Artículo
Abstract:
In this work, two well known polymers, carboxymethyl dextran and poly(ethyleneglycol), are used for easily producing a platform for electrochemical affinity assays, avoiding nonspecific adsorption of proteins. In this way, modified electrode surfaces able to bind a recognition agent are constructed through simple and reliable reactions. 24 and 70 KDa Carboxymethyl Dextrans (CMDex24 and CMDex70) were covalently attached to gold through 2-aminoethylthiol, allowing the fast electron transfer reaction of a redox mediator. Based on ellipsometry and amperometry results, the nonspecific adsorption of neutravidin-horseradish peroxidase (NHRP), a conjugate used as indicating molecule, was studied considering the physicochemical characteristics of the CMDex films. The experimental results show that CMDex70 produces a dense structure reducing drastically the nonspecific adsorption of NHRP, still allowing the access of the redox mediator. Poly(ethylene glycol) derivates, combined with CMDex, were also investigated. Methoxypolyethylene glycol succinate N-hydroxysuccinimide ester (MW: 5000 Da), combined with either CMDex 24 or 70, was found also to reduce the nonspecific interactions. Additionally, the specific binding of NHRP, and the effect of the film structure in the amperometric response, were established using electrodes modified covalently with the specific biotin counterpart. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Registro:
| Documento: | Artículo |
| Título: | A protein-resistant matrix for electrochemical based recognition assays |
| Autor: | Pallarola, D.; Domenianni, L.; Priano, G.; Battaglini, F. |
| Filiación: | INQUIMAE, Departamento de Química Inorgánica, Analítica Y Química Física, Pabellón 2, C1428EHA Buenos Aires, Argentina |
| Palabras clave: | Amperometry; Dextran derivatives; Neutravidin; Nonspecific adsorption; Peroxidase; Proteins |
| Año: | 2007 |
| Volumen: | 19 |
| Número: | 6 |
| Página de inicio: | 690 |
| Página de fin: | 697 |
| DOI: | http://dx.doi.org/10.1002/elan.200603801 |
| Título revista: | Electroanalysis |
| Título revista abreviado: | Electroanalysis |
| ISSN: | 10400397 |
| CODEN: | ELANE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v19_n6_p690_Pallarola |
Referencias:
- Senaratne, W., Andruzzi, L., Ober, C.K., (2005) Biomacromol, 6, p. 2427
- Bi, H., Zhong, W., Meng, S., Kong, J., Yang, P., Liu, B., (2006) Anal. Chem, 78, p. 3399
- Wang, A.J., Xu, J.J., Chen, H.Y., (2006) Anal. Chim. Acta, 569, p. 188
- Shin, D., Tryk, D.A., Fujishima, A., Merkoçi, A., Wang, J., (2004) Electroanalysis, 17, p. 305
- Liu, J., Sun, X., Lee, M.L., (2005) Anal. Chem, 77, p. 6280
- Uchida, K., Otsuka, H., Kaneko, M., Kataoka, K., Nagasaki, Y., (2005) Anal. Chem, 77, p. 1075
- Masson, J.-F., Battaglia, T.M., Davidson, M.J., Kim, Y.-C., Prakash, A.M.C., Beaudoin, S., Booksh, K.S., (2005) Talanta, 67, p. 918
- Österberg, E., Bergström, K., Holmberg, K., Riggs, J.A., Van Alstine, J.M., Schuman, T.P., Burns, N.L., Harris, J.M., (1993) Colloids Surf. A: Physicochem. Eng. Aspects, 77, p. 159
- De Sousa Delgado, A., Léonard, M., Dellacherie, E., (2001) Langmuir, 17, p. 4386
- Löfas, S., Johnsson, B., (1990) J. Chem. Soc. Chem. Commun, 21, p. 1526
- Johnsson, B., Löfas, S., Lindqvist, G., (1991) Anal. Biochem, 198, p. 268
