF, Lattanzio, FA, Cell surface localization of a novel non‐genomic progesterone receptor on the head of human sperm (1991) Biochem Biophys Res Commun, 181, pp. 331-336
  • Blackmore, PF, Neulen, J, Lattanzio, F, Beebe, SJ, Cell surfacebinding sites for protesterone mediate calcium uptake in human sperm (1991) J Biol Chem, 266, pp. 18655-18659
  • Bradford, MM, Rapid and sensitive method for the quantitation of micrograms quantities of protein utilising the principle of protein‐dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Calvo, L, Koukoulis, GN, Vantman, D, Dennison, L, Banks, SM, Sherins, RJ, Tezon, J, Follicular fluid‐induced acrosome reaction distinguishes a subgroup of men with unexplained infertility not identified by semen analysis (1989) Fertil Steril, 52, pp. 1048-1054
  • Carron, CP, Anti‐idiotypic antibodies as immunizing antigens for immunocontraception (1990) Gamete Interaction. Prospects for Immunocontraception, pp. 401-414. , NJ Alexander, D Griffin, JM Spieler, GMH Waites, (eds):, New York, Wiley‐Liss
  • Cross, NL, Maizel, S, Methods for evaluating the acrosomal status of mammalian sperm (1989) Biol Reprod, 41, pp. 635-641
  • Cross, NL, Morales, P, Overstreet, JW, Hanson, FW, Two simple methods for detecting acrosome reacted human sperm (1986) Gamete Res, 15, pp. 213-226
  • Cross, NL, Morales, P, Overstreet, JW, Hanson, FW, Induction of acrosome reactions by the human zona pellucida (1988) Biol Reprod, 38. , 235, 230
  • Curtis, P, Burford, G, Amso, N, Keith, E, Shaw, RW, Assessment of relevance of zona pellucida antibodies in serum and cervical mucus in patients who have fertilization failure during in vitro fertilization (1991) Fertil Steril, 56, pp. 1124-1127
  • Dravland, JE, Llancs, MN, Munn, RJ, Meizel, S, Evidence for the involvement of asperm trypsinlike enzyme in the membrane events of the hamster sperm acrosome reaction (1984) J Exp Zool, 232, pp. 117-128
  • Falcone, L, Marchini, M, Gianni, S, Eppenberger, U, Piffaretti‐Yanez, A, Balerna, M, Follicular fluid enhances sperm motility and velocity in vitro (1991) Fertil Steril, 55, pp. 619-623
  • Fann, C‐H, Lee, C‐YG, Monoclonal antibodies affecting sperm–zona binding and/or zona‐induced acrosome reaction (1992) J Reprod Immunol, 21, pp. 175-187
  • Garbers, DL, Molecular basis of fertilization (1989) Annu Rev Biochem, 58, pp. 719-742
  • Ghetler, Y, Jaffe, R, Ben‐Nun, I, Gruber, A, Kaneti, H, Fejgin, M, Effect of sperm preincubation with follicular fluid on the fertilization rate in human in vitro fertilization (1990) Fertil Steril, 54, pp. 944-946
  • Goldman, D, Sedman, SA, Ebert, MH, Ultrasensitive stain for proteins in polyacrilamide gels show regional variation in cerebrospinal fluid proteins (1981) Science, 211, pp. 1437-1438
  • Harlow, E, Lane, D, Storing and purifying antibodies (1988) Antibodies: A Laboratory Manual, pp. 283-318. , Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
  • Kamada, M, Liang, Z, Koide, SS, Identification of IgG and Fcbinding proteins in human seminal plasma and sperm (1991) Arch Androl, 27, pp. 1-7
  • Laemmli, UK, Cleavage of structural proteins during the assembly of the heads of bacteriophage T4 (1970) Nature, 277, pp. 680-685
  • Lansford, B, Haas, GG, Jr, Debault, LE, Wolf, DP, Effect of spermassociated antibodies on the acrosomal status of human sperm (1990) J Androl, 11, pp. 532-538
  • Leyton, L, Saling, PM, Evidence that aggregation of sperm receptors by ZP3 triggers the acrosome reaction (1989) J Cell Biol, 108, pp. 2163-2168
  • Macek, MB, Lopez, LC, Shur, BD, Aggregation of β‐1,4‐galactosyltransferase on mouse sperm induces the acrosome reaction (1991) Dev Biol, 147, pp. 440-444
  • Mantzavinos, T, Dalamanga, N, Dimitriadou, F, Konidaris, S, Gregoriou, O, Zourlas, PA, (1991), Autoantibodies to zona pellucida in serum and follicular fluid in IVF patients. Abstracts of the Seventh Annual meeting of the ESHRE, Paris 102 (abst); Margalioth, EJ, Scholl, GM, Cooper, GW, Rosenfeld, DL, Taney, FH, Capacitated sperm cells react with different types of antisperm antibodies than fresh ejaculated sperm (1992) Fertil Steril, 57, pp. 393-398
  • Mbizvo, MT, Burkman, LJ, Alexander, NJ, Human follicular fluid stimulates hyperactivated motility in human sperm (1990) Fertil Steril, 54, pp. 708-712
  • McClure, RD, Tom, RA, Dandekar, PV, Optimizing the sperm penetration assay with human follicular fluid (1990) Fertil Steril, 53, pp. 546-550
  • Meizel, S, Pillai, MC, Diaz‐Perez, E, Thomas, P, Initiation of the human sperm acrosome reaction by components of human follicular fluid and cumulus secretions including steroids (1990) Fertilization in Mammals, pp. 205-222. , BD Bavister, J Cummins, ERS Roldan, (eds):, Norwell, MA, Serono Symposia