- Limoges, B., Savéant, J.M., Yazidi, D., (2003) J. Am. Chem. Soc, 125, p. 9192
- Rogan, G.J., Dudin, Y.A., Lee, T.C., Magin, K.M., Astwood, J.D., Bhakta, N.S., Leach, J.N., Fuchs, R.L., (1999) Food Control, 10, p. 407
- Robinson, S.A., Rosenzweig, S.A., (2004) Biochem, 43, p. 11533
- Kober, E.M., Caspar, J.V., Sullivan, B.P., Meyer, T.J., (1988) Inorg. Chem, 27, p. 4587
- Hattori, M., Nagasawa, K., Ametani, A., Kaminogawa, S., Takahashit, K., (1994) J. Agric. Food Chem, 42, p. 2120
- Kobayasi, K., Yoshida, T., Takahashi, K., Hattori, M., (2003) Biocon jugate Chem, 14, p. 168
- Dequaire, M., Limoges, B., Moiroux, J., Savéant, J.M., (2002) J. Am. Chem. Soc, 124, p. 240
- Böhmer, M.R., Koopal, L.K., Janssen, R., Lee, E.M., Thomas, R.K., Rennie, A.R., (1992) Langmuir, 8, p. 2228
- Tiberg, F.J., (1996) J. Chem. Soc. Faraday Trans, 92, p. 531
- Forzani, E.S., Otero, M., Pérez, M.A., López Teijelo, M., Calvo, E.J., (2002) Langmuir, 18, p. 4020
- De Feijter, J.A., Benjamins, J., Veer, F.A., (1978) Biopolymers, 17, p. 1759
- Piehler, J., Brecht, A., Hehl, K., Gauglitz, G., (1999) Colloids Surf. B: Biointerf, 13, p. 325
- Cullen, D.C., Brown, R.G.W., Lowe, C.R., (1987) Biosensors, 3, p. 211
- Lu, H.C., Chen, H.M., Lin, Y.S., Lin, J.W., (2000) Biotechnol. Prog, 16, p. 116
- Ostuni, E., Chapman, R.G., Liang, M.N., Meluleni, G., Pier, G., Ingber, D.E., Whitesides, G.M., (2001) Langmuir, 17, p. 6336
- Bergstrom, J., Löfas, S., Johnsson, B., (1993), USP 5242828; Walczak, M.M., Popenoe, D.D., Deinhammer, R.S., Lamp, B.D., Chung, C., Porter, M.D., (1991) Langmuir, 7, p. 2687
- Walczac, M.M., Aves, C.A., Lamp, B.D., Porter, M.D., (1995) J. Electroanal. Chem, 396, p. 103
- El-Deab, M., Ohsaza, T., (2004) Electrochim. Acta, 49, p. 2189
- Raiber, K., Terfort, A., Benndorf, C., Krings, N., Strehblow, H.H., (2005) Surf. Sci, 595, p. 56
- Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., (2001) Langmuir, 17, p. 1225
- Osterberg, E., Bergstrom, K., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., Van Alstine, J.M., Harris, J.M., (1995) J. Biomed. Mater. Res, 29, p. 741. , and references therein
- Priano, G., Battaglini, F., (2005) Anal. Chem, 77, p. 4976
- Bartlett, P.N., Tebbutt, B.L.P., Tyrrell, C.H., (1992) Anal. Chem, 64, p. 138
Citas:
---------- APA ----------
Pallarola, D., Domenianni, L., Priano, G. & Battaglini, F. (2007) . A protein-resistant matrix for electrochemical based recognition assays. Electroanalysis, 19(6), 690-697.http://dx.doi.org/10.1002/elan.200603801
---------- CHICAGO ----------
Pallarola, D., Domenianni, L., Priano, G., Battaglini, F. "A protein-resistant matrix for electrochemical based recognition assays" . Electroanalysis 19, no. 6 (2007) : 690-697.http://dx.doi.org/10.1002/elan.200603801
---------- MLA ----------
Pallarola, D., Domenianni, L., Priano, G., Battaglini, F. "A protein-resistant matrix for electrochemical based recognition assays" . Electroanalysis, vol. 19, no. 6, 2007, pp. 690-697.http://dx.doi.org/10.1002/elan.200603801
---------- VANCOUVER ----------
Pallarola, D., Domenianni, L., Priano, G., Battaglini, F. A protein-resistant matrix for electrochemical based recognition assays. Electroanalysis. 2007;19(6):690-697.http://dx.doi.org/10.1002/elan.200603801