  • Morales, P, Llanos, M, Gutierrez, G, Kohen, P, Vigil, P, Vantman, D, The acrosome reaction‐inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content (1992) Hum Reprod, 7, pp. 646-651
  • Mortimer, D, Camenzind, AR, The role of follicular fluid in inducing the acrosome reaction of human spermatozoa incubated in vitro (1989) Hum Reprod, 4, pp. 169-175
  • Muller, CH, Ravnik, SE, Zarutskie, PW, Follicular fluid enhanced sperm penetration assay: Use in diagnosis of sperm dysfunction (1990) J Androl, 11, p. 42. , P (abst)
  • Osman, RA, Andria, ML, Jones, AD, Meizel, S, Steroid induced exocytosis: The human sperm acrosome reaction (1989) Biochem Biophys Res Commun, 160, pp. 828-833
  • Ralt, D, Goldenberg, M, Fetterolf, P, Thompson, D, Dor, J, Mashiach, S, Garbers, DL, Eisenbach, M, Sperm attraction to a follicular factor(s) correlates with human egg fertilizability (1991) Proc Natl Acad Sci USA, 88, pp. 2840-2844
  • Ravnik, SE, Zarutskie, PW, Muller, CH, Lipid transfer activity in human follicular fluid: Relation to human sperm capacitation (1990) J Androl, 11, pp. 216-226
  • Revelli, A, Massobrio, M, Soldati, G, Töpfer‐Petersen, E, Stamm, J, Balerna, M, Effect of volumetric mixtures of peritoneal and follicular fluid from the same woman on sperm motility and acrosomal reactivity in vitro (1992) Fertil Steril, 57, pp. 654-660
  • Rhim, SH, Millar, SE, Robey, F, Luo, A‐M, Lou, Y‐H, Yule, T, Allen, P, Tung, KSK, Autoimmune disease of the ovary induced by a ZP3 peptide from the mouse zona pellucida (1992) J Clin Invest, 89, pp. 28-35
  • Roldan, ERS, Physiological stimulators of the acrosome reaction (1990) Fertilization in mammals, pp. 197-204. , BD Bavister, J Cummins, ERS Roldan, (eds):, Norwell, MA, Serono Symposia
  • Sege, K, Peterson, PA, Anti‐idiotypic antibodies as cell surface receptor probes (1978) Proc Natl Acad Sci USA, 75, pp. 2443-2447
  • Silverberg, KM, Dey, T, Olive, DL, Schenken, RS, Sperm antibodies facilitate the acrosome reaction in human spermatozoa in vitro (1992) Fertil Steril, 58. , 21, S, (abst)
  • Suarez, SS, Wolf, DP, Meizel, S, Induction of the acrosomal reaction in human spermatozoa by a fraction of human follicular fluid (1986) Gamete Res, 14, pp. 107-121
  • Tesarik, J, Mendoza, C, Insights into the function of a spermsurface progesterone receptor: Evidence of ligand‐induced receptor aggregation and the implication of proteolysis (1993) Exp Cell Res, 205, pp. 111-117
  • Thomas, P, Meizel, S, An influx of extracellular calcium is required for initiation of the human sperm acrosome reaction induced by human follicular fluid (1988) Gamete Res, 20, pp. 397-413
  • Towbin, H, Staehlin, T, Gordon, J, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) Proc Natl Acad Sci USA, 76, pp. 4350-4360
  • Vaitukaitis, J, Robbins, JB, Nieschlag, E, Ross, GT, A method for producing specific antisera with small doses of immunogen: Multiple intradermal injections (1971) J Clin Endocrinol Metab, 33, pp. 988-991
  • Villaneuva‐Diaz, C, De los Angeles, Vadillo‐Ortega, F, Krivitzky, SK, Kably‐Ambe, A, Evidence that human follicular fluid contains a chemoattractant for spermatozoa (1990) Fertil Steril, 54, pp. 1180-1182
  • White, DR, Phillips, DM, Bedford, JM, Factors affecting the acrosome reaction in human spermatozoa (1990) J Reprod Fertil, 90, pp. 71-80
  • (1992) WHO Laboratory Manual for the Examination of Human Semen and Sperm Cervical Mucus Interaction, , New York, Cambridge University Press
  • Yanagimachi, R, Mammalian Fertilization (1988) The Physiology of Reproduction, pp. 135-185. , E Knobil, J Neill, (eds):, New York, Raven Press

Citas:

---------- APA ----------
Saragüeta, P., Lanuza, G., V. Miranda, P., G. Tezón, J. & Lino Baraño, J. (1994) . Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm. Molecular Reproduction and Development, 39(3), 280-288.
http://dx.doi.org/10.1002/mrd.1080390305
---------- CHICAGO ----------
Saragüeta, P., Lanuza, G., V. Miranda, P., G. Tezón, J., Lino Baraño, J. "Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm" . Molecular Reproduction and Development 39, no. 3 (1994) : 280-288.
http://dx.doi.org/10.1002/mrd.1080390305
---------- MLA ----------
Saragüeta, P., Lanuza, G., V. Miranda, P., G. Tezón, J., Lino Baraño, J. "Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm" . Molecular Reproduction and Development, vol. 39, no. 3, 1994, pp. 280-288.
http://dx.doi.org/10.1002/mrd.1080390305
---------- VANCOUVER ----------
Saragüeta, P., Lanuza, G., V. Miranda, P., G. Tezón, J., Lino Baraño, J. Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm. Mol. Reprod. Dev. 1994;39(3):280-288.
http://dx.doi.org/10.1002/mrd.1080390305